Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

Kim, Suran; Min, Sungjin; Choi, Yi Sun; Jo, Sung‐Hyun; Jung, Jae Hun; Han, Kyusun; Kim, Jin; An, Soohwan; Ji, Yong Woo; Kim, Yun‐Gon; Cho, Seung‐Woo

doi:10.1038/s41467-022-29279-4

Cited by 141 publications

(108 citation statements)

References 103 publications

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“…Despite the outstanding bioactivity, the isolation from tumor tissue hinders its translation into clinical applications. Kim et al [ 55 ] has demonstrated that the extracellular matrix hydrogels derived from decellularized gastrointestinal tissues are effective alternatives to the current gold standard, Matrigel. This might be a solution to avoid Matrigel's prohibition in clinical translation, which needs further experiment.…”

Section: Resultsmentioning

confidence: 99%

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

Xia

Li²,

Wang³

et al. 2023

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

Xia

Li²,

Wang³

et al. 2023

Bioactive Materials

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“…The copyright holder for this preprint this version posted August 22, 2022. ; https://doi.org/10.1101/2022.08.20.504600 doi: bioRxiv preprint within the community to generate biomatrix alternatives to replace commonly used Matrigel™ since it cannot be easily standardized [47,48]. Many of these alternative bio-matrices are viscous hydrogels or hydrogel precursors therefore automated tissue manufacturing methods compatible with these more viscous materials will likely be important as the use of these alternative gels increases [49]. We anticipate our workflow is likely compatible with many of these synthetic gels, particularly those with a thermal gelation step, however some parameter optimization may be necessary following similar steps to those described here.…”

Section: Automated Dispensing Sequences Support the Growth Of Organoi...mentioning

confidence: 99%

An automation workflow for high-throughput manufacturing and analysis of scaffold-supported 3D tissue arrays

Cao

Cadavid

et al. 2022

Preprint

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The success rate of bringing novel cancer therapies to the clinic remains extremely low due to the lack of relevant pre-clinical culture models that capture the complexity of human tumours. Patient-derived organoids have emerged as a useful tool to model patient and tumour heterogeneity to begin addressing this need. Scaling these complex culture models while enabling stratified analysis of different cellular sub-populations remains a challenge, however. One strategy to enable higher throughput organoid cultures that also enables easy image-based analysis is the Scaffold-supported Platform for Organoid-based Tissues (SPOT) platform. SPOT allows the generation of flat, thin and dimensionally-defined microtissues in both 96- and 384-well plate footprints and is compatible with tumour organoid culture and downstream image-based readouts. SPOT manufacturing is currently a manual process however, limiting the use of SPOT to perform larger-scale screening. In this study, we integrate and optimize an automation approach to generate tumour-mimetic 3D engineered microtissues in SPOT using a liquid handler, and show comparable within-sample and between-sample variation as the standard manual manufacturing process. Furthermore, we develop a liquid handler-supported whole-cell extraction protocol and as a proof-of-value demonstration, we generate 3D complex tissues containing different proportions of tumour and stromal cells and perform single-cell-based end-point analysis to demonstrate the impact of co-culture on the tumour cell population specifically. We also demonstrate we can incorporate primary patient-derived organoids into the pipeline to capture patient-level tumour heterogeneity. We envision that this automated workflow integrated with 96/384-SPOT and multiple cell types and patient-derived organoid models will provide opportunities for future applications in high-throughput screening for novel personalized therapeutic targets. This pipeline also allows the user to assess dynamic cell responses using high-content longitudinal imaging or downstream single-cell-based analyses.

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“…Several studies have developed other scaffold-based technologies or designed novel chemical materials as potential alternatives to the widely used Matrigel. For example, Kim et al [127] demonstrated that gastrointestinal tissue-derived extracellular matrix hydrogels were effective substitutes for Matrigel in gastrointestinal organoid culture. Gastrointestinal extracellular matrix hydrogels enabled long-term subculturing and transplantation of organoids by providing gastrointestinal tissue-mimetic microenvironments.…”

Section: Challenges Of Organoidsmentioning

confidence: 99%

Urological cancer organoids, patients' avatars for precision medicine: past, present and future

et al. 2022

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Urological cancers are common malignant cancers worldwide, with annually increasing morbidity and mortality rates. For decades, two-dimensional cell cultures and animal models have been widely used to study the development and underlying molecular mechanisms of urological cancers. However, they either fail to reflect cancer heterogeneity or are time-consuming and labour-intensive. The recent emergence of a three-dimensional culture model called organoid has the potential to overcome the shortcomings of traditional models. For example, organoids can recapitulate the histopathological and molecular diversity of original cancer and reflect the interaction between cancer and surrounding cells or stroma by simulating tumour microenvironments. Emerging evidence suggests that urine-derived organoids can be generated, which could be a novel non-invasive liquid biopsy method that provides new ideas for clinical precision therapy. However, the current research on organoids has encountered some bottlenecks, such as the lack of a standard culture process, the need to optimize the culture medium and the inability to completely simulate the immune system in vivo. Nonetheless, cell co-culture and organoid-on-a-chip have significant potential to solve these problems. In this review, the latest applications of organoids in drug screening, cancer origin investigation and combined single-cell sequencing are illustrated. Furthermore, the development and application of organoids in urological cancers and their challenges are summarised.

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Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

Cited by 141 publications

References 103 publications

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

An automation workflow for high-throughput manufacturing and analysis of scaffold-supported 3D tissue arrays

Urological cancer organoids, patients' avatars for precision medicine: past, present and future

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