Tissue levels and biomarkers of organic contaminants in feral and caged chub (Leuciscus cephalus) from rivers in the West Midlands, UK

Winter, Matthew J.; Verweij, F.; Garofalo, Elisabetta; Ceradini, S.; McKenzie, David J.; Williams, Mark A.; Taylor, E. W.; Butler, Patrick J.; Oost, Ron van der; Chipman, J.K.

doi:10.1016/j.aquatox.2005.05.001

Cited by 39 publications

(31 citation statements)

References 33 publications

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“…Also the biomarker measurements provide no evidence for strong toxic pressure. While the VTG biomarker revealed no estrogenic exposure at all, the levels of the CYP1A/ EROD biomarker corresponded to levels reported for chub sampled at chronically polluted field sites in the United Kingdom or Belgium [8,9]. The presence of PAH metabolites in the bile suggests that at least part of the AhR ligand exposure is caused by PAHs.…”

Section: Discussionsupporting

confidence: 64%

Assessing relationships between chemical exposure, parasite infection, fish health, and fish ecological status: A case study using chub (Leuciscus cephalus) in the Bílina River, Czech Republic

Wenger

Ondračková

Machala

et al. 2009

Enviro Toxic and Chemistry

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Multiple stressor scenarios, as they are relevant in many watersheds, call for approaches extending beyond conventional chemical-focused approaches. The present study, investigated the fish population, represented by chub (Leuciscus cephalus), in the Bílina River (Czech Republic), which is impacted by various pollution sources and might pose a risk on the fish population. To confirm or reject this hypothesis it was examined whether there exists an association between abundance of chub and exposure to toxic chemicals as well as natural stressors, represented by parasites, and whether health-related suborganismal traits, namely, organ indices, tissue histopathology, and immune parameters, would help in revealing relationships between stressor impact and population status. Toxic pressure was assessed by the toxic unit approach, which gives an integrative estimate of toxic effect concentrations and by measuring the biomarkers cytochrome P4501A and vitellogenin, which indicate exposure to bioavailable arylhydrocarbon- or estrogen receptor ligands. Parasite pressure was estimated by determining abundance and species composition of ecto- and endoparasites of chub. Chub abundance was high upstream in the Bílina, low to zero in the middle stretches, and increased again downstream. Toxic pressure increased in the downstream direction, while parasite intensity decreased in this direction. Health status of chub did not differ clearly between up-, middle-, and downstream sites. Thus, it appears that neither toxic pressure nor parasite pressure nor their combination translates into a change of chub health status. By using varied assessment tools, this study provides evidence against a presumed causative role of toxicants impairing the fish ecological status of the Bílina River.

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Section: Discussionsupporting

confidence: 64%

Assessing relationships between chemical exposure, parasite infection, fish health, and fish ecological status: A case study using chub (Leuciscus cephalus) in the Bílina River, Czech Republic

Wenger

Ondračková

Machala

et al. 2009

Enviro Toxic and Chemistry

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“…These findings are in positive correlation with DNA damage levels observed by the comet assay in our study. Winter et al (2005) found a positive correlation of percentage tail DNA with EROD activity, as well as with total muscle organochlorine pesticides and PCBs in the feral chub. In another study, DNA damage detected by the comet assay in feral chub was positively correlated with EROD, total muscle OCPs, and with specific PCB congeners, while in caged fish DNA damage was correlated only with EROD activity (Winter et al 2004).…”

Section: Comet Assaymentioning

confidence: 92%

“…Due to its availability and sensitivity, it has been used in biomonitoring of pollution-related toxicity in some European rivers (Devaux et al 1998;Larno et al 2001;Winter et al 2004Winter et al , 2005Dragun et al 2007;Krča et al 2007) using different cellular and molecular biomarkers.…”

Section: Introductionmentioning

confidence: 99%

DNA integrity of chub erythrocytes (Squalius cephalus L.) as an indicator of pollution-related genotoxicity in the River Sava

Pavlica

Štambuk

Malović

et al. 2010

Environ Monit Assess

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An alkaline comet assay and a micronucleus test were carried out on erythrocytes of the European chub, Squalius cephalus L., collected in spring and autumn in 2005 and 2006 at three sampling sites in River Sava, near Zagreb, Croatia. The results of comet assay showed the lowest genotoxic influence at the least polluted site, while higher DNA damage was observed at the polluted sites. Although the basal levels of DNA damage were elevated, a clear gradation of DNA damage was found due to pollution intensity in all sampling periods. The lowest cytogenetic damage as revealed by the micronucleus test (MNT) was observed as well at the least polluted site. High variations in MN frequency were observed between sampling periods, although the number of micronucleated erythrocytes was consistently the highest one at the polluted site. The comet assay as a biomarker of genotoxic effect exhibited higher sensitivity in discriminating the genotoxic capacity of studied polluted sites while the MNT was less sensitive. However, both tests should be used together in biomonitoring studies because they can reveal different aspects of DNA damage; comet assay, the early event of genotoxic exposure, and MNT, its final result as a mutagenic potential.

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“…Concentrations of PCBs were found to be in the range of 0.20–2.5 ng g −1 wet weight in the seafoods around Shanghai, China (Nakata et al, ). Total PCBs measured in winter 2002 feral and caged fish collected from the River Tame around Birmingham were as high as 18,000 ng g −1 lipid (Winter et al, ). The estimated total daily dietary intakes of PCBs (12,372.9 ng day −1 ) in the large e‐waste disassembly sites were approximately two to three times higher than those in the control site in the Zhejiang Province of China (Zhao et al, ).…”

Section: Introductionmentioning

confidence: 99%

Aroclor 1254 causes atrophy of exocrine pancreas in mice and the mechanism involved

Lin

Sun

et al. 2014

Environ. Toxicol.

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Polychlorinated biphenyls (PCBs) are a class of organic pollutants that have been linked to pancreatic disease. However, their role in affecting the exocrine function of pancreas and the underlying mechanism remains elusive. In the present study, male C57 mice were treated with Aroclor 1254, a commercially available PCBs mixture, at a dosage of 0.5, 5, 50, or 500 μg kg(-1) every 3 days by oral gavage. Decrease in pancreas/soma index and acinar atrophy were observed in the mice after exposure for 50 days. Aroclor 1254 exposure significantly decreased the PCNA-positive cells in the pancreatic acini in a dose-dependent manner. In addition, western blot analysis showed that PCNA expression was decreased in pancreas in the presence of Aroclor 1254, which suggests that Aroclor 1254 suppresses cell proliferation. TUNEL-positive apoptotic cells as well as the expression of Bcl2, BclXL, BAX, and Bad of exocrine pancreas did not show significant changes in the treated mice, indicating that Aroclor 1254 has no effect on apoptosis. We also found that phosphorylation of ERK1/2, P90RSK1 and Bad was increased in the treated groups; this compensatory activation of phosphorylation in ERK1/2-P90RSK1-Bad signaling cascade could protect cell from apoptosis to maintain the cell numbers and function of exocrine pancreas. Moreover, we found that the expression of Kras and TNFα was increased in the pancreas, indicating that Aroclor 1254 exposure could result in increased risk of inflammation and carcinoma. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 671-678, 2016.

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Tissue levels and biomarkers of organic contaminants in feral and caged chub (Leuciscus cephalus) from rivers in the West Midlands, UK

Cited by 39 publications

References 33 publications

Assessing relationships between chemical exposure, parasite infection, fish health, and fish ecological status: A case study using chub (Leuciscus cephalus) in the Bílina River, Czech Republic

Assessing relationships between chemical exposure, parasite infection, fish health, and fish ecological status: A case study using chub (Leuciscus cephalus) in the Bílina River, Czech Republic

DNA integrity of chub erythrocytes (Squalius cephalus L.) as an indicator of pollution-related genotoxicity in the River Sava

Aroclor 1254 causes atrophy of exocrine pancreas in mice and the mechanism involved

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