Tissue-Engineered Skin Substitute Enhances Wound Healing after Radiation Therapy

Chowdhury, Shiplu Roy; Ismail, Fuad; Saim, Aminuddin Bin; Idrus, Ruszymah Bt Hj

doi:10.1097/01.asw.0000480556.78111.e4

Cited by 20 publications

(12 citation statements)

References 37 publications

(20 reference statements)

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“…Integrity of the epidermis layer is evaluated by the presence of tight junctions known as desmosomes between one keratinocyte and another, while the adhesion of the epidermis to the dermis at the basal membrane is evaluated through the presence of hemidesmosomes 44 . Since the dermis layer is primarily made up of collagen, the collagen fibrils, which are present in cross section or longitudinal section, depict the integrity of the dermis 45 . The TEM results revealed that the regenerated skin’s integrity of mice in the HA and HC groups is better compared to the NT group, suggesting that the healing process is still in progress in the NT group.…”

Section: Resultsmentioning

confidence: 99%

Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

Loh

Mohamad

Fauzi

et al. 2018

Sci Rep

140

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Bacterial cellulose (BC)/acrylic acid (AA) hydrogel has successfully been investigated as a wound dressing for partial-thickness burn wound. It is also a promising biomaterial cell carrier because it bears some resemblance to the natural soft tissue. This study assessed its ability to deliver human epidermal keratinocytes (EK) and dermal fibroblasts (DF) for the treatment of full-thickness skin lesions. In vitro studies demonstrated that BC/AA hydrogel had excellent cell attachment, maintained cell viability with limited migration, and allowed cell transfer. In vivo wound closure, histological, immunohistochemistry, and transmission electron microscopy evaluation revealed that hydrogel alone (HA) and hydrogel with cells (HC) accelerated wound healing compared to the untreated controls. Gross appearance and Masson’s trichrome staining indicated that HC was better than HA. This study suggests the potential application of BC/AA hydrogel with dual functions, as a cell carrier and wound dressing, to promote full-thickness wound healing.

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Section: Resultsmentioning

confidence: 99%

Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

Loh

Mohamad

Fauzi

et al. 2018

Sci Rep

140

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“…As such, this could be a good model to reduce the compound wound contraction effect in animal model. Even though a lot of research has been used the humanized mice model with splint to estimate the re-epithelialization in human skin wound healing, but this model is unable to provide sufficient information of complex interplay of wound healing process, which includes surrounding wound environment, integration of native tissue with the provisional 3D bio-scaffold and the migration of neighbour cells into the scaffold (Busra et al, 2016;Chantre et al, 2018). In addition, the granulation, dermal regeneration, and remodelling cannot be quantified efficiently due to the starting point of re-epithelialization on the surface area is unclear (Jimi, De Francesco, Ferraro, Riccio, & Hara, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Human skin sample was obtained as redundant tissue from six consented patients undergoing abdominoplasty, and processed as described previously (Busra, Chowdhury, Ismail, Saim, & Idrus, 2016;Xian et al, 2015). Briefly, 3 cm 2 skin samples were cleaned, minced, and digested using 0.6% collagenase type I (Worthington, USA) at 37°C for 4-6 hr followed by treatment with trypsin-EDTA (TE; Lonza, USA) for 10 min.…”

Section: Isolation and Culture Of Skin Cellsmentioning

confidence: 99%

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Rapid treatment of full‐thickness skin loss using ovine tendon collagen typeIscaffold with skin cells

Fauzi

Rajab

Tabata

et al. 2019

J Tissue Eng Regen Med

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The full‐thickness skin wound is a common skin complication affecting millions of people worldwide. Delayed treatment of this condition causes the loss of skin function and integrity that could lead to the development of chronic wounds or even death. This study was aimed to develop a rapid wound treatment modality using ovine tendon collagen type I (OTC‐I) bio‐scaffold with or without noncultured skin cells. Genipin (GNP) and carbodiimide (EDC) were used to cross‐link OTC‐I scaffold to improve the mechanical strength of the bio‐scaffold. The physicochemical, biomechanical, biodegradation, biocompatibility, and immunogenicity properties of OTC‐I scaffolds were investigated. The efficacy of this treatment approach was evaluated in an in vivo skin wound model. The results demonstrated that GNP cross‐linked OTC‐I scaffold (OTC‐I_GNP) had better physicochemical and mechanical properties compared with EDC cross‐linked OTC‐I scaffold (OTC‐I_EDC) and noncross‐link OTC‐I scaffold (OTC‐I_NC). OTC‐I_GNP and OTC‐I_NC demonstrated no toxic effect on cells as it promoted higher cell attachment and proliferation of both primary human epidermal keratinocytes and human dermal fibroblasts compared with OTC‐I_EDC. Both OTC‐I_GNP and OTC‐I_NC exhibited spontaneous formation of bilayer structure in vitro. Immunogenic evaluation of OTC‐I scaffolds, in vitro and in vivo, revealed no sign of immune response. Finally, implantation of OTC‐I_NC and OTC‐I_GNP scaffolds with noncultured skin cells demonstrated enhanced healing with superior skin maturity and microstructure features, resembling native skin in contrast to other treatment (without noncultured skin cells) and control group. The findings of this study, therefore, suggested that both OTC‐I scaffolds with noncultured skin cells could be promising for the rapid treatment of full‐thickness skin wound.

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“…Tissue scaffolds realized using natural and synthetical materials are able to provide a physical barrier, by covering the wound, and thus to stimulate the re-epithelialization process. Among natural polymers collagen, fibrin, chitosan, gelatin, and elastin have been developed as biological skin substitutes for wound healing [ 107 – 111 ]. Several collagen-based dressings in the form of a gel, sheet, lattice, or sponge are commercially available and are successful used as temporary covering for ulcers and burns [ 112 ].…”

Section: Applications Of Nanostructured Materials In Tementioning

confidence: 99%

Fabrication and Applications of Micro/Nanostructured Devices for Tissue Engineering

et al. 2016

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Nanotechnology allows the realization of new materials and devices with basic structural unit in the range of 1–100 nm and characterized by gaining control at the atomic, molecular, and supramolecular level. Reducing the dimensions of a material into the nanoscale range usually results in the change of its physiochemical properties such as reactivity, crystallinity, and solubility. This review treats the convergence of last research news at the interface of nanostructured biomaterials and tissue engineering for emerging biomedical technologies such as scaffolding and tissue regeneration. The present review is organized into three main sections. The introduction concerns an overview of the increasing utility of nanostructured materials in the field of tissue engineering. It elucidates how nanotechnology, by working in the submicron length scale, assures the realization of a biocompatible interface that is able to reproduce the physiological cell–matrix interaction. The second, more technical section, concerns the design and fabrication of biocompatible surface characterized by micro- and submicroscale features, using microfabrication, nanolithography, and miscellaneous nanolithographic techniques. In the last part, we review the ongoing tissue engineering application of nanostructured materials and scaffolds in different fields such as neurology, cardiology, orthopedics, and skin tissue regeneration.

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Tissue-Engineered Skin Substitute Enhances Wound Healing after Radiation Therapy

Abstract: These results indicate that BTESS is the preferred treatment for irradiated wound ulcers.

Cited by 20 publications

References 37 publications

Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

Rapid treatment of full‐thickness skin loss using ovine tendon collagen typeIscaffold with skin cells

Fabrication and Applications of Micro/Nanostructured Devices for Tissue Engineering

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