Tissue engineering for full-thickness burns: A dermal substitute from bench to bedside

Kellouche, Sabrina; Martin, Christophe; Korb, Gregory; Rezzonico, Roger; Bouard, Delphine; Benbunan, M; Dubertret, Louis; Soler, Christophe; Legrand, Chantal; Dosquet, Christine

doi:10.1016/j.bbrc.2007.08.155

Cited by 26 publications

(15 citation statements)

References 15 publications

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“…Epidermal stem cells have an important role in wound repair and tissue engineering of replacement skin (Kellouche et al, 2007;Xie et al, 2007). To date, however, the limited capacity for the isolation or purification of significant numbers of epidermal stem cells has hampered their wide clinical application.…”

Section: Introductionmentioning

confidence: 99%

Wnt/β‐catenin signaling is critical for dedifferentiation of aged epidermal cells in vivo and in vitro

et al. 2011

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SummaryAged epidermal cells have the capacity to dedifferentiate into stem cell-like cells. However, the signals that regulate the dedifferentiation of aged epidermal cells remain unclear. Here, we provide evidence that Wnt/b-catenin is critical for aged epidermal cell dedifferentiation in vivo and in vitro. Some aged epidermal cells in human ultrathin epidermal sheets lacking basal stem cells transplanted onto wounds dedifferentiated into stem cell-like cells that were positive for CK19 and b1 integrin but negative for CK10. In addition, Wnt/b-catenin pathway was activated during this process. There was increased expression of Wnt-1, Wnt-4, Wnt-7a, b-catenin, cyclin D1, and c-myc. Secreted frizzled-related protein 1, a Wnt/b-catenin pathway inhibitor, blocked dedifferentiation in vivo. Then, the activator, a highly specific glycogen synthase kinase (GSK)-3b inhibitor, of Wnt/b-catenin pathway was added to the culture medium of aged epidermal cells. Surprisingly, we found that the activator induced higher expression of CK19, b1 integrin, Oct4, and Nanog proteins. The induced aged epidermal cells exhibited high colony-forming efficiency, long-term proliferative potential and could regenerate a skin equivalent (as do epidermal stem cells). These results suggested that activation of Wnt/b-catenin pathway induced the dedifferentiation of aged epidermal cells, which suggest a new approach to generate epidermal stem cell-like cells.

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Section: Introductionmentioning

confidence: 99%

Wnt/β‐catenin signaling is critical for dedifferentiation of aged epidermal cells in vivo and in vitro

et al. 2011

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“…Some of them are still in the process of in vitro investigation; however, some have entered stage II-III clinical trials and could possibly be on the market shortly available for patients and health practitioners. Novel potential skin substitute biomaterials and scaffolds include human hair keratin-collagen sponge , hyaluronan coupled with fibronectin functional domains (Ghosh et al 2006), poly(lactic-co-glycolic acid)/chitosan hybrid nanofibrous membrane (Duan et al 2007), biodegradable polyurethane microfibres (Rockwood et al 2007), polycaprolactone (PCL) collagen nanofibrous membrane (Venugopal et al 2006), silk fibroin and alginate (Roh et al 2006), polyvinyl alcohol/chitosan/fibroin blended sponge (Yeo et al 2000), Tegaderm-nanofibre construct (Chong et al 2007), bacterial cellulose (Helenius et al 2006), ICX-SKN skin graft replacement (Boyd et al 2007), porcine collagen paste (Shevchenko et al 2008), bovine collagen cross-linked with microbial transglutaminase (Garcia et al 2008), collagen-glycosaminoglycanchitosan dermal matrix seeded with fibroblasts (Kellouche et al 2007), composite nano-titanium oxide-chitosan artificial skin (Peng et al 2008), keratinocytes and fibroblasts grown on Collatamp, deacetylated chitin or plant cellulose transfer membranes (Johnen et al 2008) and many others (table 4). Some of these experimental biomaterials, like PermaDerm, have produced promising clinical results (Boyce et al 1999 and have a potential to be licensed and marketed for clinical use.…”

Section: Potential Biomaterials For Skin Substitutionmentioning

confidence: 99%

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Shevchenko

James

2009

J. R. Soc. Interface.

613

469

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Situations where normal autografts cannot be used to replace damaged skin often lead to a greater risk of mortality, prolonged hospital stay and increased expenditure for the National Health Service. There is a substantial need for tissue-engineered skin bioconstructs and research is active in this field. Significant progress has been made over the years in the development and clinical use of bioengineered components of the various skin layers. Off-the-shelf availability of such constructs, or production of sufficient quantities of biological materials to aid rapid wound closure, are often the only means to help patients with major skin loss. The aim of this review is to describe those materials already commercially available for clinical use as well as to give a short insight to those under development. It seeks to provide skin scientists/tissue engineers with the information required to not only develop in vitro models of skin, but to move closer to achieving the ultimate goal of an off-the-shelf, complete full-thickness skin replacement.

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“…Autologous or allogeneic PLT concentrate lysates (PCLs), often referred to as PLT‐rich plasma, are used in dentistry; implantology; and orthopedic, plastic, and reconstructive surgery to stimulate the regeneration of hard tissues . They promote the wound healing process of soft tissues and are used for instance to treat recalcitrant leg ulcers and burns . Local injection of PLT lysates has given preliminary encouraging clinical results for reducing pain and improving knee osteoarthritis .…”

mentioning

confidence: 99%

Virally inactivated human platelet concentrate lysate induces regulatory T cells and immunosuppressive effect in a murine asthma model

Lee

et al. 2013

Transfusion

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Tissue engineering for full-thickness burns: A dermal substitute from bench to bedside

Cited by 26 publications

References 15 publications

Wnt/β‐catenin signaling is critical for dedifferentiation of aged epidermal cells in vivo and in vitro

Wnt/β‐catenin signaling is critical for dedifferentiation of aged epidermal cells in vivo and in vitro

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Virally inactivated human platelet concentrate lysate induces regulatory T cells and immunosuppressive effect in a murine asthma model

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