Tissue Engineering and Regenerative Medicine 2015: A Year in Review

Wobma, Holly; Vunjak‐Novakovic, Gordana

doi:10.1089/ten.teb.2015.0535

Cited by 67 publications

(53 citation statements)

References 103 publications

(106 reference statements)

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“…More generally, the successful miniaturization of the nanoelectronic scaffolds, which allows matching the size and mechanical properties to conventional passive tissues scaffolds, and the incorporation of large numbers of addressable nanoelectronic devices, which allows 3D mapping, can enable facile integration in a range of engineered tissues for drug screening models through regenerative medicine broadly defined 32 . For example, utilizing softer nanoelectronic and auxiliary scaffolds we could extend the application to engineered 3D neuronal tissues 19 .…”

Section: Simultaneous Mapping and Manipulation Of Cardiac Activitymentioning

confidence: 99%

Three-dimensional mapping and regulation of action potential propagation in nanoelectronics-innervated tissues

et al. 2016

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Real-time mapping and manipulation of electrophysiology in three-dimensional (3D) tissues could impact broadly fundamental scientific and clinical studies, yet realization lacks effective methods. Here we introduce tissue-scaffold-mimicking 3D nanoelectronic arrays consisting of 64 addressable devices with subcellular dimensions and sub-millisecond time-resolution. Real-time extracellular action potential (AP) recordings reveal quantitative maps of AP propagation in 3D cardiac tissues, enable in situ tracing of the evolving topology of 3D conducting pathways in developing cardiac tissues, and probe the dynamics of AP conduction characteristics in a transient arrhythmia disease model and subsequent tissue self-adaptation. We further demonstrate simultaneous multi-site stimulation and mapping to manipulate actively the frequency and direction of AP propagation. These results establish new methodologies for 3D spatiotemporal tissue recording and control, and demonstrate the potential to impact regenerative medicine, pharmacology and electronic therapeutics.

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Section: Simultaneous Mapping and Manipulation Of Cardiac Activitymentioning

confidence: 99%

Three-dimensional mapping and regulation of action potential propagation in nanoelectronics-innervated tissues

et al. 2016

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“…In nature, ECM mechanics are one of key characteristics to adjust and support the differentiation and function of the corresponding cells. 30-32 It has been found that the immature brain of rat and pig exhibited roughly twice as stiff as adult brain, demonstrating larger force requirement for brain development. 33,34 Moreover, many studies have demonstrated that nerve-related cells could sense and respond to mechanical cues from the culture substrate.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional hyaluronic acid hydrogel-based models for in vitro human iPSC-derived NPC culture and differentiation

Duan

et al. 2017

J. Mater. Chem. B

100

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Human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) are considered as a promising cell source for transplantation and have been used for organoid fabrication to recapitulate central nervous system (CNS) diseases in vitro. The establishment of three-dimensional (3D) in vitro model with hiPSC-NPCs and control of their differentiation is significantly critical for understanding biological processes and CNS disease and regeneration. Here we implemented 3D methacrylated hyaluronic acid (Me-HA) hydrogels with encapsulation of hiPSC-NPCs as in vitro culture models and further investigated the role of the hydrogel rigidity on the cell behavior of hiPSC-NPCs. We first encapsulated single dispersive hiPSC-NPCs within both soft and stiff Me-HA hydrogel and found that hiPSC-NPCs gradually self-assembled and aggregated to form 3D spheroids. Then, the hiPSC-NPCs were laden into Me-HA hydrogels in the form of spheroids to evaluate their spontaneous differentiation in response to hydrogel rigidity. The soft Me-HA hydrogel-encapsulated hiPSC-NPCs displayed robust neurite outgrowth and showed high levels of spontaneous neural differentiation. We further encapsulated Down Syndrome (DS) patient-specific hiPSC-derived NPCs (DS-NPCs) spheroids within our hydrogels. DS-NPCs remained excellent cell viability in both soft and stiff Me-HA hydrogels. Similarly, soft hydrogels promoted neural differentiation of DS-NPCs by significantly upregulating neural maturation markers. This study demonstrates that soft matrix promotes neural differentiation of hiPSC-NPCs and HA-based hydrogels with hiPSC-NPCs or DS-NPCs are effective 3D models for CNS disease study.

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“…1 The final goal is to create artificial three-dimensional (3D) scaffolds that sufficiently mimic the natural biological environments, thereby biological cells in the artificial environments can function as well as they would in the real tissue. For designing artificial biological scaffolds, structural geometry and morphology should be tuned for high functionality and extracellular matrix (ECM) mimicry at various length scales to recapitulate the tissue complexity and engineer the desired organ.…”

Section: Introductionmentioning

confidence: 99%

Laser-assisted biofabrication in tissue engineering and regenerative medicine

et al. 2016

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Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies.

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Tissue Engineering and Regenerative Medicine 2015: A Year in Review

Cited by 67 publications

References 103 publications

Three-dimensional mapping and regulation of action potential propagation in nanoelectronics-innervated tissues

Three-dimensional mapping and regulation of action potential propagation in nanoelectronics-innervated tissues

Three-dimensional hyaluronic acid hydrogel-based models for in vitro human iPSC-derived NPC culture and differentiation

Laser-assisted biofabrication in tissue engineering and regenerative medicine

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