Tissue Engineering for Human Urethral Reconstruction: Systematic Review of Recent Literature

Kemp, Vincent de; Graaf, Petra de; Fledderus, Joost O.; Bosch, J.L.H. Ruud; Kort, Laetitia M.O. de

doi:10.1371/journal.pone.0118653

Cited by 83 publications

(87 citation statements)

References 60 publications

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“…An exhaustive review by de Kemp et al [21], analysed in detail the evolution of tissue engineering for urethral reconstruction. While in-vitro experimentation may provide heartening results, clinical application is a different ball game altogether.…”

Section: Bioengineeringmentioning

confidence: 99%

Use of Hormones, Tissue Factors and Bioengineering in the Management of Hypospadias

Mitra

Sarin

2017

Indian J Pediatr

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Hypospadiology is a rapidly evolving field. Progress in the understanding of how hormonal therapy affects the growth of the phallus has allowed surgeons to optimize the tissues for surgery. But conflicting data from a number of studies and a lack of consensus on drugs, their dosing, mode of delivery and timing of use means that the creation of protocols is unlikely to happen in the near future. Nonetheless, there is a hope and the standardization of scientific reporting will make it easier to compare data at the global level. There are reports of the increasing incidence of hypospadias and the etiology is thought to be multifactorial. Although complex interactions between genetic polymorphisms and the environment make it difficult to identify the exact factors responsible for hypospadias, the advent of massively parallel gene sequencing, large scale epigenetic screens and CRISPR technology will definitely ease the process. The knowledge of culprit genes will not only broaden our understanding of embryology and growth but will also enable us to predict and/or modify tissue healing. Advances in tissue engineering are also expected to provide a plethora of biomaterials for urethral reconstruction. The development of this field is directly linked with the elucidation of the processes of proliferation and vascularization coupled with the cataloguing of the growth factors involved. One can safely conclude that the exciting new advances in the field will have far reaching consequences on patient care and counselling.

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Section: Bioengineeringmentioning

confidence: 99%

Use of Hormones, Tissue Factors and Bioengineering in the Management of Hypospadias

Mitra

Sarin

2017

Indian J Pediatr

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“…Tissue engineering technology using cell‐seeded or unseeded matrices to reconstruct the urethra has been used in various studies with mixed results . The Buccal epithelium Expanded and Encapsulated in Scaffold‐Hybrid Approach to Urethral Stricture (BEES‐HAUS) is a tissue engineering‐based cell therapy procedure that is completely endoscopic and is a much less morbid procedure.…”

Section: Introductionmentioning

confidence: 99%

Buccal epithelium Expanded and Encapsulated in Scaffold‐Hybrid Approach to Urethral Stricture (BEES‐HAUS) procedure: A novel cell therapy‐based pilot study

2018

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Objectives To describe the feasibility of a novel cell‐based endoscopic technique using buccal epithelium, expanded and encapsulated in a thermoreversible gelation polymer scaffold for the treatment of urethral stricture. Methods Six male patients with bulbar urethral stricture ranging from 2.0 to 3.5 cm in length were included in this pilot study. Autologous buccal epithelial cells from a small buccal mucosal biopsy were isolated, cultured and encapsulated in thermoreversible gelation polymer scaffold, and were implanted at the stricture site after a wide endoscopic urethrotomy. Results All the patients voided well, with a mean peak flow rate of 24 mL/s. Urethroscopy carried out at 6 months showed healthy mucosa at the urethrotomy site. However, two of the six patients had recurrence at 18 and 24 months, respectively. Conclusions This endoscopic‐based Buccal epithelium Expanded and Encapsulated in Scaffold‐Hybrid Approach to Urethral Stricture (BEES‐HAUS) technique is a promising alternative for the open substitution buccal graft urethroplasty. It is possible to achieve the benefits of open substitution buccal urethroplasty with this endoscopic technique.

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“…Some authors affirm that the urothelium and the urethral epithelium have same embryologic origin, but the genes expressed by the cells present in the urothelium are not the same with those expressed by the cells present in the urethral epithelium (de Graaf et al, 2017). In humans, for the urethral reconstruction is used the urothelium or the oral mucosal cells, but the urethral grafts are not fully successful (de Kemp et al, 2015). It is very important to know the structure of urethral epithelium in young animals in reconstruction of the urethral wall after different pathological situations, such as urethral stricture or congenital disorders.…”

Section: Introductionmentioning

confidence: 99%

The Structure of Urethral Epithelium in Merinos Lambs

Rus¹,

Matosz²,

Ruxanda³

2018

BUASVMCN-VM

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The aim of this study was to investigate by histological techniques the structure of urethral epithelium in lambs. In this study, we harvested several fragments (prostatic, membranous and cavernous) from urethra from 5 merino's lambs of 3 months old. The first anatomical segment, the prostatic urethra, is lined by a urinary epithelium. The intermediary layer of this epithelium is formed of 5-6 rows of oval cells. The second segment of urethra has the same type of epithelium but the intermediary layer is formed of 6-7 rows of oval cells. In the last anatomical segment, the penile urethra, the epithelium is the same, but the intermediary layer has 3-4 rows of oval cells. In lambs, the urethra is lined by urinary epithelium. The urethral epithelium does not have the same thickness in all segments. The thinner epithelium it is in the cavernous urethra, the ticker is the membranous urethra.

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Tissue Engineering for Human Urethral Reconstruction: Systematic Review of Recent Literature

Cited by 83 publications

References 60 publications

Use of Hormones, Tissue Factors and Bioengineering in the Management of Hypospadias

Use of Hormones, Tissue Factors and Bioengineering in the Management of Hypospadias

Buccal epithelium Expanded and Encapsulated in Scaffold‐Hybrid Approach to Urethral Stricture (BEES‐HAUS) procedure: A novel cell therapy‐based pilot study

The Structure of Urethral Epithelium in Merinos Lambs

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