Tissue guidance without filopodia

Wacker, Andrin; Gerhardt, Holger; Phng, Li Kun

doi:10.4161/cib.28820

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…4b,d), well aligned with the secondary nanofibrous structures of the PLLA patterns, which have widths ranging between 100 and 400 nm (Figure 4c,d), well matching those of filopodia [45]. It is known that filopodia have an important role in sensing the external environment and it is hypothesized that they actively contribute to the contact guidance behavior of cells, although this point is under debate and the basic mechanisms involved are not yet clear [44,[46][47][48][49][50]. Standard investigations over contact guidance phenomena require large area nanostructures with appropriate sizes and topological features [51], and the currently available patterning techniques are not optimized for delivering at the same time aligned nanostructures on large substrate areas at low costs; in other words, these studies require to sustain non-negligible costs associated to the production of large area aligned nanostructured scaffolds.…”

Section: Discussionmentioning

confidence: 81%

Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies

Mohanraj

Puzzi

Capria

et al. 2016

Materials Science and Engineering: C

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Section: Discussionmentioning

confidence: 81%

Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies

Mohanraj

Puzzi

Capria

et al. 2016

Materials Science and Engineering: C

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“…In these experiments, the growth and patterning of intersegmental vessels were not disturbed by abolishing filopodia (using a low dose of Latrunculin B, which prevents F-actin polymerisation). Nevertheless, tip cells were still able to create lamellipodia, which, under these circumstances, might act as mediators of guided angiogenesis (Phng et al, 2013;Wacker et al, 2014).…”

Section: The Endothelial Tip-stalk Machinerymentioning

confidence: 99%

Retinal vasculature development in health and disease

Selvam

Kumar

Fruttiger

2018

Progress in Retinal and Eye Research

255

252

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Development of the retinal vasculature is based on highly coordinated signalling between different cell types of the retina, integrating internal metabolic requirements with external influences such as the supply of oxygen and nutrients. The developing mouse retinal vasculature is a useful model system to study these interactions because it is experimentally accessible for intra ocular injections and genetic manipulations, can be easily imaged and develops in a similar fashion to that of humans. Research using this model has provided insights about general principles of angiogenesis as well as pathologies that affect the developing retinal vasculature. In this review, we discuss recent advances in our understanding of the molecular and cellular mechanisms that govern the interactions between neurons, glial and vascular cells in the developing retina. This includes a review of mechanisms that shape the retinal vasculature, such as sprouting angiogenesis, vascular network remodelling and vessel maturation. We also explore how the disruption of these processes in mice can lead to pathology - such as oxygen induced retinopathy - and how this translates to human retinopathy of prematurity.

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“…However, in vivo evidence for the importance of VEGF gradients for the directed growth of vascular networks is sparse (Ruhrberg et al, 2002 ), and also in vitro , convincing evidence is largely absent (Bautch, 2012 ). Likewise, filopodia seems to be dispensable for vascular patterning (Wacker et al, 2014 ). In the expansion of lymphatic networks, similar directed sprouting can be observed, but e.g.…”

Section: Directing Regenerative Lymphatic Growth In Vivomentioning

confidence: 99%

Biology of Vascular Endothelial Growth Factor C in the Morphogenesis of Lymphatic Vessels

Rauniyar

Jha

Jeltsch

2018

Front. Bioeng. Biotechnol.

103

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Because virtually all tissues contain blood vessels, the importance of hemevascularization has been long recognized in regenerative medicine and tissue engineering. However, the lymphatic vasculature has only recently become a subject of interest. Central to the task of growing a lymphatic network are lymphatic endothelial cells (LECs), which constitute the innermost layer of all lymphatic vessels. The central molecule that directs proliferation and migration of LECs during embryogenesis is vascular endothelial growth factor C (VEGF-C). VEGF-C is therefore an important ingredient for LEC culture and attempts to (re)generate lymphatic vessels and networks. During its biosynthesis VEGF-C undergoes a stepwise proteolytic processing, during which its properties and affinities for its interaction partners change. Many of these fundamental aspects of VEGF-C biosynthesis have only recently been uncovered. So far, most—if not all—applications of VEGF-C do not discriminate between different forms of VEGF-C. However, for lymphatic regeneration and engineering purposes, it appears mandatory to understand these differences, since they relate, e.g., to important aspects such as biodistribution and receptor activation potential. In this review, we discuss the molecular biology of VEGF-C as it relates to the growth of LECs and lymphatic vessels. However, the properties of VEGF-C are similarly relevant for the cardiovascular system, since both old and recent data show that VEGF-C can have a profound effect on the blood vasculature.

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Tissue guidance without filopodia

Cited by 9 publications

References 37 publications

Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies

Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies

Retinal vasculature development in health and disease

Biology of Vascular Endothelial Growth Factor C in the Morphogenesis of Lymphatic Vessels

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