Tissue-engineered solution containing cells and biomaterials—an in vitro study: A perspective as a novel therapeutic application

Abstract

Some biomaterial scaffolds can positively interfere with tissue regeneration and are being developed to successfully repair the tissue function. The possibility of using epithelial cells combined with biomaterials appears to be a new option as therapeutic application. This combination emerges as a possibility for patients with Mayer–Rokitansky–Kuster–Hauser syndrome which requires vaginal repair and can be performed with tissue-engineered solution containing cells and biomaterials. It is expected that tissue-engineered solution containing cells and biomaterials would promote tissue repair in a more efficient, modern, and safe way. This study tested the efficiency of tissue-engineered solution containing human malignant melanoma cell line (HMV-II) and different biomaterials, including Cellprene®, Membracel®, and poly lactic-co-glycolic acid/epoxidized polyisoprene. The cells adhered better on poly lactic-co-glycolic acid/epoxidized polyisoprene, and it was found that tissue-engineered solution may also contain mesenchymal stem cells cultivated on poly lactic-co-glycolic acid/epoxidized polyisoprene. Histological, immunofluorescence, and scanning electron microscopy analyses were performed. These initial in vitro results suggest that tissue-engineered solution containing cells and poly lactic-co-glycolic acid/epoxidized polyisoprene is a potential for tissue reconstruction.

Latex and natural rubber: recent advances for biomedical applications

“…Cellprene ® was developed and patented by the Federal University of Rio Grande do Sul (Brazil) in 2013 for application in tissue engineering [65,90] . According to the literature, Cellprene ® has been used in recent studies demonstrating that this biomaterial can act in the maintenance of epithelial cells [65,91,92]…”

Electrospun natural rubber latex biocomposite for scaffolds in tissue engineering

“…Human adipose-derived mesenchymal stem cells (AD-MSCs) were seeded in six wells plate at a density of 2 × 10 5 cells/well by 48 h culture time containing 14-mm-diameter PLGA/PIepox and prior hydration followed by Henckes et al protocol. 19 The AD-MSCs were cultured in Dulbecco's Modified Eagle Medium (Gibco, USA), supplemented with 20% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Gibco). Cell culture was incubated at 37°C in a humidified atmosphere containing 5% CO 2 .…”

Scaffold strategies combined with mesenchymal stem cells in vaginal construction: a review

“…Our group has recently developed a PLGA/PIepox scaffold and the in vitro model MSCs have shown biological features in the proliferation ability in poly (lactic-co-glycolic acid)/ epoxidized poly (isoprene) (PLGA/PIepox) scaffold (Henckes et al, 2019 ; Guerra et al, 2018 ). As a result, many efforts have been focusing in the application of technologies involving an in vivo model approach to further extend the use to clinical practice as well as to restore or repair reproductive organs and other organs with similar tissue structures therapies.…”

“…These promising results might bring innovative solutions. In this vein, considering the advances in experiments (Henckes et al, 2019 ; Guerra et al, 2020 ; Guerra et al, 2018 ), the option of tissue engineering with the combination of mesenchymal stem cells and scaffolds for patients who require additional tissues and need immediate and multiple reconstructive surgeries has increased considerably. Certainly, the use of seeded cells-therapy combined with scaffolds for vaginal reconstruction and others abnormalities is promising and requires further investigation.…”

“…Schopf’s study demonstrated the occurrence of an inflammatory reaction in the adjacent tissue to PLGA/PI polymeric implanted fragment (Schopf et al, 2018 ). In a recent progress, novel studies were conducted to improve and overcome limitations in the process of PLGA/PI (Cellprene®) fabrication as well as its chemical composition where modifications have been applied and new applications were tested, resulting in a new scaffold epoxidized called PLGA/PIepox (Guerra et al, 2018 ; Henckes et al, 2019 ).…”