Tissue Expression, Serum and Salivary Levels of IL-10 in Patients with Head and Neck Squamous Cell Carcinoma

Abstract

Background: Head and neck SCC is a common cancer related to various factors. IL-10, a pleiotropic cytokine produced by macrophages, T-helper-2 cells, and B lymphocytes, is thought to play a potential pathogenetic or therapeutic role in a number of human conditions, such as inflammation, autoimmunity and cancer. The present study was designed to evaluate the relation between tissue expression, serum and salivary levels of IL-10 in head and neck squamous cell carcinomas (HNSCCs) and their correlation with clinicopathologic features. Materials and Methods: Samples were collected from 30 patients with HNSCCs and 24 healthy volunteers. IHC analysis was used to examine the tissue expression and ELISA was employed to measure serum and salivary levels. Results: Our study showed tissue expression of IL-10 to be significantily higher in patients (P: 0.001), but there was no relation between tissue expression , serum and salivary levels of the marker (P>0.05). Also except for a positive correlation between tissue expression of IL-10 and stage (P: 0.044), there was no relation between this marker and clinicopathologic features. There was no correlation between serum and salivary levels in either patients or controls. Conclusions: It seems there is no correlation between level of IL-10 in serum and saliva and this marker in saliva and serum does not reflect tissue expression.

Salivary Immunosuppressive Cytokines IL-10 and IL-13 Are Significantly Elevated in Oral Squamous Cell Carcinoma Patients

“…These results are consistent with other studies regarding IL-10 status in OSCC, although these studies have analyzed the IL-10 protein and mRNA levels in OSCC tumor tissues (35,36). Moreover, Hamzavi et al studied and compared the tissue expression, serum, and salivary levels of IL-10 and found no correlation among them in either controls or OSCC subjects (30), which reflects different levels of regulation of this cytokine in different compartments of the body. Alhamarneh et al found significantly higher serum levels of IL-10 in advanced stages of head and neck squamous cell carcinoma except oral cavity tumors (37).…”

“…A study conducted by Kaskas et al showed contradictory results and reported significantly lower serum levels of IL-13 in head and neck squamous cell carcinoma as compared to normal healthy controls (29). It has also been shown in other studies as well, that the alterations in cytokine phenotypes in the patients' oral lesions are not necessarily reflected in the peripheral blood (serum/plasma; 30,31). This discrepancy could be explained in several ways.…”

Associations among Inflammatory Biomarkers in the Circulating, Plasmatic, Salivary and Intraluminal Anatomical Compartments in Apparently Healthy Preschool Children from the Western Highlands of Guatemala

“…In a survey of the salivary-cytokine literature, we find that the majority involve local conditions arising close to the salivary glands, such as oral inflammation [30,31] or head neck or oral, cancer [32,33]. Our observed dissociation of the cytokine response between the circulating pattern and that in the saliva, moreover, is neither unprecedented nor implausible.…”

Interleukin-10 Superfamily and Cancer

“…Additionally, it was also observed that in patients with hepatocellular carcinoma (HCC), IL-10 contributed to the Treg-mediated suppression of the cytotoxic activities and IFN-γ production by γδ T cells (Yi et al, 2013). Finally, conflicting results were obtained regarding the association between serum concentrations of IL-10 and head and neck squamous cell carcinoma (Melinceanu et al, 2011;Mojtahedi et al, 2012;Hamzavi et al, 2013) and HCC (Jang et al, 2012;Welling et al, 2012).…”