Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma

Fonseca, Felipe Paiva; Andrade, Bruno Augusto Benevenuto de; Rangel, Ana Lúcia Carrinho Ayroza; Coletta, Ricardo D.; Lopes, Márcio Ajudarte; Almeida, Oslei Paes de; Vargas, Pablo Agustı́n

doi:10.1016/j.oooo.2013.08.029

Cited by 34 publications

(33 citation statements)

References 25 publications

(25 reference statements)

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“…Moreover, analysis of the cytokeratin focused on the biphasic cell differentiation of myoepithelial cell in pleomorphic adenoma has been conducted hence CK7 and CK13 were selected this time 31, 32. In the present study, the small cuboidal cells in solid tumor cell nests showed strong positive reaction to Wnt as well as to β-catenin with nuclear reaction recognized in the latter protein.…”

Section: Discussionmentioning

confidence: 63%

Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas

et al. 2014

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There are well known that Wnt signaling was some roles of cell differentiation at the development tissues, especially the oral and maxillofacial regions of some developmental stages. Therefore, to determine Wnt signaling in the pleomorphic adenoma tissues, we examined. The expression of Wnt1 and β-catenin as well as the distribution of various cytoskeletal proteins CK7 and CK13 was examined in 30 cases of pleomorphic adenoma by immunohistochemistry. Wnt1 was detected in almost all tumor cells. The peripheral columnar cells in squamous metaplasia and small cuboidal cells in duct-like structures were strongly positive to Wnt1. Although β-catenin was clearly localized on the cell membrane of tumor cells, nuclear translocation was observed in small cuboidal cells and in some basaloid cells. The immunofluorescent staining pattern of Wnt1 and CK7 as well as Wnt1 and CK13 was consistent with IHC results. Thus, in pleomorphic adenoma, Wnt is involved in tumor cell differentiation of peripheral columnar cells forming solid nests and small peripheral columnar cells forming duct-like structures. Moreover, among the three currently known Wnt pathways, β-catenin is the suggested pathway working during cell differentiation. Furthermore, peripheral columnar cells in solid tumor nests and in squamous metaplasia are governed by another Wnt pathway other than β-catenin. Therefore, Wnt signaling through β-catenin pathway may be involved in the 'mixed' differentiation characteristic of pleomorphic adenoma although another pathway may also be possibly working in other parts of the tumor tissue.

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Section: Discussionmentioning

confidence: 63%

Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas

et al. 2014

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“…Tissue microarray (TMA) construction was done as previously described (Fonseca et al, 2014). Briefly, tumor areas were selected and marked on hematoxylineosin-stained sections using an objective marker (Nikon Corp, Tokyo, Japan).…”

Section: Tma Constructionmentioning

confidence: 99%

Vascular endothelial growth factor immunoexpression is increased in malignant salivary gland tumors

Fonseca

Basso

Mariano

et al. 2015

Annals of Diagnostic Pathology

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“…Ki-67 is thus the proliferation marker used to assess various types of tumors [16,17]. Expression of Ki-67 may also be used as a proliferation marker and prognostic tool in epithelial salivary gland tumor assessment in adults [18,19]. Faur suggests that Ki-67 expression may be especially useful in assessment of extremely proliferative forms of salivary gland tumors, both benign and malignant [5].…”

Section: Discussionmentioning

confidence: 99%

Comparison between immunohistochemical expression of Ki-67 and MCM-3 in major salivary gland epithelial tumors in children and adolescents. Preliminary study

Zieliński¹,

Kobos²,

Zakrzewska³

2016

pjp

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While Ki-67 expression is frequently used as an indicator of tumor cell proliferation, alternative markers have also been proposed. Possible alternative indicators of proliferation are the minichromosome maintenance (MCM) proteins, whose levels are inversely associated with tumor cell differentiation. The aim of this preliminary study was to compare the levels of Ki-67 and MCM-3 expression in major salivary gland epithelial tumors in all children and adolescents who underwent surgery in our department in the years 2009-2014. The histopathological diagnosis of the subjects was reviewed, as well as the expression of Ki-67 and MCM-3 in post-op specimens of the tumors. The normality of data was checked with the Shapiro-Wilk test. The t test for independent variables or the U test was used as appropriate to determine statistically significant differences in the expression of Ki-67 and MCM-3. Five cases of pleomorphic adenoma, one of myoepithelioma, one of basal cell adenoma and one of mucoepidermoid carcinoma were identified. Significantly greater MCM-3 than Ki-67 expression was observed in every case. The results of our preliminary study emphasize the need for future research on MCM-3 as a sensitive proliferation marker, providing an alternative to Ki-67, in cases of various major salivary gland epithelial tumors in children and adolescents.

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Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma

Abstract: Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study.

Cited by 34 publications

References 25 publications

Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas

Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas

Vascular endothelial growth factor immunoexpression is increased in malignant salivary gland tumors

Comparison between immunohistochemical expression of Ki-67 and MCM-3 in major salivary gland epithelial tumors in children and adolescents. Preliminary study

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