Tissue mast cells. Studies with a histamine‐liberator of low toxicity (compound 48/80)

Riley, James F.; West, G. B.

doi:10.1002/path.1700690135

Cited by 163 publications

(51 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hematoxylin and eosin staining was performed with Harris' hematoxylin (Surgipath, Richmond, IL) for 30 s and eosin (Surgipath) for 2 min. Toluidine blue staining was carried out by briefl y submerging tissue sections in 0.1% aqueous toluidine blue (EMS, Hatfi eld, PA) ( 27 ). Mast cells, solitary ATM, and CLS were counted in 20 high-power fi elds (400×), and their density was expressed as cells or CLS per mm 2 of tissue section.…”

Section: Histologymentioning

confidence: 99%

See 1 more Smart Citation

Mast cells, macrophages, and crown-like structures distinguish subcutaneous from visceral fat in mice

Altintas

Azad

Nayer

et al. 2011

Journal of Lipid Research

160

View full text Add to dashboard Cite

This article is available online at http://www.jlr.org is of grave consequence to human health. In fact, cardiovascular diseases, mostly as a result of long-standing metabolic dysregulations, are the leading cause of morbidity and mortality in many parts of the world.The pathogenesis of obesity-linked insulin resistance is only partially understood. Accumulating evidence has revealed a strong association between obesity-linked insulin resistance and infl ammation ( 2 ). Tumor necrosis factor-a (TNF-a ), a prototypical pro-infl ammatory cytokine, was upregulated in the epididymal fat of obese rodents, and neutralization of TNF-a ameliorated insulin resistance ( 3 ). Moreover, obese mice lacking TNF-a demonstrated improved insulin sensitivity ( 4 ). In addition, increased numbers of macrophages and their transcripts were reported in the epididymal fat of genetically and dietinduced obese (DIO) mice ( 5, 6 ). These adipose tissue macrophages (ATM) were found scattered between adipocytes (herein referred to as solitary ATM ) or forming clusters around adipocytes. Based on ultrastructural and immunohistochemical alterations, it was argued that most ATM in obese mice and humans surround dead adipocytes, forming so-called crown-like structures (CLS) ( 7 ). Subsequently, we reported that CLS were distributed differentially in abdominal fat depots of DIO mice ( 8 ). Consistent with our fi ndings, it was recently reported that CLS were also more prevalent in visceral than subcutaneous fat of leptin-defi cient ob/ob and leptin receptor-defi cient db/db mice ( 9 ).Although macrophages are critical in innate and adaptive immunity, most immune responses are the result of interplay between multiple cell types and mediators of the immune system ( 10 ). Therefore, it comes as no surprise to learn that regulatory T cells ( 11 ), CD8+ effector T cells Over the past several decades a steady increase in the prevalence of obesity across the continents, especially in the US, has been observed ( 1 ). Increased caloric intake, mostly due to consumption of a high-fat diet, and decreased physical activity seem to be major contributors. Insulin resistance, hypertension, and dyslipidemia often accompany obesity. The constellation, referred to as metabolic syndrome, This work was supported by generous funds from the Katz Family Foundation.

show abstract

Section: Histologymentioning

confidence: 99%

“…2A -C ). When stained with toluidine blue, a basic aniline dye, mast cells were readily identifi ed as cells with ample cytoplasm packed with large purple granules ( 27 ) ( Fig. 2D-F ).…”

Section: Mast Cells Solitary Atm and Cls Were Demonstrated In Abdommentioning

confidence: 99%

Mast cells, macrophages, and crown-like structures distinguish subcutaneous from visceral fat in mice

Altintas

Azad

Nayer

et al. 2011

Journal of Lipid Research

160

View full text Add to dashboard Cite

show abstract

“…The second distal colonic segment was used for histamine release measurement. Briefly, the colonic segment (0·5 cm) was incubated for 1 h in a Ringer solution containing compound 48Ï80 (1·5 mg ml¢), a potent rat mast cell secretagogue (Riley & West, 1955). The histamine concentration in the supernatant and in the blood sample were measured with a radioimmunoassay (RIA) kit using polyclonal histamine antibodies (Immunotech, Marseille, France).…”

Section: Identification Of Mast Cells and Measurement Of Histamine Rementioning

confidence: 99%

Involvement of interleukin‐1, prostaglandins and mast cells in rectal distension‐induced colonic water secretion in rats

Eutamène¹,

Théodorou²,

Vergnolle³

et al. 1998

The Journal of Physiology

View full text Add to dashboard Cite

In vivo rectal distension (RD) induces a neurally mediated colonic net water hypersecretion in rats. Interleukin‐1β (IL‐1β) also induces neural colonic water hypersecretion involving the release of prostaglandins (PGs) and a mast cell degranulation in rats. This study investigated in vivo the role of IL‐1, PGs and mast cells in RD‐induced colonic hypersecretion. Proximal colonic net water flux was determined using [14C]polyethylene glycol (PEG) 4000 (mol. wt, 4000) in anaesthetized rats. On strips taken from the distal colon: (i) a histological analysis was performed to determine the number of mucosal mast cells (MMC); and (ii) histamine levels were measured by radioimmunoassay after stimulation with compound 48/80. RD induced a net colonic water secretion that was blocked by i.c.v. administration of IL‐1ra (an IL‐1 receptor antagonist) and indomethacin, and by systemic treatment with doxantrazole and indomethacin. RD decreased the number of resident mast cells and the release of histamine from the distal colonic strips. Moreover, using SDS‐PAGE immunoblotting the expression of IL‐1β was detected in the brain. These results suggest that, in rats, RD induces colonic net water hypersecretion by the activation of a neuro‐immunological reflex pathway, involving brain IL‐1β, PG release and peripheral mast cell degranulation.

show abstract

“…It is well known that compound 48/80 is a highly efficient histamine liberator in the rat, acting mainly on the histamine in the mast cells of the skin (Riley & West, 1955a, b). Reserpine is likewise capable of removing 5-HT from the blood platelets (Shore, Pletscher, Tomich, Kuntzman & Brodie, 1956).…”

Section: -Ht Histamine and Mast Cells 513mentioning

confidence: 99%

“…This is known to remove almost completely the histamine from the skin, and especially the ears, which can be used as an index of the effect (Riley & West, 1955a).…”

Section: -Ht Histamine and Mast Cells 513mentioning

confidence: 99%

5‐Hydroxytryptamine and histamine in mast cells of the mouse and rat

Cass¹,

Marshall²,

Riley³

1958

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

Tissue mast cells. Studies with a histamine‐liberator of low toxicity (compound 48/80)

Cited by 163 publications

References 7 publications

Mast cells, macrophages, and crown-like structures distinguish subcutaneous from visceral fat in mice

Mast cells, macrophages, and crown-like structures distinguish subcutaneous from visceral fat in mice

Involvement of interleukin‐1, prostaglandins and mast cells in rectal distension‐induced colonic water secretion in rats

5‐Hydroxytryptamine and histamine in mast cells of the mouse and rat

Contact Info

Product

Resources

About