Tissue Microarray Validation: A Methodologic Study with Special Reference to Lung Cancer

Karlsson, Christina; Bodin, Lennart; Piehl-Aulin, Karin; Karlsson, Mats G.

doi:10.1158/1055-9965.epi-08-0743

Cited by 27 publications

(21 citation statements)

References 32 publications

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“…Furthermore, TMA technology provides great economy of research resources, personnel, and tissue specimens. It is currently the technique of choice for high throughput research (15,(18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

Carbonic Anhydrase IX is Not a Predictor of Outcomes in Non-Metastatic Clear Cell Renal Cell Carcinoma - A Digital Analysis of Tissue Microarray

et al. 2013

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Introduction:The knowledge about the molecular biology of clear cell renal cell carcinoma (ccRCC) is evolving, and Carbonic Anhydrase type IX (CA-IX) has emerged as a potential prognostic marker in this challenging disease. However, most of the literature about CA-IX on ccRCC comes from series on metastatic cancer, with a lack of series on non-metastatic cancer. The objective is to evaluate the expression of CA-IX in a cohort of non-metastatic ccRCC, correlating with 1) overall survival, and 2) with established prognostic parameters (T stage, tumor size, Fuhrman nuclear grade, microvascular invasion and peri-renal fat invasion). Materials and Methods: This is a retrospective cohort study. We evaluated 95 patients with non-metastatic clear cell renal cell carcinoma, as to the expression of CA-IX. The analyzed parameters where: overall survival (OS), TNM stage, tumor size (TS), Fuhrman nuclear grade (FNG), microvascular invasion (MVI), peri-renal fat invasion (PFI). We utilized a custom built tissue microarray, and the immunoexpression was digitally quantified using the Photoshop® software. Results: The mean follow-up time was 7.9 years (range 1.9 to 19.5 years).The analysis of CA-IX expression against the selected prognostic parameters showed no correlation.

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Section: Discussionmentioning

confidence: 99%

Carbonic Anhydrase IX is Not a Predictor of Outcomes in Non-Metastatic Clear Cell Renal Cell Carcinoma - A Digital Analysis of Tissue Microarray

et al. 2013

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“…As the representativeness of the TMA plays a critical role particularly in focally expressed antigens, such as BCRP1, the TMA was prepared according to recent validation data in lung cancer and mesothelioma that demonstrated that three to four tissue cores per sample are sufficient to produce reliable results [35][36][37]. Moreover, in half of the cases, the results of the TMA were compared with corresponding whole tissue sections and showed full concordance.…”

Section: Discussionmentioning

confidence: 99%

Stage-specific embryonic antigen-4is expressed in basaloid lung cancer and associated with poor prognosis

et al. 2012

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Basaloid carcinoma represents a rare variant of nonsmall cell lung cancer (NSCLC), which has shown a poor prognosis in a number of studies. Although it is considered to derive from a pluri-or multipotent pulmonary stem cells, little is known about the expression and clinical significance of stem cell antigens in this variant.Stage-specific embryonic antigen-4 (SSEA-4) was analysed by immunohistochemistry in 38 patients with resected early-stage basaloid NSCLC who had a median follow-up of 72.9 months. The expression of SSEA-4 was related to clinico-pathological characteristics, to the expression of the adult stem cell antigens CD117, CD133 and breast cancer resistance protein 1 (BCRP1), and to prognosis.SSEA-4 was positive in 37% of the specimens and showed no association with clinicopathological characteristics or the expression of adult stem cell antigens. Cox proportional hazards regression analysis revealed a 6.0-fold increased risk of relapse (p50.001) and a 4.2-fold increased risk of disease-related mortality (p50.017) in SSEA-4-positive patients, while SSEA-4-negative patients showed a prognosis comparable with that of other early-stage NSCLC.SSEA-4 is expressed in a fraction of basaloid NSCLC and is associated with poor prognosis.

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“…We collected tissues by sampling one representative tissue core, 1 mm in diameter chosen from each formalin-fixed, paraffin-embedded tumor specimen (27)(28)(29)(30). The sections of tissue microarrays (TMAs) were dewaxed using xylene and rehydrated through graded alcohols.…”

Section: Methodsmentioning

confidence: 99%

Epithelial–Mesenchymal Transition in Non Small-cell Lung Cancer

2017

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Abstract. Background/Aim: Lung cancer is the first cause of cancer related deaths in both males and females. Epithelialmesenchymal transition (EMT) is a reversible process by which epithelial cells transform to mesenchymal stem cells by losing their cell polarity and cell-to-cell adhesionLung cancer is the first cause of cancer-related deaths in both males and females. It is a fatal disease and most patients with lung cancer will die of their disease (1). Two main types of lung cancer exist: non-small-cell lung cancer (NSCLC) accounting for 85-90% of lung cancer cases, and small-cell lung cancer (SCLC), whose incidence seems to have decreased over the past decades (2). The current therapeutic approach for lung cancer includes surgical tumor removal, platinum-based chemotherapy, targeted therapy, immunotherapy and radiation therapy. Improvements in clinical and molecular understanding of the disease have resulted in novel therapeutic approaches. Multiple genetic and epigenetic abnormalities results in both oncogene activation [epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK)] and inactivation of tumor-suppressor genes [transcription factor (p53) and retinoblastoma protein (RB)] (3). Moreover, angiogenesis seems to play an important role in the process of invasion and metastasis in lung cancer (4). Other molecular and cellular processes of lung cancer include genomic instability, escape from apoptosis, cell immortalization, abnormalities in immune response and epithelial-mesenchymal transition (EMT) (5-9).

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Tissue Microarray Validation: A Methodologic Study with Special Reference to Lung Cancer

Cited by 27 publications

References 32 publications

Carbonic Anhydrase IX is Not a Predictor of Outcomes in Non-Metastatic Clear Cell Renal Cell Carcinoma - A Digital Analysis of Tissue Microarray

Carbonic Anhydrase IX is Not a Predictor of Outcomes in Non-Metastatic Clear Cell Renal Cell Carcinoma - A Digital Analysis of Tissue Microarray

Stage-specific embryonic antigen-4is expressed in basaloid lung cancer and associated with poor prognosis

Epithelial–Mesenchymal Transition in Non Small-cell Lung Cancer

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