Tissue “Hypoxia” and the Maintenance of Leukemia Stem Cells

Sbarba, Persio Dello; Cheloni, Giulia

doi:10.1007/978-981-13-7342-8_6

Cited by 4 publications

(3 citation statements)

References 80 publications

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“…Pretreated hypoxic stem cells have altered gene expression, exhibit immature traits, and grow faster in vivo [14,15]. Increasing evidence has indicated that stem cells grow in hypoxic microenvironment and that they are dependent on the activity of hypoxia-inducible factor (HIF) to maintain low differentiation [14][15][16]. The mechanism of renal stem cells to alleviate renal interstitial fibrosis has been limited to experimental prediction due to a lack of recognized theories.…”

Section: Wwwbjbmsorgmentioning

confidence: 99%

“…The differentiation of both embryonic stem cells and progenitor cells can be inhibited in hypoxia. Pretreated hypoxic stem cells have altered gene expression, exhibit immature traits, and grow faster in vivo [ 14 , 15 ]. Increasing evidence has indicated that stem cells grow in hypoxic microenvironment and that they are dependent on the activity of hypoxia-inducible factor (HIF) to maintain low differentiation [ 14 - 16 ].…”

Section: Introductionmentioning

confidence: 99%

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The effect and molecular mechanism of hypoxia on proliferation and apoptosis of CD133+ renal stem cells

Liu

Qü

2020

Bosn J of Basic Med Sci

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Congenital hydronephrosis caused by ureteropelvic junction obstruction (UPJO) eventually leads to renal interstitial fibrosis and atrophy, after a series of pathophysiological problems. Renal repair after injury depends on renal stem cells. This study aimed to determine the expression of renal stem cell marker CD133 in children of different ages and the regulatory effect of stem cell microenvironment. Renal stem cells from children of different ages were identified and screened out by flow cytometry in the study. Children with hydronephrosis were divided into neonates, infants, preschool age, school age, and adolescents groups. A hypoxic cell model prepared with CoCl2 was developed to detect the effect of hypoxia on the proliferation and apoptosis of renal stem cells. The effect and molecular mechanism of hypoxia-inducible factor 1-alpha (HIF-1α) on the proliferation and apoptosis of renal stem cells were also explored. Both hypoxia and HIF-1α significantly promoted the proliferation of renal stem cells and inhibited cell apoptosis. HIF-1α could bind to the promoter region of proliferating cell nuclear antigen (PCNA) and PROM1 (CD133) to mediate their transcription and expression. The content of CD133+ renal stem cells was the highest in the neonatal group and it decreased with the increase of age. Taken together, this study clarified the effect of age on the content of human renal stem cells and determined the regulatory mechanism of hypoxia on renal stem cells. We expect our results to provide a research basis for the treatment and clinical application of renal stem cells.

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Section: Wwwbjbmsorgmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect and molecular mechanism of hypoxia on proliferation and apoptosis of CD133+ renal stem cells

Liu

Qü

2020

Bosn J of Basic Med Sci

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“…NSC and NPC self-renewal and differentiation are tightly controlled by the cellular microenvironment or "niche". For instance, high glucose concentrations inhibit NSC differentiation through oxidative endoplasmic reticulum stress [2], while physiological oxygen maintains stemness [3]. Oxygen levels not only maintain the undifferentiated state, but also modulate the proliferation, quiescence and fate commitment of NSCs [1].…”

Section: Introductionmentioning

confidence: 99%

Dimethyloxalylglycine (DMOG), a Hypoxia Mimetic Agent, Does Not Replicate a Rat Pheochromocytoma (PC12) Cell Biological Response to Reduced Oxygen Culture

2022

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Cells respond to reduced oxygen availability predominately by activation of the hypoxia-inducible factor (HIF) pathway. HIF activation upregulates hundreds of genes that help cells survive in the reduced oxygen environment. The aim of this study is to determine whether chemical-induced HIF accumulation mimics all aspects of the hypoxic response of cells. We compared the effects of dimethyloxalylglycine (DMOG) (a HIF stabiliser) on PC12 cells cultured in air oxygen (20.9% O2, AO) with those cultured in either intermittent 20.9% O2 to 2% O2 (IH) or constant 2% O2 (CN). Cell viability, cell cycle, HIF accumulation, reactive oxygen species (ROS) formation, mitochondrial function and differentiation were used to characterise the PC12 cells and evaluate the impact of DMOG. IH and CN culture reduced the increase in cell numbers after 72 and 96 h and MTT activity after 48 h compared to AO culture. Further, DMOG supplementation in AO induced a dose-dependent reduction in the increase in PC12 cell numbers and MTT activity. IH-cultured PC12 cells displayed increased and sustained HIF-1 expression over 96 h. This was accompanied by increased ROS and mitochondrial burden. PC12 cells in CN displayed little changes in HIF-1 expression or ROS levels. DMOG (0.1 mM) supplementation resulted in an IH-like HIF-1 profile. The mitochondrial burden and action potential of DMOG-supplemented PC12 cells did not mirror those seen in other conditions. DMOG significantly increased S phase cell populations after 72 and 96 h. No significant effect on PC12 cell differentiation was noted with IH and CN culture without induction by nerve growth factor (NGF), while DMOG significantly increased PC12 cell differentiation with and without NGF. In conclusion, DMOG and reduced oxygen levels stabilise HIF and affect mitochondrial activity and cell behaviour. However, DMOG does not provide an accurate replication of the reduced oxygen environments.

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Clinical strategies to inhibit tumor vascularization

Harris

2020

Tumor Vascularization

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Tissue “Hypoxia” and the Maintenance of Leukemia Stem Cells

Cited by 4 publications

References 80 publications

The effect and molecular mechanism of hypoxia on proliferation and apoptosis of CD133+ renal stem cells

The effect and molecular mechanism of hypoxia on proliferation and apoptosis of CD133+ renal stem cells

Dimethyloxalylglycine (DMOG), a Hypoxia Mimetic Agent, Does Not Replicate a Rat Pheochromocytoma (PC12) Cell Biological Response to Reduced Oxygen Culture

Clinical strategies to inhibit tumor vascularization

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