Tissue engineering approaches for the in vitro production of spermatids to treat male infertility: A review

Bhaskar, Rakesh; Gupta, Mukesh Kumar; Han, Sung Soon

doi:10.1016/j.eurpolymj.2022.111318

Cited by 7 publications

(5 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conventional treatments for of male infertility and subfertility are still not effective related to problems associated to spermatogenesis disorders ( 170 ). Several testicular cell culture systems have been studied to mimetize the testicular microenvironment and restore fertility.…”

Section: Discussionmentioning

confidence: 99%

“…Ramzgouyan et al (2015) (241) were able to differentiate germ celllike cells on fibrin hydrogel, demonstrating the biocompatibility of the differentiated cells to the hydrogel. Although little studied, fibrin scaffolds have high porosity and biocompatibility, characteristics that can be used for the development of seminiferous tubule niches in testicular tissue (170).…”

Section: Natural Biomaterials For Testicular Bioengineeringmentioning

confidence: 99%

See 1 more Smart Citation

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

View full text Add to dashboard Cite

Traditional therapeutic interventions aim to restore male fertile potential or preserve sperm viability in severe cases, such as semen cryopreservation, testicular tissue, germ cell transplantation and testicular graft. However, these techniques demonstrate several methodological, clinical, and biological limitations, that impact in their results. In this scenario, reproductive medicine has sought biotechnological alternatives applied for infertility treatment, or to improve gamete preservation and thus increase reproductive rates in vitro and in vivo. One of the main approaches employed is the biomimetic testicular tissue reconstruction, which uses tissue-engineering principles and methodologies. This strategy pursues to mimic the testicular microenvironment, simulating physiological conditions. Such approach allows male gametes maintenance in culture or produce viable grafts that can be transplanted and restore reproductive functions. In this context, the application of several biomaterials have been proposed to be used in artificial biological systems. From synthetic polymers to decellularized matrixes, each biomaterial has advantages and disadvantages regarding its application in cell culture and tissue reconstruction. Therefore, the present review aims to list the progress that has been made and the continued challenges facing testicular regenerative medicine and the preservation of male reproductive capacity, based on the development of tissue bioengineering approaches for testicular tissue microenvironment reconstruction.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Natural Biomaterials For Testicular Bioengineeringmentioning

confidence: 99%

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Since the 1990s, further co-culture has been evaluated by combining isolated germline cells and/or spermatogonial stem cell (SSC) with somatic cell lines. More recently, the seeding of either isolated germline cells or co-culture methods on biocompatible scaffolds has been explored to achieve in vitro spermatogenesis ( Bhaskar et al, 2022 ), including further focus on the importance of not only the scaffold structure but also the culture methods and media conditions comprising the cellular microenvironment. All methods described above applied testis-derived cells to a wide array of culture conditions and have successfully yielded haploid cells.…”

Section: History Of Testicular Tissue Engineeringmentioning

confidence: 99%

De novo testicular tissue generation from non-testicular cell lines, biologic and synthetic scaffolds: Current findings and future translational applications

Hosseini

DeBenedetto

Jiang

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

In recent decades, reproductive science has revolutionized the options for biological parenthood for the 20–50% of infertility cases affected by male factors. However, current solutions exclude those who are infertile due to absent testicular tissue. This includes anorchic 46, XY individuals due to trauma or congenital factors and transgender men with a 46, XX genotype. There is a clinical need for methods to restore testicular function independent of pre-existing testicular tissue. This mini-review analyzes studies that have applied non-testicular cell lines to generate germline and non-germline testicular parenchymal components. While only 46, XY cell lines have been evaluated in this context to date, the potential for future application of cell lines from 46, XX individuals is also included. Additionally, the role of varied culture methods, media supplementation, and biologic and synthetic scaffolds to further support testicular parenchyma generation are critiqued. De novo testicular tissue generation in this manner will require a focus on both cellular and environmental aspects of tissue engineering. Put together, these studies highlight the future potential for expanded clinical, reproductive, and endocrine management options for individuals who are currently excluded from aspects of biologic reproduction most consistent with their gender identity and reproductive preferences.

show abstract

“…Subsequently, these preserved SSCs or tissue fragments can be engrafted on a three-dimensional (3D) substrate by TT engineering. These 3D-culture systems provide a suitable microenvironment for cell attachment and specific growth factors for testicular regeneration [7]. The emergence of advanced bioengineered systems has offered new hope for maintaining male fertility through the development of functional male germ cells.…”

Section: Introductionmentioning

confidence: 99%

In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration

Bashiri,

Hosseini,

Salem

et al. 2024

Clin Exp Reprod Med

View full text Add to dashboard Cite

Male infertility can be caused by genetic anomalies, endocrine disorders, inflammation, and exposure to toxic chemicals or gonadotoxic treatments. Therefore, several recent studies have concentrated on the preservation and restoration of fertility to enhance the quality of life for affected individuals. It is currently recommended to biobank the tissue extracted from testicular biopsies to provide a later source of spermatogonial stem cells (SSCs). Another successful approach has been the in vitro production of haploid male germ cells. The capacity of SSCs to transform into sperm, as in testicular tissue transplantation, SSC therapy, and in vitro or ex vivo spermatogenesis, makes them ideal candidates for in vivo fertility restoration. The transplantation of SSCs or testicular tissue to regenerate spermatogenesis and create embryos has been achieved in nonhuman mammal species. Although the outcomes of human trials have yet to be released, this method may soon be approved for clinical use in humans. Furthermore, regenerative medicine techniques that develop tissue or cells on organic or synthetic scaffolds enriched with bioactive molecules have also gained traction. All of these methods are now in different stages of experimentation and clinical trials. However, thanks to rigorous studies on the safety and effectiveness of SSC-based reproductive treatments, some of these techniques may be clinically available in upcoming decades.

show abstract

Tissue engineering approaches for the in vitro production of spermatids to treat male infertility: A review

Cited by 7 publications

References 152 publications

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

De novo testicular tissue generation from non-testicular cell lines, biologic and synthetic scaffolds: Current findings and future translational applications

In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration

Contact Info

Product

Resources

About