Tissue engineering in urethral reconstruction—an update

Mangera, Altaf; Chapple, Christopher R.

doi:10.1038/aja.2012.91

Cited by 46 publications

(38 citation statements)

References 30 publications

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“…This technique requires harvesting autologous cells, culturing cells, and seeding them on the biomaterial before urethroplasty. Due to its complex procedure, complicated technology and high recurrence rate, tissue-engineered urethra is not widely utilized in clinical practice [9][10][11] .…”

mentioning

confidence: 99%

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Xu¹,

Shen

Liu

et al. 2017

Urol Int

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Objective: To investigate the efficacy of using everted saphenous vein graft for urethral reconstruction. Materials and Methods: Thirty-five adult male rabbits were divided into 7 groups randomly: experimental group A, B, C, D, E, stricture control group and normal control group (n = 5). In experimental groups and the stricture control group, a urethral mucosa defect (1.5 × 0.8 cm) was created in each rabbit. In experimental groups, a 2-cm long saphenous vein graft was harvested and incised longitudinally and urethral reconstruction was carried out using the everted saphenous vein patch. Rabbits in experimental group A-E were killed respectively at 1 week, 2 weeks, 1 month, 3 months, and 1 year postoperatively, and the specimens were obtained for histo-pathological examination. Retrograde urethrography was performed to evaluate urethral patency before sacrifice in group D and the stricture control group. Results: In the histo-pathological study, the vein grafts were visible within first week. The vein graft was completely covered by epithelium 1 month postoperatively. Retrograde urethrograms showed the urethral caliber of experimental rabbits were similar to those of normal. While the stricture control group showed a narrow urethral lumen and urothelium defect. Conclusions: For urethral reconstruction, everted saphenous vein graft can be an ideal substitute material because of its longer survival time and rapid epithelization capacity.

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mentioning

confidence: 99%

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Xu¹,

Shen

Liu

et al. 2017

Urol Int

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“…Chung et al have delivered solid proof to support this theory .He used foreskin graft epithelia in co culture in vitro with buccal connective tissue, and directed it to develop into structures resembling oral mucosal epithelium [63]. There is also research proving, that grafts engineered with the use of cells extracted from oral mucosa can function in reconstruction of other epithelial tissues in vivo [64], [65]. However, this method is not ideal due to the limited source material, different patterns of regional epithelial keratinization and surgical procedures involved in its extraction.…”

Section: Oral Mucosal Epithelial Cellsmentioning

confidence: 94%

Does migrative and proliferative capability of epithelial cells reflect cellular developmental competence?

Jankowski

Dyszkiewicz-Konwińska

Budna

et al. 2018

Medical Journal of Cell Biology

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Mammalian epithelial and epithelial-like cells are significantly involved in various processes associated with tissue development, differentiation and oncogenesis. Because of that, high number of research is focused on identifying cells that express stem-like or progenitor characteristics. Identifying such cells and recognizing their specific markers, would open new clinical opportunities in transplantology and oncology. There are several epithelia characterized by their ability to rapidly proliferate and/or differentiate. Due to their function or location they are subject to cyclic changes involving processes of apoptosis and regeneration. Literature presenting well-structured studies of these types of epithelia was analyzed in order to compare various results and establish if epithelial cells' migrative and proliferative ability indicates their stemness potential. Endometrial, ovarian, oviductal and oral mucosal epithelia were analyzed with most of the publications delivering relatively unified results. The ability to rapidly proliferate/ differentiate usually indicated the presence of some kind of stem/stem-like/progenitor cells. Most of the papers focused on pinpointing the exact location of these kind of cells, or analyzing specific markers that would be used for their future identification. There have also been substantial proportion of research that focused on discovering growth factors or intercellular signals that induced proliferation/differentiation in analyzed epithelia. Most of the research provided valuable insights into the modes of function and characteristics of the analyzed tissue, outlining the importance of such study for the possible clinical application of in vitro derived cell cultures.

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“…Any of the buccal, lingual or labial sites may be used. The various complications of harvesting these grafts include immediate intraoperative hemorrhage, pain and swelling, damage to the salivary ducts and limited oral opening due to scarring [19]. Postoperative infection of both the donor and graft sites and limited oral opening are potentially disastrous side effects.…”

Section: Bioengineeringmentioning

confidence: 98%

“…The more conservative methods such as urethral dilatation and endoscopic incision give way to anastomotic and substitution urethroplasty techniques [19]. The sheer complexity of the urethra in terms of the transition of types of epithelia from proximal to distal and the musculature makes the development of substitutes exceptionally challenging.…”

Section: Bioengineeringmentioning

confidence: 99%

Use of Hormones, Tissue Factors and Bioengineering in the Management of Hypospadias

Mitra

Sarin

2017

Indian J Pediatr

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Hypospadiology is a rapidly evolving field. Progress in the understanding of how hormonal therapy affects the growth of the phallus has allowed surgeons to optimize the tissues for surgery. But conflicting data from a number of studies and a lack of consensus on drugs, their dosing, mode of delivery and timing of use means that the creation of protocols is unlikely to happen in the near future. Nonetheless, there is a hope and the standardization of scientific reporting will make it easier to compare data at the global level. There are reports of the increasing incidence of hypospadias and the etiology is thought to be multifactorial. Although complex interactions between genetic polymorphisms and the environment make it difficult to identify the exact factors responsible for hypospadias, the advent of massively parallel gene sequencing, large scale epigenetic screens and CRISPR technology will definitely ease the process. The knowledge of culprit genes will not only broaden our understanding of embryology and growth but will also enable us to predict and/or modify tissue healing. Advances in tissue engineering are also expected to provide a plethora of biomaterials for urethral reconstruction. The development of this field is directly linked with the elucidation of the processes of proliferation and vascularization coupled with the cataloguing of the growth factors involved. One can safely conclude that the exciting new advances in the field will have far reaching consequences on patient care and counselling.

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Tissue engineering in urethral reconstruction—an update

Cited by 46 publications

References 30 publications

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Does migrative and proliferative capability of epithelial cells reflect cellular developmental competence?

Use of Hormones, Tissue Factors and Bioengineering in the Management of Hypospadias

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