Tissue expression of β-catenin and E- and N-cadherins in chronic hepatitis C and hepatocellular carcinoma

Kasprzak, Aldona; Rogacki, Karol; Adamek, Agnieszka; Sterzyńska, Karolina; Przybyszewska, Wiesława; Seraszek‐Jaros, Agnieszka; Helak-Łapaj, Celina; Pyda, Przemysław

doi:10.5114/aoms.2017.65272

Cited by 17 publications

(27 citation statements)

References 32 publications

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“…Hepatitis C virus (HCV) infection is a major health problem all over the world. It can cause chronic hepatitis and liver cirrhosis and represents the main risk factor of primary hepatocellular carcinoma [1]. It is estimated to chronically affect ~3% of the world's population (~170 million people), with more than one million new cases annually [1].…”

Section: Introductionmentioning

confidence: 99%

“…It can cause chronic hepatitis and liver cirrhosis and represents the main risk factor of primary hepatocellular carcinoma [1]. It is estimated to chronically affect ~3% of the world's population (~170 million people), with more than one million new cases annually [1]. There is great variability in HCV prevalence rate from one country to another and even within the same country from one region to another [2].…”

Section: Introductionmentioning

confidence: 99%

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Perinatal transmission of hepatitis C virus: an update

El‐Shabrawi¹,

Kamal²,

Mogahed³

et al. 2020

aoms

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Infection with hepatitis C virus (HCV) is a major health problem worldwide. A large proportion of perinatal HCV infections are silent and may present later in adulthood with long-term complications. HCV has no effective immune prophylaxis and hence appropriate follow-up of all infants born to HCV-infected mothers is necessary. Universal antenatal screening for HCV is largely debatable. Intrauterine and partum transmission of HCV are both possible and higher rates are associated with a high maternal serum viral load (> 10 6 copies per milliliter), concomitant HIV infection, prolonged or difficult delivery, and invasive fetal monitoring during delivery. Infection during pregnancy and infancy needs to be investigated more in order to design management strategies for perinatal transmission of HCV most effectively. The recently approved new-generation, oral, direct-acting antiviral drugs may open a new era in HCV therapy for pregnant women and infected infants if proved to be safe during conception and infancy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Perinatal transmission of hepatitis C virus: an update

El‐Shabrawi¹,

Kamal²,

Mogahed³

et al. 2020

aoms

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“…Expression of adhesive protein genes such as e-cadherin and n-cadherin was higher under the in uence of GO nano lm and nfGO + CELE, especially in HepG2 cells, but most important was a reduction in βcatenin expression in these cells [11,54,59]. Our results, like Mu et al, showed that cells increase the expression of E-cadherin on nfGO, moreover, in view of the fact that the weakening of tight connections (E-cadherin expression) and an increase in exible cell-cell contacts (N-cadherin expression) is characteristic of HCC [21].…”

Section: Discussionmentioning

confidence: 99%

“…Our results, like Mu et al, showed that cells increase the expression of E-cadherin on nfGO, moreover, in view of the fact that the weakening of tight connections (E-cadherin expression) and an increase in exible cell-cell contacts (N-cadherin expression) is characteristic of HCC [21]. Thus, the increase in expression of two types of intercellular junctions suggests that the experimental factors used (nfGO and CELE) did not increase the invasion of HepG2 cells [21,59,60]. These results are also con rmed by the observation of cell morphology, which re ects the cell-ECM and cell-cell relationship and also indicates material biocompatibility [32,61,62,63].…”

Section: Discussionmentioning

confidence: 99%

Graphene Oxide Nanofilm and Chicken Embryo Extract Decrease the Invasiveness of HepG2 Liver Cancer Cells.

Sosnowska

Kutwin

Strojny

et al. 2020

Preprint

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Background: The extracellular matrix (ECM) is a mosaic of various structural and functional proteins that cooperate with the cell, regulate adhesion, and consequently manage its further fate. Liver destruction is accompanied by a disruption of the physicochemical properties of the ECM which deregulates the cell-ECM interaction and can lead to uncontrolled proliferation and neoplastic transformation of cells. Therefore, it can be assumed that ECM modification and restoration of its characteristics for healthy tissue may counteract uncontrolled cell proliferation. The purpose of the presented research model was to optimise the physical characteristics of ECM by introducing a graphene oxide plane/nanofilm (nfGO) and enriching the cell environment with potentially missing proteins by adding a functional protein cocktail (chicken embryo liver extract) and determine the impact of these factors on cell-ECM cooperation and its consequences on adhesion, proliferation, and cell phase, which are factors of the invasiveness of cancer cells.Results: Experiments were performed with non-cancer HS-5 cells and liver cancer cells HepG2 and C3A. The cells were divided into four groups; (1) control, (2) cultured on GO nanofilm, (3) cultured with the addition of chicken embryo liver extract (CELE) to the medium and (4) cultured on the GO nanofilm with the addition of CELE. CELE contained 1735 proteins; the top 57 of these proteins have been presented. The use of GO nanofilm as well as CELE and nfGO + CELE reduced the proliferation of HepG2 cancer cells to the greatest extent; this is in contrast to non-cancer cells and also to C3A cancer cells. Furthermore, the combined use of the CELE protein cocktail and GO substrate effectively resulted in a decrease in the population of HepG2 cells in the G0/G1 phase and an increase of the population in G2/M. Molecular analysis of HepG2 cancer cells also showed an increase in the expression of genes responsible for adhesion such as fak (focal adhesion kinase), e-cadherin, and n-cadherin and a decrease in β-catenin, which is considered a proto-oncogen. Conclusions: Studies have shown that both the GO surface structure on which the cells are grown as well as the presence of a multi-component natural cocktail of regulatory proteins, can modify the expression of integrins, increase adhesion and, as a consequence, proliferation and the cell cycle - entering the resting phase. For the first time, it has been documented that hepatic cancer cells of the HepG2 line under the influence of stimuli derived from mimic ECM (graphene oxide) in interaction with a unique protein complex derived from chicken liver embryo decreased of the invasiveness of cancer cells.

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“…HCV proteins affect in a double manner expression of E-cadherin, including modulation of the Wnt pathway and reduction of E-cadherin expression at the transcriptional level. Alterations in cellular locations of β-catenin and E-cadherin in chronic HCV and HCC pointed to structural disturbances in intercellular junctions in livers and presence of the transcriptionally inactive form of β-catenin [55,56] . Promoter hypermethylation of Wnt inhibitors was discovered in HCV-induced multistep hepatocarcinogenesis [57] , and the reduced expression of E-cadherin in long-lasting chronic HCV might represent an early indicator of the epithelial-mesenchymal transition [58,59] .…”

Section: Hcv Provoked Wnt Signalingmentioning

confidence: 99%

Oncogenic Wnt3a: a promising specific biomarker in hepatocellular carcinoma

Yao¹,

Miao²,

Zheng³

et al. 2018

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Hepatocellular carcinoma (HCC) is still one of the most common and rapidly fatal malignancies worldwide with a multi-factorial, multi-step, complex process, and poor prognosis. Early discovery and effective therapy of HCC are of utmost importance. Recent studies demonstrated that Wnt/β-catenin pathway play important roles in occurrence and development of HCC including hepatocytes malignant transformation, metastasis, chemoresistance and liver cancer stem cells. Oncogenic wingless-type MMTV integration site family member 3a (Wnt3a) signaling is a promising biomarker in diagnosis and prognosis for HCC. This review presents current data on mechanisms of hepatocarcinogenesis involving participation of the Wnt canonical pathway, and focuses on the Wnt3a expression in HCC progression and its clinical application.

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Tissue expression of β-catenin and E- and N-cadherins in chronic hepatitis C and hepatocellular carcinoma

Cited by 17 publications

References 32 publications

Perinatal transmission of hepatitis C virus: an update

Perinatal transmission of hepatitis C virus: an update

Graphene Oxide Nanofilm and Chicken Embryo Extract Decrease the Invasiveness of HepG2 Liver Cancer Cells.

Oncogenic Wnt3a: a promising specific biomarker in hepatocellular carcinoma

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