Tissue engineering of human oral mucosa on different scaffolds: in vitro experiments as a basis for clinical applications

Kriegebaum, Ulrike; Mildenberger, Michael; Mueller-Richter, Urs D.A.; Klammert, Uwe; Kuebler, Alexander C.; Reuther, T.

doi:10.1016/j.oooo.2011.10.019

Cited by 20 publications

(15 citation statements)

References 28 publications

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“…In this work, we have performed a sequential study of epithelial layer development of a human AOM model by evaluating key basement membrane proteins and intercellular junctions. Most of the works focused on the study of AOM have used partial and non‐autologous oral mucosa models to identify epithelial patterns such as DG3, laminin and others . On the one hand, our results confirmed the epithelial phenotype of the bioengineered epithelial layer as determined by the positive expression of pancytokeratin in all AOM samples as we previously demonstrated .…”

Section: Discussionsupporting

confidence: 86%

Sequential keratinocytic differentiation and maturation in a three‐dimensional model of human artificial oral mucosa

Viñuela-Prieto

Sánchez-Quevedo

Alfonso

et al. 2014

J of Periodontal Research

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Section: Discussionsupporting

confidence: 86%

Sequential keratinocytic differentiation and maturation in a three‐dimensional model of human artificial oral mucosa

Viñuela-Prieto

Sánchez-Quevedo

Alfonso

et al. 2014

J of Periodontal Research

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“…42 In addition, the TF membrane was described as very smooth and flexible, with deformations of the surface in places with the greatest cell growth. 42 In our study, the scaffolds remained stable during the entire in vitro culture time of 20 days. Thus, all four scaffolds assessed in this study would meet the criteria for an optimal 3D membrane in a clinical setting, as they would preserve the architecture of the tissue during the regeneration process.…”

Section: Collagen IV Formation In Teomsmentioning

confidence: 99%

Full‐thickness tissue engineered oral mucosa for genitourinary reconstruction: A comparison of different collagen‐based biodegradable membranes

et al. 2020

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Tissue engineering is a method of growing importance regarding clinical application in the genitourinary region. One of the key factors in successfully development of an artificially tissue engineered mucosa equivalent (TEOM) is the optimal choice of the scaffold. Collagen scaffolds are regarded as gold standard in dermal tissue reconstruction. Four distinct collagen scaffolds were evaluated for the ability to support the development of an organotypical tissue architecture. TEOMs were established by seeding cocultures of primary oral epithelial cells and fibroblasts on four distinct collagen membranes. Cell viability was assessed by MTT-assay. The 3D architecture and functionality of the tissue engineered oral mucosa equivalents were evaluated by confocal laser-scanning microscopy and immunostaining. Cell viability was reduced on the TissuFoil E ® membrane. A multi-stratified epithelial layer was established on all four materials, however the TEOMs on the Bio-Gide ® scaffold showed the best fibroblast differentiation, secretion of tenascin and fibroblast migration into the membrane. The TEOMs generated on Bio-Gide ® scaffold exhibited the optimal cellular organization into a cellular 3D network. Thus, the Bio-Gide ® scaffold is a suitable matrix for engineering of mucosa substitutes in vitro. K E Y W O R D S biodegradable scaffolds, coculture, primary oral epithelial cells, primary oral fibroblasts, tissue engineered mucosa equivalents 1 | INTRODUCTION Tissue engineering is a relatively new emerging technology in the field of regenerative medicine. It developed because of the growing needs of plastic and reconstructive surgery to restore form and function in a subset of patients with tissue damage due to preexisting or neoplastic disease, trauma or congenital anomalies on one hand, and from the need to improve aesthetic appearance after surgeries on the other. 1 Thus, it is not surprising that skin was one of the first engineered tissues to be used in the clinical setting. 1 Humby first described the successful use of buccal mucosa for reconstruction surgery in males with hypospadias in

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“…[1213] Recent advances in cell culturing have developed protocols to growth keratinocytes, necessary for skin and mucosa, without the use of serum, irradiated feeder layers, and pituitary extract; thus paving the way for their use in clinical applications. [14] Numerous types of scaffolds made of synthetic or natural materials such as decellularized, freeze-dried dermis (AlloDerm ® ) has been used for years with predictable results,[23515] for simple soft tissue reconstruction. In contrast, success has not been ideal for reconstruction of complex soft tissue structures composed of epithelium, dermis, and muscle that require a robust vascular supply, such as the lips.…”

Section: Introduction/basic Principles Of Tissue Engineeringmentioning

confidence: 99%

Soft tissue engineering in craniomaxillofacial surgery

Kim¹,

Fasi²,

Feinberg³

2014

Ann Maxillofac Surg

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Craniofacial soft tissue reconstruction may be required following trauma, tumor resection, and to repair congenital deformities. Recent advances in the field of tissue engineering have significantly widened the reconstructive armamentarium of the surgeon. The successful identification and combination of tissue engineering, scaffold, progenitor cells, and physiologic signaling molecules has enabled the surgeon to design, recreate the missing tissue in its near natural form. This has resolved the issues like graft rejection, wound dehiscence, or poor vascularity. Successfully reconstructed tissue through soft tissue engineering protocols would help surgeon to restore the form and function of the lost tissue in its originality. This manuscript intends to provide a glimpse of the basic principle of tissue engineering, contemporary, and future direction of this field as applied to craniofacial surgery.

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Tissue engineering of human oral mucosa on different scaffolds: in vitro experiments as a basis for clinical applications

Cited by 20 publications

References 28 publications

Sequential keratinocytic differentiation and maturation in a three‐dimensional model of human artificial oral mucosa

Sequential keratinocytic differentiation and maturation in a three‐dimensional model of human artificial oral mucosa

Full‐thickness tissue engineered oral mucosa for genitourinary reconstruction: A comparison of different collagen‐based biodegradable membranes

Soft tissue engineering in craniomaxillofacial surgery

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