Tissue Engineering of Corneal Stromal Layer with Dermal Fibroblasts: Phenotypic and Functional Switch of Differentiated Cells in Cornea

Zhang, Yan Qing; Zhang, Wen Jie; Liu, Wei; Hu, Xiao Jie; Zhou, Guang; Cui, Lei; Cao, Yilin

doi:10.1089/tea.2007.0200

Cited by 21 publications

(6 citation statements)

References 21 publications

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“…Thus, alternative cell sources should be tested. In our previous study, we reported that dermal fibroblasts could switch their phenotype to keratocytes in the cornea stromal environment [ 34 ]. Using such cells should therefore be investigated in the future.…”

Section: Discussionmentioning

confidence: 99%

Corneal Stroma Regeneration with Acellular Corneal Stroma Sheets and Keratocytes in a Rabbit Model

et al. 2015

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Acellular corneal stroma matrix has been used for corneal stroma engineering. However, because of its compact tissue structure, regrowth of keratocytes into the scaffold is difficult. Previously, we developed a sandwich model for cartilage engineering using acellular cartilage sheets. In the present study, we tested this model for corneal stroma regeneration using acellular porcine corneal stroma (APCS) sheets and keratocytes. Porcine corneas were decellularized by NaCl treatment, and the APCS was cut into 20-μm-thick sheets. A rabbit corneal stroma defect model was created by lamellar keratoplasty and repaired by transplantation of five pieces of APCS sheets with keratocytes. Six months after transplantation, transparent corneas were present in the experimental group, which were confirmed by anterior segment optical coherence tomography examination and transmittance examination. The biomechanical properties in the experimental group were similar to those of normal cornea. Histological analyses showed an even distribution of keratocytes and well-oriented matrix in the stroma layer in the experimental group. Together, these results demonstrated that the sandwich model using acellular corneal stroma sheets and keratocytes could be potentially useful for corneal stroma regeneration.

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Section: Discussionmentioning

confidence: 99%

Corneal Stroma Regeneration with Acellular Corneal Stroma Sheets and Keratocytes in a Rabbit Model

et al. 2015

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“…[6][7][8] In our previous study, DFs were observed to undergo a phenotypic and functional switch to cornea stromal-like cells when implanted in a corneal stromal layer without induction. 9 These findings suggest that DFs may possess the capability of transdifferentiating into other cell types when offered with a suitable inducing agent or microenvironment.…”

Section: Introductionmentioning

confidence: 92%

Chondrogenic Transdifferentiation of Human Dermal Fibroblasts Stimulated with Cartilage-Derived Morphogenetic Protein 1

Yin

Chen²,

Wang

et al. 2010

Tissue Engineering Part A

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This study aimed to investigate the chondrogenic transdifferentiation potential of human dermal fibroblasts (DFs) by stimulation with cartilage-derived morphogenetic protein 1 (CDMP1). Using CDMP1 (100 ng/mL) we induced human DFs at passage 5 in both monolayer and micromass culture. Chondrogenic-specific markers were detected via immunochemistry, reverse transcription-polymerase chain reaction, and Western blot analysis in the collected specimens. The expression profile of adhesion molecules including integrin alpha5, beta1, and N-cadherin of DFs accompanying with chondrogenesis was further investigated. After 7 days of induction in monolayer culture, DFs acquired the polygonal chondrocyte-like shape with positive expression of chondrogenic-specific markers. Such a phenotypic transition of DFs was lost at 14 days. However, in micromass culture the chondrogenic transdifferentiation of DFs can be maintained even at 14 days. No chondrogenesis was detected in DFs without CDMP1 treatment under both culture conditions. By neutralization assay with blocking antibodies, it was further revealed that integrin alpha5 expression was in direct proportion to the degree of chondrogenic differentiation. Based on our findings, it can be ascertained that DFs are capable of transdifferentiating into a chondrogenic lineage by stimulation with CDMP1 in vitro. The integrin alpha5 mediating cell-cell and cell-matrix interactions is required for maintaining the chondrogenic phenotype of DFs.

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“…23 For example, DFs were successfully used as seed cells to regenerate corneal stromal layer and tendon via tissue engineering technique. [24][25][26] In this study, DFs were used as seed cells to regenerate spinal dura tissue, which was inspired by the fact that spinal dura was composed of highly oriented collagen fibers as matrix lined with small amount of fibroblasts. It was then shown that histological structure of the reconstructed tissue of the experimental group bore great similarity to that of normal one.…”

Section: Discussionmentioning

confidence: 99%

Reconstructing spinal dura-like tissue using electrospun poly(lactide-co-glycolide) membranes and dermal fibroblasts to seamlessly repair spinal dural defects in goats

Li¹,

Yin²,

Liao

et al. 2015

J Biomater Appl

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Many neuro- and spinal surgeries involving access to the underlying nervous tissue will cause defect of spinal dural mater, further resulting in cerebrospinal fluid leakage. The current work was thus aimed to develop a package which included two layers of novel electrospun membranes, dermal fibroblasts and mussel adhesive protein for repairing spinal dural defect. The inner layer is electrospun fibrous poly(lactide-co-glycolide) membrane with oriented microstructure (O-poly(lactide-co-glycolide)), which was used as a substrate to anchor dermal fibroblasts as seed cells to reconstitute dura-like tissue via tissue engineering technique. The outer layer is chitosan-coated electrospun nonwoven poly(lactide-co-glycolide) membrane (poly(lactide-co-glycolide)-chitosan). During surgery, the inner reconstituted tissue layer was first used to directly cover dura defects, while the outer layer was placed onwards with its marginal area tightly immobilized to the surrounding normal spinal dura aided by mussel adhesive protein. Efficacy of the current design was verified in goats with spinal dural defects (0.6 cm × 0.5 cm) in lumbar. It was shown that seamless and quick sealing of the defect area with the implants was realized by mussel adhesive protein. Guided tissue growth and regeneration in the defects of goats were observed when they were repaired by the current package. Effective cerebrospinal fluid containment and anti-adhesion of the regenerated tissue to the surrounding tissue could be achieved in the current animal model. Hence, it could be ascertained that the current package could be a favorite choice for surgeries involving spinal dural defects.

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Tissue Engineering of Corneal Stromal Layer with Dermal Fibroblasts: Phenotypic and Functional Switch of Differentiated Cells in Cornea

Cited by 21 publications

References 21 publications

Corneal Stroma Regeneration with Acellular Corneal Stroma Sheets and Keratocytes in a Rabbit Model

Corneal Stroma Regeneration with Acellular Corneal Stroma Sheets and Keratocytes in a Rabbit Model

Chondrogenic Transdifferentiation of Human Dermal Fibroblasts Stimulated with Cartilage-Derived Morphogenetic Protein 1

Reconstructing spinal dura-like tissue using electrospun poly(lactide-co-glycolide) membranes and dermal fibroblasts to seamlessly repair spinal dural defects in goats

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