Tissue engineering in orthopaedic sports medicine: current concepts

Costa, João B.; Pereira, Hélder; Espregueira-Mendes, João; Khang, Gilson; Oliveira, Joaquím M.; Reis, Rui L.

doi:10.1136/jisakos-2016-000080

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…Tissue-engineering (TE) approaches, which use combinations of cells, scaffolds, and biomolecules to regenerate diseased or damaged tissues, are promising methods that can yield satisfactory clinical outcomes [ 2 ]. TE approaches have the added advantage of not requiring another part of the patient's body for grafting to regenerate damaged tissues.…”

Section: Introductionmentioning

confidence: 99%

Osteochondral tissue engineering: Perspectives for clinical application and preclinical development

Lee

Tan

et al. 2021

Journal of Orthopaedic Translation

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The treatment of osteochondral defects (OCD) remains challenging. Among currently available surgical treatments for OCDs, scaffold-based treatments are promising to regenerate the osteochondral unit. However, there is still no consensus regarding the clinical effectiveness of these scaffold-based therapies for OCDs. Previous reviews have described the gradient physiological characteristics of osteochondral tissue and gradient scaffold design for OCD, tissue engineering strategies, biomaterials, and fabrication technologies. However, the discussion on bridging the gap between the clinical need and preclinical research is still limited, on which we focus in the present review, providing an insight into what is currently lacking in tissue engineering methods that failed to yield satisfactory outcomes, and what is needed to further improve these techniques. Currently available surgical treatments for OCDs are firstly summarized, followed by a comprehensive review on experimental animal studies in recent 5 years on osteochondral tissue engineering. The review will then conclude with what is currently lacking in these animal studies and the recommendations that would help enlighten the community in developing more clinically relevant implants. The translational potential of this article This review is attempting to summarize the lessons from clinical and preclinical failures, providing an insight into what is currently lacking in TE methods that failed to yield satisfactory outcomes, and what is needed to further improve these implants.

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Section: Introductionmentioning

confidence: 99%

Osteochondral tissue engineering: Perspectives for clinical application and preclinical development

Lee

Tan

et al. 2021

Journal of Orthopaedic Translation

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“…Several strategies have already been proposed, but only few of them have been shown to be effective [4]. Tissue engineering (TE) approaches have been applied in the orthopedic field, namely for meniscus regeneration showing very promising preclinical results and high potential for clinical applications in a near future [5]. Associated to advances in engineering of scaffolds, patient specificity is becoming an important requisite in many TE approaches and orthopedic applications, including the treatment of meniscus lesions [6].…”

Section: Introductionmentioning

confidence: 99%

Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

et al. 2019

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The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered scaffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of scaffolds produced using different SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced scaffolds presented homogeneous porosity in both layers and no differences were observed when comparing both scaffolds. A decrease in terms of mechanical performance of the scaffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical scaffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quantification analysis. Subcutaneous implantation in mice revealed weak inflammation and scaffold's integrity. The hierarchical indirect printed SF scaffolds can be promising candidate for meniscus TE scaffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patient-specific approach.

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“…A cirurgia para reconstrução óssea utiliza como padrão ouro o enxerto ósseo autólogo, porém devido a sua quantidade limitada e morbidade do sitio doador aumentaram as buscas por novos métodos que sejam eficazes no reparo ósseo (BOULER et al, 2017). A partir dessa necessidade, a engenharia de tecidos tem desenvolvido biomateriais que possam estimular a rápida proliferação celular óssea tornando-os assim uma alternativa interessante para a medicina regenerativa (MIJIRITSKY et al, 2017;RATNAYAKE;MUCALO;DIAS, 2017;COSTA et al, 2017 (ORYAN et al, 2014;JANICKI;SCHMIDMAIER, 2011).…”

Section: Discussionunclassified

Estudo comparativo de membrana serosa porcina e de elastina no reparo de defeitos na calvária de ratos

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Tissue engineering in orthopaedic sports medicine: current concepts

Cited by 8 publications

References 52 publications

Osteochondral tissue engineering: Perspectives for clinical application and preclinical development

Osteochondral tissue engineering: Perspectives for clinical application and preclinical development

Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

Estudo comparativo de membrana serosa porcina e de elastina no reparo de defeitos na calvária de ratos

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