Tissue engineering of biphasic joint cartilage transplants

Kreklau, B; Sittinger, Michael; Mensing, Markus Benjamin; Voigt, Christian; Berger, Georg; Burmester, Gerd R.; Rahmanzadeh, R.; Groß, Ulrich

doi:10.1016/s0142-9612(99)00061-7

Cited by 107 publications

(54 citation statements)

References 12 publications

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“…To investigate the stability of the integrated layers, interfacial strength at the interfaces between each layer was assessed. Poor interfacial strength leads to delamination of the layers, a problem widely reported in the fabrication of layered scaffold materials for tissue engineering [15,26,27]. Using a custom layer adhesion strength measurement technique, the interfacial layer strength was tested to failure, with delamination of the layers being found to occur within the confines of the mechanically weakest layer (top cartilage layer) rather than at the interface.…”

Section: Discussionmentioning

confidence: 99%

“…The first generation of constructs involved fabrication of two separate scaffolds; one designed to repair the cartilage tissue and the other designed to repair bone tissue. These two scaffolds were subsequently fused together using sutures [25,25] or biological sealants or glues [19,26,27]. Success of such materials has been limited due to poor cellular infiltration through the layers of the structure.…”

Section: Introductionmentioning

confidence: 99%

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A biomimetic multi-layered collagen-based scaffold for osteochondral repair

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

et al. 2014

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“…In analogy cells are cultured on an artificial extracellular matrix in tissue engineering. [7][8][9] In vitro development of functional tissues as used for cartilage repair or for the development of liver modules can only be expected when both components interact in an optimal manner. The goal in tissue engineering is to generate tissue specific features while avoiding atypical protein expression, caused by suboptimal culture conditions and cellular dedifferentiation.…”

Section: Introduction Tmentioning

confidence: 99%

“…It is unknown though how being exposed to the obviously identical environment of an organ-for example, the villous epithelium of small intestine displays a high proliferative capacity while EC and Paneth cell populations within the crypts remain in the interphase. Chondroblasts and osteoblasts proliferate rapidly, 6,8,9 while matrix-producing osteocytes do not divide.…”

Section: Introduction Tmentioning

confidence: 99%

Proliferating Cells versus Differentiated Cells in Tissue Engineering

et al. 2002

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The efficiency of cell or tissue cultures is usually judged by how quickly confluence is reached within a Petri dish or on a scaffold. Growth factors and fetal bovine serum are employed to drive cultured cells from one mitosis to the next as quickly as possible. The tissue specific interphase is extremely short under these conditions, so that the degree of differentiation desired in tissue engineering cannot be achieved. To reach the goal of functional differentiation in vitro mitosis and interphase must be separated experimentally and tailored to the specific requirements of the cell-type used. This could be achieved by a three step concept for tissue-engineering in vitro as we present here. The expansion phase is followed by a phase in which tissue differentiation is initiated. The final phase serves to express and maintain histotypical differentiation of the generated tissue.

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“…Knowing about exact kinetics of the degradation process a stepwise modification of scaffold materials became possible. As a result the risk of tissue repulsion after implantation was decreased by the application of those optimized scaffold materials (Rotter et al 1998(Rotter et al , 1999Kreklau et al 1999;Duda et al 2000Duda et al , 2004Haisch et al 2002;Gille et al 2005). It was finally shown that electrospun polymer scaffolds have proven to be particularly advantageous (Schneider et al 2011(Schneider et al , 2012.…”

Section: Connective Tissue Barriermentioning

confidence: 99%

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

Minuth

Denk

2015

Cytotechnology

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To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEET Ò tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or mounting tissue on it. To support spatial (3D) development a carrier can be placed in various types of perfusion culture containers. In the basic version a constant flow of culture medium provides contained tissue with always fresh nutrition and respiratory gas. For example, epithelia can be transferred to a gradient container, where they are exposed to different fluids at the luminal and basal side. To observe development of tissue under the microscope, in a different type of container a transparent lid and base are integrated. Finally, stem/progenitor cells are incubated in a container filled by an artificial interstitium to support spatial development. In the past years the described system was applied in numerous own and external investigations. To present an actual overview of resulting experimental data, the present paper was written.

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Tissue engineering of biphasic joint cartilage transplants

Cited by 107 publications

References 12 publications

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

Proliferating Cells versus Differentiated Cells in Tissue Engineering

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

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