Tissue Engineering in Plastic Surgery

Sterodimas, Aris; Faria, Jose de; Correa, Wanda Elizabeth; Pitanguy, Ivo

doi:10.1097/sap.0b013e3181788ec9

Cited by 65 publications

(19 citation statements)

References 61 publications

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“…72-76 Because primary cells from vocal fold tissue are not readily available and would not be a clinically viable selection, human bone-marrow derived MSCs were employed in cell studies; these cells are appropriate for future use in vocal fold cell-gel therapies. The ability of the cross-linked RGD-containing RLP-based hydrogels to support the attachment of hMSCs was investigated via seeding cells on the surface of 20wt% hydrogels with a 50% RLP and 50% RLP-RGD composition.…”

Section: Resultsmentioning

confidence: 99%

Resilin-like polypeptide hydrogels engineered for versatile biological function

et al. 2013

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Natural resilin, the rubber-like protein that exists in specialized compartments of most arthropods, possesses excellent mechanical properties such as low stiffness, high resilience and effective energy storage. Recombinantly-engineered resilin-like polypeptides (RLPs) that possess the favorable attributes of native resilin would be attractive candidates for the modular design of biomaterials for engineering mechanically active tissues. Based on our previous success in creating a novel RLP-based hydrogel and demonstrating useful mechanical and cell-adhesive properties, we have produced a suite of new RLP-based constructs, each equipped with 12 repeats of the putative resilin consensus sequence and a single, distinct biologically active domain. This approach allows independent control over the concentrations of cell-binding, MMP-sensitive, and polysaccharide-sequestration domains in hydrogels comprising mixtures of the various RLPs. The high purity, molecular weight and correct compositions of each new polypeptide have been confirmed via high performance liquid chromatography (HPLC), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and amino acid analysis. These RLP-based polypeptides exhibit largely random-coil conformation, both in solution and in the cross-linked hydrogels, as indicated by circular dichroic and infrared spectroscopic analyses. Hydrogels of various compositions, with a range of elastic moduli (1kPa to 25kPa) can be produced from these polypeptides, and the activity of the cell-binding and matrix metalloproteinase (MMP) sensitive domains was confirmed. Tris(hydroxymethyl phosphine) cross-linked RLP hydrogels were able to maintain their mechanical integrity as well as the viability of encapsulated primary human mesenchymal stem cells (MSCs). These results validate the promising properties of these RLP-based elastomeric biomaterials.

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Section: Resultsmentioning

confidence: 99%

Resilin-like polypeptide hydrogels engineered for versatile biological function

et al. 2013

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“…The field combines cell transplantation, biomaterial science, bioengineering techniques and biochemical expertise to deliver both biologic and artificial components for permanent repair, designed specifically for the patient [1]. The ideal properties of a biomimetic scaffold include; good and rapid adherence; ease of handling and application; rapid bio-integration allowing neovascularisation; and, it must provide an environment that supports cell attachment, growth, proliferation and formation of extracellular construct (ECM).…”

Section: Introductionmentioning

confidence: 99%

Effects of photochemical riboflavin-mediated crosslinks on the physical properties of collagen constructs and fibrils

Rich

Odlyha

Cheema

et al. 2013

J Mater Sci: Mater Med

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The use of collagen scaffold in tissue engineering is on the rise, as modifications to mechanical properties are becoming more effective in strengthening constructs whilst preserving the natural biocompatibility. The combined technique of plastic compression and cross-linking is known to increase the mechanical strength of the collagen construct. Here, a modified protocol for engineering these collagen constructs is used to bring together a plastic compression method, combined with controlled photochemical crosslinking using riboflavin as a photoinitiator. In order to ascertain the effects of the photochemical crosslinking approach and the impact of the crosslinks created upon the properties of the engineered collagen constructs, the constructs were characterized both at the macroscale and at the fibrillar level. The resulting constructs were found to have a 2.5 fold increase in their Young’s modulus, reaching a value of 650 ± 73 kPa when compared to non-crosslinked control collagen constructs. This value is not yet comparable to that of native tendon, but it proves that combining a crosslinking methodology to collagen tissue engineering may offer a new approach to create stronger, biomimetic constructs. A notable outcome of crosslinking collagen with riboflavin is the collagen’s greater affinity for water; it was demonstrated that riboflavin crosslinked collagen retains water for a longer period of time compared to non-cross-linked control samples. The affinity of the cross-linked collagen to water also resulted in an increase of individual collagen fibrils’ cross-sectional area as function of the crosslinking. These changes in water affinity and fibril morphology induced by the process of crosslinking could indicate that the crosslinked chains created during the photochemical crosslinking process may act as intermolecular hydrophilic nanosprings. These intermolecular nanosprings would be responsible for a change in the fibril morphology to accommodate variable volume of water within the fibril.

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“…f 7) or still through integration: (14) By inverting it, the solutions class is obtained: (15) where sn is Jacobi's elliptic function of module k [34]. For 0  k , the relation (15) can be approximated by: (18) we can "control" and "direct" the potentiality of informational energy in the Onicescu sense (for details, see [35]).…”

Section: Theoretical Mathematical Modelmentioning

confidence: 99%

“…It has also been found that platelets density in the carrier system is overwhelming important in stimulating the bone repair process. Although most studies indicate a 5-to 10-fold higher platelet density at the PRP level than serum levels, the ratio in which platelet concentrate to the carrier system should be used is unknown [13,14].…”

mentioning

confidence: 99%

Accelerating the Bone Healing Process by the Intervention of the Platelet Growth Factors Impregnated in Collagen. An experimental and theoretical mathematical model

Susanu¹,

Popescu²,

Caba³

et al. 2019

Mat.Plast.

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A bone defect with standard dimensions and localization is produced. Inside this defect a polymer (collagen sponge) impregnated with growing factors, i. e. bone morphogenetic protein - BMP, is introduced. For the witness lot, the same defect is produced, in which the same polymer is introduced, but without any growing factors. It is found that the growing factor not only remains at the initial site, but also has effect on the bone regeneration process. A mathematical model is constructed using a field theory of multifractal type based on the spontaneous symmetry breaking mechanism to exploit such dynamics in biostructures. This mechanism contains all the informational �ingredients�: the multivalent logic based multifractal bit, the algorithm based networks through spatial cnoidal modes of oscilation, etc. In such context, the evolution of all biostructures, through a mechanism that mimics a 3D biological printer, can become operational.

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Tissue Engineering in Plastic Surgery

Cited by 65 publications

References 61 publications

Resilin-like polypeptide hydrogels engineered for versatile biological function

Resilin-like polypeptide hydrogels engineered for versatile biological function

Effects of photochemical riboflavin-mediated crosslinks on the physical properties of collagen constructs and fibrils

Accelerating the Bone Healing Process by the Intervention of the Platelet Growth Factors Impregnated in Collagen. An experimental and theoretical mathematical model

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