Tissue engineering and auricular reconstruction: a review

Sterodimas, Aris; Faria, Jose de; Correa, Wanda Elizabeth; Pitanguy, Ivo

doi:10.1016/j.bjps.2008.11.046

Cited by 59 publications

(48 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The tissue consists of mainly chondrocyctes and an ECM containing collagens type I and II and aggregan (Sterodimas et al 2009). A large amount of research has focussed on tissue engineering cartilage by the encapsulation of chondrocyctes in a variety of hydrogels including fibrin, collagen, chitosan and alginate.…”

Section: Cartilagementioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

View full text Add to dashboard Cite

To cite this version:Nicola C. Hunt, Liam M. Grover. Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnology Letters, Springer Verlag, 2010, 32 (6), pp.733-742. <10.1007/s10529-010-0221-0>. Abstract There has been a consistent increase in the mean life expectancy of the population of the developed world over the past century. Healthy life expectancy, however, has not increased concurrently. As a result we are living a larger proportion of our lives in poor health and there is a growing demand for the replacement of diseased and damaged tissues. While traditionally tissue grafts have functioned well for this purpose, the demand for tissue grafts now exceeds the supply. For this reason, research in regenerative medicine is rapidly expanding to cope with this new demand. There is now a trend towards supplying cells with a material in order to expedite the tissue healing process. Hydrogel encapsulation provides cells with a three dimensional environment similar to that experienced in vivo and therefore may allow the maintenance of normal cellular function in order to produce tissues similar to those found in the body. In this review we discuss biopolymeric gels that have been used for the encapsulation of mammalian cells for tissue engineering applications as well as a brief overview of cell encapsulation for therapeutic protein production. This review focuses on agarose, alginate, collagen, fibrin, hyaluronic acid and gelatin since they are widely used for cell encapsulation. The literature on the regeneration of cartilage, bone, ligament, tendon, skin, blood vessels and neural tissues using these materials has been summarised.

show abstract

Section: Cartilagementioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

View full text Add to dashboard Cite

show abstract

“…137 Tissue engineering strategy has been considered as an alternative means to reconstruct auricular defects. 138 Creation of an auricle built with patient's own cells is attractive as this approach eliminates the concerns associated with immunological rejection or foreign body reaction. Engineering of cartilage tissue de novo for total auricular reconstruction does require an extensive cell expansion process and lengthy wait time while the new cartilage tissue forms.…”

Section: Tissue Engineering Applicationsmentioning

confidence: 99%

“…More importantly, the current limitations of tissue-engineered cartilage tissue constructs for clinical application are primarily due to the challenges in maintaining tissue shape resulting from poor mechanical strength and long-term stability in vivo. 138 To overcome these limitations, we previously have introduced an approach that utilizes alloplastic implants coated with autologous chondrocytes. 139 The chondrocytes coated on the surface of implant material are able to form a cartilage tissue layer covering the entire ear implant.…”

Section: Tissue Engineering Applicationsmentioning

confidence: 99%

Recent Applications of Polymeric Biomaterials and Stem Cells in Tissue Engineering and Regenerative Medicine

Lee

Yoo

Atala³

2014

Polymer Korea

View full text Add to dashboard Cite

Tissue engineering and regenerative medicine strategies could offer new hope for patients with serious tissue injuries or end-stage organ failure. Scientists are now applying the principles of cell transplantation, material science, and engineering to create biological substitutes that can restore and maintain normal function in diseased or injured tissues/ organs. Specifically, creation of engineered tissue construct requires a polymeric biomaterial scaffold that serves as a cell carrier, which would provide structural support until native tissue forms in vivo. Even though the requirements for scaffolds may be different depending on the target applications, a general function of scaffolds that need to be fulfilled is biodegradability, biological and mechanical properties, and temporal structural integrity. The scaffold's internal architecture should also enhance the permeability of nutrients and neovascularization. In addition, the stem cell field is advancing, and new discoveries in tissue engineering and regenerative medicine will lead to new therapeutic strategies. Although use of stem cells is still in the research phase, some therapies arising from tissue engineering endeavors that make use of autologous adult cells have already entered the clinic. This review discusses these tissue engineering and regenerative medicine strategies for various tissues and organs.

show abstract

“…However, several critical problems are associated with this approach, including low chondrogenic potential owing to poor matrix production and a tendency toward vascularization and hypertrophic transition [10][11][12][13][14][15][16]. Using hyaline cartilage, such as costal cartilage, for elastic cartilage reconstruction (the most common approach) is also problematic because it forms tissue with less elastic fiber, different stiffness, and low resistance to deformation [10].…”

Section: Introductionmentioning

confidence: 99%

Xiphoid Process-Derived Chondrocytes: A Novel Cell Source for Elastic Cartilage Regeneration

Nam

Cho

et al. 2014

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Reconstruction of elastic cartilage requires a source of chondrocytes that display a reliable differentiation tendency. Predetermined tissue progenitor cells are ideal candidates for meeting this need; however, it is difficult to obtain donor elastic cartilage tissue because most elastic cartilage serves important functions or forms external structures, making these tissues indispensable. We found vestigial cartilage tissue in xiphoid processes and characterized it as hyaline cartilage in the proximal region and elastic cartilage in the distal region. Xiphoid process-derived chondrocytes (XCs) showed superb in vitro expansion ability based on colony-forming unit fibroblast assays, cell yield, and cumulative cell growth. On induction of differentiation into mesenchymal lineages, XCs showed a strong tendency toward chondrogenic differentiation. An examination of the tissue-specific regeneration capacity of XCs in a subcutaneous-transplantation model and autologous chondrocyte implantation model confirmed reliable regeneration of elastic cartilage regardless of the implantation environment. On the basis of these observations, we conclude that xiphoid process cartilage, the only elastic cartilage tissue source that can be obtained without destroying external shape or function, is a source of elastic chondrocytes that show superb in vitro expansion and reliable differentiation capacity. These findings indicate that XCs could be a valuable cell source for reconstruction of elastic cartilage. STEM CELLS

show abstract

Tissue engineering and auricular reconstruction: a review

Cited by 59 publications

References 31 publications

Cell encapsulation using biopolymer gels for regenerative medicine

Cell encapsulation using biopolymer gels for regenerative medicine

Recent Applications of Polymeric Biomaterials and Stem Cells in Tissue Engineering and Regenerative Medicine

Xiphoid Process-Derived Chondrocytes: A Novel Cell Source for Elastic Cartilage Regeneration

Contact Info

Product

Resources

About