Tissue Factor Expression in Non-small Cell Lung Cancer: Relationship with Vascular Endothelial Growth Factor Expression, Microvascular Density, and K-ras Mutation

Régina, Sandra; Rollin, Jérôme; Bléchet, Claire; Iochmann, Sophie; Reverdiau, Pascale; Gruel, Yves

doi:10.1097/jto.0b013e31817c1b21

Cited by 64 publications

(57 citation statements)

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“…In NSCLC, we recently demonstrated that tumor TF gene (F3) expression varied between patients but was significantly higher in patients with advanced stages of cancer (4 ). Moreover, our study also showed a relationship between overexpression of tumor TF and the presence of a mutation of codon 12 of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS).…”

confidence: 50%

“…TF is frequently upregulated in cancer cells, particularly in the later stages of disease progression (26 ), and we recently reported that TF gene expression was higher in advanced stages of NSCLC and when codon 12 of the KRAS gene was mutated (4 ). Both TF expression and TF procoagulant and proangiogenic activity have previously been shown to be regulated by mutations of the KRAS oncogene and TP53 tumor suppressor gene in human colorectal cell lines (5 ).…”

Section: Tf Expression and Oncogene Events In Nsclcmentioning

confidence: 99%

“…Lung cancers are almost exclusively carcinomas that originate in the epithelia of the trachea, bronchi, or lungs. Several histological types have been identified and they are divided into 2 groups, small cell lung cancer (SCLC) 4 (20%) and non-SCLC (NSCLC) (80%).…”

confidence: 99%

“…The results were expressed as the number of heparanase mRNA copies for 10 8 copies of 18S RNA. Tumor TF gene (F3) expression was analyzed by real-time PCR as previously described (4 ).…”

Section: Rna Extraction and Real-time Pcr For Heparanase And Tfmentioning

confidence: 99%

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Increased Tissue Factor Expression Is Associated with Reduced Survival in Non–Small Cell Lung Cancer and with Mutations of TP53 and PTEN

et al. 2009

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Background: Tissue factor (TF), the main initiator of blood coagulation, is also a signaling protein that regulates cancer progression. TF synthesis was recently shown to be affected by tumor suppressor genes (TSGs) in tumor cell lines. We therefore studied TF gene (F3) expression and the status of genes coding for tumor protein p53 (TP53), phosphatase and tensin homolog (PTEN), and serine/threonine kinase 11 (STK11) in non–small cell lung cancer (NSCLC). Heparanase (HPSE) gene expression was also measured because this endo-β-D-glucuronidase was recently shown to enhance TF gene expression. Methods: TF and heparanase mRNA expression was measured by real-time PCR in 53 NSCLC tumors. Exons 5–8 of TP53 were sequenced from genomic DNA. Mutations of PTEN and STK11 were screened by multiplex ligation-dependent probe amplification. Results: TF mRNA levels were significantly higher in T3–T4 tumors (P = 0.04) and in stages III–IV of NSCLC (P = 0.03). Mutations of TP53, STK11, and PTEN were identified in 20 (37.7%), 21 (39%), and 20 (37.7%) of tumors, respectively. TF expression was higher in mutated TP53 (TP53Mut) (P = 0.02) and PTENMut (P = 0.03) samples. Moreover, TF mRNA increased from 2700 copies (no mutation) to 11 6415 when 3 TSG were mutated. Heparanase gene expression did not differ according to TF gene (F3) expression or TSG mutation. The median survival time was shorter in patients with tumor TF mRNA levels above median values (relative risk 2.2; P = 0.03, multivariate analysis) and when TP53 was mutated (relative risk 1.8; P = 0.02). Conclusions: These results provide clear evidence that combined oncogene events affecting TSG dramatically increase TF gene expression in lung tumors. Moreover, this study suggests that TF gene expression could be used as a prognostic marker in NSCLC. .

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confidence: 50%

Section: Tf Expression and Oncogene Events In Nsclcmentioning

confidence: 99%

“…The results were expressed as the number of heparanase mRNA copies for 10 8 copies of 18S RNA. Tumor TF gene (F3) expression was analyzed by real-time PCR as previously described (4 ).…”

Section: Rna Extraction and Real-time Pcr For Heparanase And Tfmentioning

confidence: 99%

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Increased Tissue Factor Expression Is Associated with Reduced Survival in Non–Small Cell Lung Cancer and with Mutations of TP53 and PTEN

et al. 2009

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“…12 Pada KNF belum diketahui mutasi onkogen, sedangkan pada kanker kolorektal 10 dan karsinoma paru 12 diketahui k-ras mutasi sehingga ekspresi TF akan semakin meningkat.…”

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Perbedaan Ekspresi Vascular Endothelial Growth Factor dan Ekspresi Tissue Factor Berdasarkan Respons Terapi Kemoradiasi Cisplatin pada Penderita Karsinoma Nasofaring Stadium lanjut

Hendarsih¹,

Oehadian²,

Sumantri³

et al. 2015

mkb

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AbstrakVascular endothelial growth factor (VEGF) merupakan faktor angiogenik yang berperan dalam angiogenesis tumor. Tissue factor (TF) merupakan inisiator utama pembekuan darah dan merangsang protein yang mengatur angiogenesis. Penelitian bertujuan mengetahui perbedaan ekspresi VEGF dengan ekspresi TF pada penderita KNF stadium lanjut berdasarkan respons terapi kemoradiasi. Dilakukan penelitian kohort prospektif pada penderita KNF stadium III, IVa, dan IVb berdasarkan AJCC edisi ke-7 tahun 2010. Ekspresi TF dan VEGF diperiksa dengan imunohistokimia dan respons kemoradioterapi dievaluasi dengan memakai Response Evaluation Criteria in Solid Tumours (RECIST) revisi versi 1.1 tahun 2009. Analisis statistik yang digunakan adalah Uji eksak Fisher.Selama penelitian Oktober 2012-Oktober 2013 didapatkan 35 penderita KNF yang memenuhi kriteria inklusi dan 5 orang dikeluarkan dari penelitian. Pada kelompok respons didapatkan 17 dari 23 penderita ekspresi VEGF ≥ 25%; 6 dari 23 penderita ekspresi VEGF <25%; 16 dari 23 penderita ekspresi TF ≥ 33%; 7 dari 23 penderita ekspresi TF <33% (p=1,000) serta pada kelompok tidak respons 5 dari 27 penderita ekspresi VEGF ≥25%; 2 dari 7 penderita ekspresi VEGF <25%; 4 dari 7 penderita ekspresi TF ≥ 33%; 3 dari 7 penderita ekspresi TF <33% (p=0,657). Simpulan, tidak terdapat perbedaan ekspresi VEGF dengan ekspresi TF pada penderita KNF stadium lanjut berdasarkan respons terapi kemoradiasi. [MKB. 2015;47 (1)

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A case of disseminated intravascular coagulation following tumour lysis syndrome due to small cell carcinoma of the lung

Takahashi

Takehara

Tani

et al. 2023

Respirology Case Reports

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A 64‐year‐old man was diagnosed with small cell lung cancer (SCLC) with multiple bone and liver metastases and bone marrow metastases. Spontaneous tumour lysis syndrome (TLS) was observed before starting chemotherapy with carboplatin, etoposide, and atezolizumab. The tumour further collapsed, and the patient developed disseminated intravascular coagulation (DIC) on day 4 of chemotherapy. The patient was successfully treated with intravenous hydration and rasburicase for TLS and subcutaneous unfractionated heparin for DIC. A large amount of tissue factor may be released in TLS, which could induce DIC. However, to the best of our knowledge, this is the first report of DIC following TLS in a case of SCLC. DIC following TLS in SCLC is a rare but life‐threatening oncologic complication. Therefore, clinicians should be aware of this possibility when treating patients with advanced SCLC.

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Tissue Factor Expression in Non-small Cell Lung Cancer: Relationship with Vascular Endothelial Growth Factor Expression, Microvascular Density, and K-ras Mutation

Abstract: These results suggest that high TF expression in lung tumors may result from K-ras mutation and contribute to NSCLC progression, probably via mechanisms other than angiogenesis.

Cited by 64 publications

References 48 publications

Increased Tissue Factor Expression Is Associated with Reduced Survival in Non–Small Cell Lung Cancer and with Mutations of TP53 and PTEN

Increased Tissue Factor Expression Is Associated with Reduced Survival in Non–Small Cell Lung Cancer and with Mutations of TP53 and PTEN

Perbedaan Ekspresi Vascular Endothelial Growth Factor dan Ekspresi Tissue Factor Berdasarkan Respons Terapi Kemoradiasi Cisplatin pada Penderita Karsinoma Nasofaring Stadium lanjut

A case of disseminated intravascular coagulation following tumour lysis syndrome due to small cell carcinoma of the lung

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