Tissue engineering interventions for esophageal disorders — Promises and challenges

Kuppan, Purushothaman; Sethuraman, Swaminathan; Krishnan, Uma Maheswari

doi:10.1016/j.biotechadv.2012.03.005

Cited by 64 publications

(53 citation statements)

References 86 publications

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“…[1] This congenital malformation leads to choking, coughing and aspiration pneumonia and if not treated may be lethal. [2] Cancer, Barrett's esophagus, stricture and gastro esophageal reflux disease are acquired diseases severely affects the adult populations. [3] These problems severely affect the normal physiology of an individual which leads the secondary effects such as weight loss, malnutrition and associated metabolic dysfunctions.…”

Section: Introductionmentioning

confidence: 99%

“…[3] These problems severely affect the normal physiology of an individual which leads the secondary effects such as weight loss, malnutrition and associated metabolic dysfunctions. [2] Moreover, the mortality rate is increased globally due to the congenital and other diseases of esophagus. [4] Esophageal disorders are repaired surgically using either autologous grafts or allogenic grafts or xenogenic grafts.…”

Section: Introductionmentioning

confidence: 99%

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Kuppan

Sethuraman

Krishnan

2014

Journal of Biomaterials Science, Polymer Edition

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and PHBV-gelatin were electrospun to obtain defect-free nanofibers by optimizing various process and solution parameters. Tensile strength, Young's modulus, and wettability of PHBV-gelatin nanofibrous scaffold were determined and compared with PHBV nanofibrous scaffold. Our results demonstrate that PHBV-gelatin nanofibers exhibited higher tensile strength and Young's modulus than the PHBV nanofibers. Human esophageal epithelial cells (HEEpiC) were cultured on PHBV and PHBV-gelatin nanofiber showed better cell proliferation in PHBV nanofibrous scaffold than the PHBV-gelatin scaffold after 7 days of culture. HEEpiC cultured on PHBV and PHBV-gelatin nanofibrous scaffold exhibited characteristic epithelial cobblestone morphology after 3 days of culture. Further, the HEEpiC extracellular matrix (ECM) proteins (collagen type IV and laminin) and phenotypic marker proteins (cytokeratin-4 and 14) expressions were significantly higher in PHBV-gelatin nanofibrous scaffold than the PHBV nanofiber scaffold. However, the long-term stability and functional state of the cells on the PHBV scaffold give it an edge over the blend scaffolds. Thus, PHBV-based nanofibrous scaffolds could be explored further as ECM substitutes for the regeneration of esophageal tissue.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Kuppan

Sethuraman

Krishnan

2014

Journal of Biomaterials Science, Polymer Edition

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“…The ideal material for esophageal stent should be: 1. biocompatible; 2. biodegradable, where the degradation speed should be faster than that of the proliferation of esophageal tissue; 3. resist the reflux of gastric juice from the stomach; 4. should not cause infection and inflammation [14]; 5. nontoxic. Chitosan seems fit all categories above.…”

Section: Discussionmentioning

confidence: 99%

Esophageal reconstruction: Combined application of muscle tissue flap and inner chitosan tube stent in rabbits

Liu¹,

Zhang²,

Zhou³

et al. 2016

THC

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Abstract. BACKGROUND:Esophageal reconstruction is the key issue in esophageal surgery. However, currently there is no satisfied technique to repair esophagus after surgery. OBJECTIVE: To combine an inner chitosan stent and a muscle tissue with vascular pedicle to repair the esophageal defect in cervical segment. METHODS: Esophageal defect was repaired using the combination of a muscle tissue flap and a chitosan tube stent in experimental group while only muscle tissue flap was utilized in controls for comparison. One animal in each group was sacrificed at week 3, 6 and 9 after operation respectively to exam the healing status. Barium X-ray was used to evaluate the esophageal status in 12 weeks. RESULTS: Histology showed the inflammatory response in 3 weeks after surgery, the chitosan stent was partially absorbed in 6 weeks, and there was no obvious fibrotic proliferation in experimental group; while the fibrotic proliferation and esophageal stenosis were obvious in controls, the chitosan stent was completely absorbed in 9 weeks, and squamous epidermis cells were observed. Twelve weeks later, the barium swallow went smoothly through the esophagus with noticeable peristalsis in the experimental group; esophageal stenosis without peristalsis was observed in controls. CONCLUSION: The combination of chitosan stent and muscle tissue flap is feasible to reconstruct a partial defect in esophagus.

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“…In order for a tissue-engineered construct to achieve functional properties, it must mimic the architecture of the native tissue [8].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic and synthetic esophageal tissue engineering

et al. 2015

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Tissue engineering interventions for esophageal disorders — Promises and challenges

Cited by 64 publications

References 86 publications

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Esophageal reconstruction: Combined application of muscle tissue flap and inner chitosan tube stent in rabbits

Biomimetic and synthetic esophageal tissue engineering

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