Tissue Engineering of Blood Vessels: Functional Requirements, Progress, and Future Challenges

Kumar, Vivek; Brewster, Luke P.; Caves, Jeffrey M.; Chaikof, Elliot L.

doi:10.1007/s13239-011-0049-3

Cited by 98 publications

(86 citation statements)

References 163 publications

(156 reference statements)

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“…The compliance of grafts with internal diameters of 1.3 mm and 4 mm was 2.7 ± 0.3%/100 mmHg, and 2.9 ± 0.2%/100 mmHg, respectively, which was similar that of native saphenous vein (0.7 – 2.6%/100 mmHg) (Table 1) [35, 36]. Burst pressures were 830 ± 131 mmHg for 1.3 mm i.d.…”

Section: Resultsmentioning

confidence: 58%

“…Engineered grafts displayed compliance, which approximated that of native vein and adequate suturability. While not as high as native arteries, engineered vessels exhibited burst pressures that were approximately three to four fold greater than maximum physiologic pressures (Table 1) [35]. In this report, the capacity of elastin analogues to undergo a temperature sensitive sol-gel transition provided a means to adhere layers of collagen to each other.…”

Section: Discussionmentioning

confidence: 82%

See 1 more Smart Citation

Acellular vascular grafts generated from collagen and elastin analogs

Kumar¹,

Caves²,

Haller³

et al. 2013

Acta Biomaterialia

Self Cite

145

130

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Tissue engineered vascular grafts require long fabrication times, in part, due to the requirement of cells from a variety of cell sources to produce a robust load bearing, extracellular matrix. Herein, we propose a design strategy for the fabrication of tubular conduits comprised of collagen fiber networks and elastin-like protein polymers to mimic native tissue structure and function. Dense fibrillar collagen networks exhibited an ultimate tensile strength (UTS) of 0.71 ± 0.06 MPa, strain to failure of 37.1 ± 2.2%, and Young’s modulus of 2.09 ± 0.42 MPa, comparing favorably to an UTS and a Young’s modulus for native blood vessels of 1.4 – 11.1 MPa and 1.5 ± 0.3 MPa, respectively. Resilience, a measure of recovered energy during unloading of matrices, demonstrated that 58.9 ± 4.4% of the energy was recovered during loading-unloading cycles. Rapid fabrication of multilayer tubular conduits with maintenance of native collagen ultrastructure was achieved with internal diameters ranging between 1 to 4 mm. Compliance and burst pressures exceeded 2.7 ± 0.3%/100 mmHg and 830 ± 131 mmHg, respectively, with a significant reduction in observed platelet adherence as compared to ePTFE (6.8 ± 0.05 × 105 vs. 62 ± 0.05 × 105 platelets/mm2, p < 0.01). Using a rat aortic interposition model, early in vivo responses were evaluated at 2 weeks via Doppler ultrasound and CT angiography with immunohistochemistry confirming a limited early inflammatory response (n=8). Engineered collagen-elastin composites represent a promising strategy for fabricating synthetic tissues with defined extracellular matrix content, composition, and architecture.

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Section: Resultsmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 82%

Acellular vascular grafts generated from collagen and elastin analogs

Kumar¹,

Caves²,

Haller³

et al. 2013

Acta Biomaterialia

Self Cite

145

130

View full text Add to dashboard Cite

show abstract

“…When considering suture retention strength, multilayered grafts showed a lower force required to pull the suture through the graft compared to pure PCL sutures, which were comparable to the values of native vessels ranging between 0.8 N to 2.5 N reported in other studies. [39] In further experiments, we will consider increasing the thickness of the middle PCL layer, which might lead to even better suture retention strength in multilayered vascular grafts.…”

Section: Discussionmentioning

confidence: 99%

A multilayered electrospun graft as vascular access for hemodialysis

et al. 2017

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Despite medical achievements, the number of patients with end-stage kidney disease keeps steadily raising, thereby entailing a high number of surgical and interventional procedures to establish and maintain arteriovenous vascular access for hemodialysis. Due to vascular disease, aneurysms or infection, the preferred access—an autogenous arteriovenous fistula—is not always available and appropriate. Moreover, when replacing small diameter blood vessels, synthetic vascular grafts possess well-known disadvantages. A continuous multilayered gradient electrospinning was used to produce vascular grafts made of collagen type I nanofibers on luminal and adventitial graft side, and poly-ɛ-caprolactone as medial layer. Therefore, a custom-made electrospinner with robust environmental control was developed. The morphology of electrospun grafts was characterized by scanning electron microscopy and measurement of mechanical properties. Human microvascular endothelial cells were cultured in the graft under static culture conditions and compared to cultures obtained from dynamic continuous flow bioreactors. Immunofluorescent analysis showed that endothelial cells form a continuous luminal layer and functional characteristics were confirmed by uptake of acetylated low-density-lipoprotein. Incorporation of vancomycin and gentamicin to the medial graft layer allowed antimicrobial inhibition without exhibiting an adverse impact on cell viability. Most striking a physiological hemocompatibility was achieved for the multilayered grafts.

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“…[1][2][3][4] The class of materials known as hydrogels, which range from synthetic molecules such as poly(ethylene glycol) to native proteins, such as collagen and fibrin, has been demonstrated to be well-suited for use as 3D scaffolds. [5][6][7][8][9] Collagen-based hydrogels are gaining widespread popularity as scaffolds for tissue engineering due to the abundance of collagen in natural extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine

Vlachos

Rylander³

2014

Tissue Engineering Part B: Reviews

464

353

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Type I collagen hydrogels have been used successfully as three-dimensional substrates for cell culture and have shown promise as scaffolds for engineered tissues and tumors. A critical step in the development of collagen hydrogels as viable tissue mimics is quantitative characterization of hydrogel properties and their correlation with fabrication parameters, which enables hydrogels to be tuned to match specific tissues or fulfill engineering requirements. A significant body of work has been devoted to characterization of collagen I hydrogels; however, due to the breadth of materials and techniques used for characterization, published data are often disjoint and hence their utility to the community is reduced. This review aims to determine the parameter space covered by existing data and identify key gaps in the literature so that future characterization and use of collagen I hydrogels for research can be most efficiently conducted. This review is divided into three sections: (1) relevant fabrication parameters are introduced and several of the most popular methods of controlling and regulating them are described, (2) hydrogel properties most relevant for tissue engineering are presented and discussed along with their characterization techniques, (3) the state of collagen I hydrogel characterization is recapitulated and future directions are proposed. Ultimately, this review can serve as a resource for selection of fabrication parameters and material characterization methodologies in order to increase the usefulness of future collagenhydrogel-based characterization studies and tissue engineering experiments.

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Tissue Engineering of Blood Vessels: Functional Requirements, Progress, and Future Challenges

Cited by 98 publications

References 163 publications

Acellular vascular grafts generated from collagen and elastin analogs

Acellular vascular grafts generated from collagen and elastin analogs

A multilayered electrospun graft as vascular access for hemodialysis

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

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