Tissue Fixation Conditions for p16 Immunohistochemistry and Human Papillomavirus RNA In Situ Hybridization in Oropharyngeal Squamous Cell Carcinoma

Lewis, James S.; Barnett, Shira B.; Mannion, Kyle; Mehrad, Mitra

doi:10.1007/s12105-019-01090-6

Cited by 2 publications

(3 citation statements)

References 27 publications

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“…In one study, different tissue fixatives were reported as the culprit in false negative results of P16 immunostain in oropharyngeal SCCs, while HPV-RNA ISH results remained positive under the same conditions. 34 We compared the current results to our previous study of HPV-DNA ISH and its concordance with P16, and surgical follow-up diagnoses in 59 patients with HNSCC. 35 There was discordance between HPV-DNA ISH and P16 staining in four (7.3%) cases (three P16+/ HPV-and one P16-/HPV+).…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic utility of high‐risk human papillomavirus mRNA in situ hybridisation in squamous cell carcinoma of unknown primary in the head and neck and implementing American Society of Clinical Oncology guideline recommendations

2020

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Introduction: The American Society of Clinical Oncology (ASCO)-endorsed College of American Pathologists guideline recommends high-risk human papillomavirus (HPV) testing for metastatic squamous cell carcinoma (SCC) of lymph nodes level II/ III of unknown primary. Herein, the performance of HPV-RNA in situ hybridisation (ISH) in detection of HPV-related SCC is evaluated implementing the ASCO guideline recommendations. Methods: Eighty head and neck (HN) SCC fine needle aspirations, which utilized HPV-RNA ISH/P16, were evaluated at Johns Hopkins Hospital (2015-2018) to investigate their performance and concordance with histology. The results were compared to a prior study of 59 HNSCCs, which HPV-DNA ISH. Results: Of the 80 reviewed fine needle aspirations, 65 (50 male, 15 female) were included. The mean age was 63.2 ± 14.0 years. The most common site was neck lymph nodes (47, 72.3%). Fifty-five cases (84.6%) were accompanied by concurrent core biopsy, and 48 cases (59.4%) had surgical follow-ups. HPV-RNA ISH was positive in 44 (67.7%), and P16 was strongly positive in 46 (70.8%). The HPV-RNA ISH/ P16 concordance rate was 92.3% on cytology material. The cytology/surgical concordance rate for HPV-RNA ISH was 88.9% (16/18). There was a discordance between the results in five cases (7.7%; HPV-RNA ISH-/P16+). Conclusion: HPV-RNA ISH is a robust and reliable method for detecting HPV-related HNSCC on cytology material showing concordance rate of 92.3% between HPV-RNA ISH and P16, which is a sensitive but non-specific marker. Compared to HPV-DNA ISH, HPV-RNA ISH reproducibly identifies HPV-related HNSCC with fewer discrepancies between cytology and histology. The findings of this study are in agreement with the ASCO recommendations.

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Section: Discussionmentioning

confidence: 99%

“…Different transport media may affect the P16 staining. In one study, different tissue fixatives were reported as the culprit in false negative results of P16 immunostain in oropharyngeal SCCs, while HPV‐RNA ISH results remained positive under the same conditions 34 …”

Section: Discussionmentioning

confidence: 99%

Diagnostic utility of high‐risk human papillomavirus mRNA in situ hybridisation in squamous cell carcinoma of unknown primary in the head and neck and implementing American Society of Clinical Oncology guideline recommendations

2020

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“…Récemment, Lewis et al ont montré qu'un retard de fixation pouvait être responsable d'une perte artéfactuelle de l'immunomarquage anti-p16 par autolyse tissulaire. Dans ce travail, de façon intéressante, l'hybridation in situ ciblant l'ARN restait positive quelque soient les conditions de fixation [32]. Enfin, des modifications épigénétiques pourraient être responsables de l'extinction secondaire de la protéine p16, par hyperméthylation du gène codant la protéine p16 [33].…”

Section: Discussionunclassified

Étude de la prévalence du papillomavirus (HPV) dans les cancers des voies aéro-digestives sur une cohorte unicentrique française de 372 patients

Outh-Gauer

Mandavit

Lépine

et al. 2020

Annales de Pathologie

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Tissue Fixation Conditions for p16 Immunohistochemistry and Human Papillomavirus RNA In Situ Hybridization in Oropharyngeal Squamous Cell Carcinoma

Cited by 2 publications

References 27 publications

Diagnostic utility of high‐risk human papillomavirus mRNA in situ hybridisation in squamous cell carcinoma of unknown primary in the head and neck and implementing American Society of Clinical Oncology guideline recommendations

Diagnostic utility of high‐risk human papillomavirus mRNA in situ hybridisation in squamous cell carcinoma of unknown primary in the head and neck and implementing American Society of Clinical Oncology guideline recommendations

Étude de la prévalence du papillomavirus (HPV) dans les cancers des voies aéro-digestives sur une cohorte unicentrique française de 372 patients

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