Tissue ingrowth and degradation of two biodegradable porous polymers with different porosities and pore sizes

Tienen, T.G. van; Heijkants, R. G. J. C.; Buma, P.; Groot, J.H. de; Pennings, A. J.; Veth, R.P.H.

doi:10.1016/s0142-9612(01)00280-0

Cited by 239 publications

(169 citation statements)

References 19 publications

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“…These results confirm that a porous scaffold is superior to a non-porous scaffold for promoting angiogenesis and that increasing scaffold porosity increases vascularisation. Furthermore, these reported results agree with van Tienen and colleagues who compared the tissue ingrowth of two 26 polyesterurethane scaffolds with varying porosity 45 . The authors attributed the enhanced fibrovascular ingrowth with increasing scaffold porosity.…”

Section: Discussionsupporting

confidence: 88%

Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture

et al. 2009

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The aim of this study was to investigate an in-vivo tissue response to a biodegradable polyesterurethane, specifically the cellular and angiogenic response evoked by varying implant architectures in a subcutaneous rabbit implant model. A synthetic biodegradable polyesterurethane was synthesised and processed into three different configurations; a non-porous film, a porous mesh and a porous membrane. Glutaraldehyde cross-linked bovine pericardium was used as a control. Sterile polyesterurethane and control samples were implanted subcutaneously in six rabbits, (n=12). The rabbits were sacrificed at 21 and 63 days and the implant sites were sectioned and histologically stained using haemotoxylin and eosin (H&E), Masson's trichrome, picosirius red and immunostain CD31. The tissue-implant interface thickness was measured from the H&E slides.Stereological techniques were used to quantify the tissue reaction at each time point that included volume fraction of inflammatory cells, fibroblasts, fibrocytes, collagen and the degree of vascularisation. Stereological analysis inferred that porous scaffolds with regular topography are better tolerated in-vivo compared to non-porous scaffolds, while increasing scaffold porosity promotes angiogenesis and cellular infiltration. The results suggest that this biodegradable polyesterurethane is better tolerated in-vivo than the control and that structural variants of biodegradable polyesterurethane in-vivo evoke a cellular and angiogenic response that is dictated by architecture.

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Section: Discussionsupporting

confidence: 88%

Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture

et al. 2009

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“…In sheep models, the meniscus transplant horns are generally fixed with transosseous sutures. 9,15,20,21,23 Considering that most of the animal studies reports high incidence of implant lesions at longer follow-up, 9,15,23 we have decided to compare two different implant fixation techniques to determine a most suitable animal model for total meniscal substitution. The degree of damage seen in the meniscal implants at the completion of our study appears related to the implant fixation technique used.…”

Section: Discussionmentioning

confidence: 99%

“…Other porous polymer implants did not prevent cartilage degeneration or were not suitable as meniscal substitutes because of poor tissue ingrowth related to polymer degradation rate, and poor mechanical properties. [21][22][23] Tissue engineering has recently been proposed as a possible solution for meniscal regeneration. 15,[24][25][26][27][28] There have, however, been very few animal studies reported in the literature that have investigated the possibility of using cells in a partial or total meniscal substitute.…”

Section: Introduction Lmentioning

confidence: 99%

Tissue Engineering for Total Meniscal Substitution: Animal Study in Sheep Model

Kon

Chiari

Marcacci

et al. 2008

Tissue Engineering Part A

113

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Objective: The aim of the study was to investigate the use of a novel hyaluronic acid/polycaprolactone material for meniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of the implant with expanded autologous chondrocytes. Two different surgical implantation techniques in a sheep model were evaluated. Methods: Twenty-four skeletally mature sheep were treated with total medial meniscus replacements, while two meniscectomies served as empty controls. The animals were divided into two groups: cell-free scaffold and scaffold seeded with autologous chondrocytes. Two different surgical techniques were compared: in 12 animals, the implant was sutured to the capsule and to the meniscal ligament; in the other 12 animals, also a transtibial fixation of the horns was used. The animals were euthanized after 4 months. The specimens were assessed by gross inspection and histology.

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“…The scaffold pore structure has been observed to significantly affect cell binding and migration in vitro and influence the rate and depth of cellular ingrowth into the scaffold in vitro and in vivo [7,8]. Additionally, cell adhesion and activity has been observed to vary considerably depending on the cell type, as well as the scaffolds composition and pore size [9][10][11].…”

Section: Introductionmentioning

confidence: 99%