Tissue glutathione and cysteine levels in methionine-restricted rats

Richie, John P.; Komninou, Despina; Leutzinger, Yvonne; Kleinman, Wayne; Orentreich, Norman; Malloy, Virginia; Zimmerman, Jay A.

doi:10.1016/j.nut.2004.05.009

Cited by 79 publications

(70 citation statements)

References 34 publications

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“…Decreased mitochondrial ROs, serum glucose, insulin, and insulin-like growth factor-1 levels and increased mean and maximum life span have been reported in rats and mice maintained on diets restricted in methionine (115)(116)(117)(118)(119)(120). However, as with CR, rodents consuming low-methionine diets are smaller and eat less (10%-24% less, depending on age) than controls (117,121,122). As this continues to be a recurring issue with protein-and amino acid-restrictive diets, a few studies have attempted to show that the benefits of methionine restriction are independent of any concomitant CR.…”

Section: Dietary Restriction Alternatives To Crmentioning

confidence: 99%

Dietary Interventions to Extend Life Span and Health Span Based on Calorie Restriction

Minor

Allard

Younts

et al. 2010

The Journals of Gerontology Series A: Biological Sciences and M

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The societal impact of obesity, diabetes, and other metabolic disorders continues to rise despite increasing evidence of their negative long-term consequences on health span, longevity, and aging. Unfortunately, dietary management and exercise frequently fail as remedies, underscoring the need for the development of alternative interventions to successfully treat metabolic disorders and enhance life span and health span. Using calorie restriction (CR)-which is well known to improve both health and longevity in controlled studies-as their benchmark, gerontologists are coming closer to identifying dietary and pharmacological therapies that may be applicable to aging humans. This review covers some of the more promising interventions targeted to affect pathways implicated in the aging process as well as variations on classical CR that may be better suited to human adaptation.

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Section: Dietary Restriction Alternatives To Crmentioning

confidence: 99%

Dietary Interventions to Extend Life Span and Health Span Based on Calorie Restriction

Minor

Allard

Younts

et al. 2010

The Journals of Gerontology Series A: Biological Sciences and M

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“…The liver plays an important role in the synthesis of glutathione, which is transported by the blood to extrahepatic tissues. Richie et al (2004) observed that a Met restriction in rats greatly reduced the Cys content in liver, blood and pancreas. Compared with the control group, a Met deficiency resulted in an important reduction in glutathione content in the liver, whereas that in the blood was increased, especially in erythrocytes.…”

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confidence: 97%

“…The Cys content of empty body protein was not affected by the diet in our study, but the Cys content was higher in blood but lower in liver protein gain in TSAA2 piglets. CYS is one of the three amino acids of glutathione, which is an important antioxidant and regulator of cellular homeostasis (Richie et al, 2004). The liver plays an important role in the synthesis of glutathione, which is transported by the blood to extrahepatic tissues.…”

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confidence: 99%

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A sulfur amino acid deficiency changes the amino acid composition of body protein in piglets

Conde-Aguilera

Barea

Floc'H

et al. 2010

Animal

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Experiments carried out to determine the amino acid requirement in growing animals are often based on the premise that the amino acid composition of body protein is constant. However, there are indications that this assumption may not be correct. The objective of this study was to test the effect of feeding piglets a diet deficient or not in total sulfur amino acids (TSAA; Met 1 Cys) on nitrogen retention and amino acid composition of proteins in different body compartments. Six blocks of three pigs each were used in a combined comparative slaughter and nitrogen balance study. One piglet in each block was slaughtered at 42 days of age, whereas the other piglets received a diet deficient or not in TSAA for 19 days and were slaughtered thereafter. Two diets were formulated to provide either 0.20% Met and 0.45% TSAA (on a standardized ileal digestible basis) or 0.46% Met and 0.70% TSAA. Diets were offered approximately 25% below ad libitum intake. At slaughter, the whole animal was divided into carcass, blood, intestines, liver, and the combined head, tail, feet and other organs (HFTO), which were analyzed for nitrogen and amino acid contents. Samples of the longissimus muscle (LM) were analyzed for myosin heavy chain (MyHC) and actin contents. Nitrogen retention was 20% lower in piglets receiving the TSAA-deficient diet (P , 0.01). In these piglets, the nitrogen content in tissue gain was lower in the empty body, carcass, LM and blood (P , 0.05) or tended to be lower in HFTO (P , 0.10), but was not different in the intestines and liver. The Met content in retained protein was lower in the empty body, LM and blood (P , 0.05), and tended to be lower in the carcass (P , 0.10). The Cys content was lower in LM, but higher in blood of piglets receiving the TSAA-deficient diet (P , 0.05). Skeletal muscle appeared to be affected most by the TSAA deficiency. In LM, the Met content in retained protein was reduced by 12% and total Met retention by more than 60%. The MyHC and actin contents in LM were not affected by the TSAA content of the diet. These results show that a deficient TSAA supply affects the amino acid composition of different body proteins. This questions the use of a constant ideal amino acid profile to express dietary amino acid requirements, but also illustrates the plasticity of the animal to cope with nutritional challenges.

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“…Similar to the diet used by Richie et al, (2004) in their longevity studies, a diet with protein (casein) content , as compared to controls. Cells were seeded at confluent density onto permeable (0.4 μm pore size) Millipore PCF filter ring assemblies and after 24 hours kept on control or MR media for one week before potential difference (PD) and I scc readings were taken using Ag/AgCl electrodes in series with NaCl agar bridges and a modified Ussing chamber set-up, respectively.…”

Section: Mr Effects On Rat Small Intestine and Colonmentioning

confidence: 99%

Methionine Restriction and Modification of Epithelial Tight Junction Barrier Function and Permeability

Wang¹

2012

JEBP

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Abstract:It is well known that caloric restriction leads to an increased longevity by forestalling age-related diseases. Dietary restriction of methionine also renders similar benefits. In this review, we report studies on the effect of methionine restriction on epithelial barrier function in a renal epithelial LLC-PK 1 cell, a rodent gastrointestinal model and a clinical trial using Crohn's patients. In LLC-PK 1 cell culture, a reduction of culture medium methionine by 80% resulted in altered tight junctional claudin composition with improved epithelial barrier function as exemplified by increased transepithelial electrical resistance and decreased paracellular leak to 14 C-D-mannitol. In addition to small but significant reductions in plasma and intracellular colonocyte methionine levels, dietary methionine restriction tightens intestinal epithelium and reduces mannitol permeability in rat colonic, but not ileal tissue. Crohn's patients on methionine-restricted diet reported improved symptoms as demonstrated by a reduced CDAI index, but this diet was not able to significantly reduce the plasma methionine level in patients recruited. An increased urinary lactulose/mannitol ratio was also the result of the regimen in Crohn's patients, suggesting an increased, rather than decreased ileal leakage. Overall, our results showed that reduction in dietary intake of methionine is promising in improving at least some epithelial barriers and this may be through altering tight junctional protein compositions. Methionine restriction might result in contrasting effects depending on the tissue type and possible disease conditions, thus wider range of studies are required.

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Tissue glutathione and cysteine levels in methionine-restricted rats

Cited by 79 publications

References 34 publications

Dietary Interventions to Extend Life Span and Health Span Based on Calorie Restriction

Dietary Interventions to Extend Life Span and Health Span Based on Calorie Restriction

A sulfur amino acid deficiency changes the amino acid composition of body protein in piglets

Methionine Restriction and Modification of Epithelial Tight Junction Barrier Function and Permeability

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