Tissue microarray: physical and chemical parameters involved in the construction of
            recipient blocks

Santos, Magda Guadalupe dos; Robeldo, Thaiane; Castañeda, Edwin David; Pagliarone, Ana Carolina; Pinto, Karina Nogueira Zambone; Borra, Ricardo Carneiro

doi:10.5935/1676-2444.20170041

Cited by 2 publications

(7 citation statements)

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“…Another study from 2017 had similar problems with the skin of swiss mice [10]. Insufficient quality of TMA slides of spleen parenchyma in about one third of all samples has been described in a previous study [10]. By contrast, in our analysis, only 0.9% (5/550) core loss was recorded for spleen parenchyma and no significant issues regarding the quality of punches were detected.…”

Section: Core Losssupporting

confidence: 71%

“…Three studies showed a lower amount of core loss with a diameter of 2.0 mm as compared to 1.0 [5,21], and 0.6 mm diameters [19]. The opposite was found in a study with better results for a 1.0 mm diameter when compared to 1.5 or 0.6 mm [10]. In the present study, good results with 2 and 1.5 mm diameters were obtained.…”

Section: Core Losscontrasting

confidence: 49%

“…The higher numbers in the case of skin tissue might be due to the fact that skin tissue in Wistar rats is rather rigid and quite tough. Another study from 2017 had similar problems with the skin of swiss mice [10]. Insufficient quality of TMA slides of spleen parenchyma in about one third of all samples has been described in a previous study [10].…”

Section: Core Lossmentioning

confidence: 83%

“…When using a distance of 0.7 mm between the punching cores, it is possible to insert up to 112 cores with a diameter of 1.5 mm and 66 cores with a diameter of 2 mm also according to the manufacturer's specifications. This grid design was chosen to achieve a high number of punches that can be inserted into a single recipient block without compromising the sectioning process by tight margins [10,18,[31][32][33].…”

Section: Determination Of Punching Diametermentioning

confidence: 99%

“…With the TMA method, it is possible to process several hundred tissue samples simultaneously and under identical conditions (incubation time, temperature, concentration of reagents) [3]. Further advantages include cost and time efficiency [2][3][4][5][6], evaluation under identical conditions [3,[5][6][7][8] and preservation of donor specimen [2,7,[9][10][11][12]. The combination of digital microscopy to select regions to punch using Digital Image Analysis (DIA) with fully automated tissue microarrays have led to the development of a new era in TMA technique and coined the term next-generation Tissue MicroArray (ngTMA ® ) [11,13].…”

Section: Introductionmentioning

confidence: 99%

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Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

et al. 2021

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This study aimed to investigate accuracy in different sectional planes of the TMA Grand Master (3DHISTECH) Workstation in various soft tissue samples collected from Wistar rats. A total of 108 animals were sacrificed and 963 tissue specimens collected from 12 soft-tissue types. A total of 3307 tissue cores were punched and transferred into 40 recipient TMA blocks. Digital image analysis was performed. Core loss showed a significant correlation with tissue type and was highest in skin tissue (p < 0.001), renal medulla and femoral artery, nerve, and vein bundle (p < 0.01). Overall, 231 of 3307 tissue cores (7.0%) were lost. Hit rate analysis was performed in 1852 punches. The target was hit completely, partially and missed totally by 89.4%, 7.2% and 2.2%. A total of 54.5% of punches had good accuracy with less than 200 µm deviation from the centre of the targeted region and 92.6% less than 500 µm. Accuracy decreases with greater sectional depth. In the deepest sectional plane of roughly 0.5 mm median depth, almost 90% of cores had a deviation below 500 µm. Recommendations for automated TMA creation are given in this article. The ngTMA®-method has proven accurate and reliable in different soft tissues, even in deeper sectional layers.

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Section: Core Losssupporting

confidence: 71%

Section: Core Losscontrasting

confidence: 49%

Section: Core Lossmentioning

confidence: 83%

Section: Determination Of Punching Diametermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

et al. 2021

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A Simplified Method of Manually Constructing Small Format Tissue Microarray for Use in Resource-Constrained Settings

Palo

2023

Iran J Pathol

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Background & Objective: Tissue microarray (TMA) is a method of harvesting small tissue cores from a number of donor paraffin tissue blocks and arraying them in a recipient paraffin block. It has numerous advantages and applications but is expensive. This study aimed to develop a simple yet efficient method of manual, small-format TMA block construction. Methods: Disposable skin punch biopsy needles were used to manually core out 4-mm cylinders from the archival donor blocks comprising tissue from 60 thyroidectomy specimens. These cores were oriented in the embedding cassette in accordance with the grid design. The molten wax was slowly dispensed and allowed to be set. Sectioning, mounting, and hematoxylin and eosin (H&E) staining were performed by a conventional method. Immunohistochemical studies, using HBME-1, CK19, and S100 antibodies, were also performed on these tissue array sections. Results: There was no core loss during processing. Technical issues like core tilt and floatation were easily tackled. Morphological identification, histological typing, and immunohistochemical analysis could be satisfactorily performed in these TMA sections. Donor blocks did not break after punching. Conclusion: This TMA construction method is simple, feasible, easily reproducible, and time-saving. It can serve as an excellent cost-effective alternative for resource-poor laboratories for carrying out immunohistochemical studies.

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Tissue microarray: physical and chemical parameters involved in the construction of recipient blocks

Cited by 2 publications

References 0 publications

Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

A Simplified Method of Manually Constructing Small Format Tissue Microarray for Use in Resource-Constrained Settings

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