Tissue‐Engineered Skin Containing Mesenchymal Stem Cells Improves Burn Wounds

Liu, Peng; Deng, Zhihong; Han, Shuang; Liu, Tao; Wen, Ning; Lu, Wei; Geng, Xianhui; Huang, Sha; Jin, Yan

doi:10.1111/j.1525-1594.2008.00654.x

Cited by 136 publications

(87 citation statements)

References 23 publications

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“…However, in the case of deep injuries such as extensive burns and subsequent persistent infection due to loss of skin barrier function, a limit is reached where the epithelium self-healing cannot occur. 6,7 This effect leads to a significant loss of tissue function and might also act as a facilitating factor for other problems such as infection.…”

Section: Introductionmentioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing damaged tissues. An intact epithelium plays an indispensable role in the functionality of several organs such as the trachea, esophagus, and cornea. Furthermore, the integrity of the epithelial barrier and its degree of differentiation would define the level of success in tissue engineering of other organs such as the bladder and the skin. In this review, we focus on the challenges and requirements associated with engineering of epithelial layers in different tissues. Functional epithelial layers can be achieved by methods such as cell sheets, cell homing, and in situ epithelialization. However, for organs composed of several tissues, other important factors such as (1) in vivo epithelial cell migration, (2) multicell-type differentiation within the epithelium, and (3) epithelial cell interactions with the underlying mesenchymal cells should also be considered. Recent successful clinical trials in tissue engineering of the trachea have highlighted the importance of a functional epithelium for long-term success and survival of tissue replacements. Hence, using the trachea as a model tissue in clinical use, we describe the optimal structure of an artificial epithelium as well as challenges of obtaining a fully functional epithelium in macroscale. One of the possible remedies to address such challenges is the use of bottom-up fabrication methods to obtain a functional epithelium. Modular approaches for the generation of functional epithelial layers are reviewed and other emerging applications of microscale epithelial tissue models for studying epithelial/mesenchymal interactions in healthy and diseased (e.g., cancer) tissues are described. These models can elucidate the epithelial/mesenchymal tissue interactions at the microscale and provide the necessary tools for the next generation of multicellular engineered tissues and organ-on-a-chip systems.

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Section: Introductionmentioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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“…[2][3][4][5] MSCs seeded on synthetic or naturally occurring matrices have been used to produce precisely shaped constructs to replace diseased or damaged tissues. 4,[6][7][8] Although these findings are exciting, several factors limit this technique from becoming routine in clinical practice. One of the biggest obstacles to the use of tissue-engineered constructs in reconstruction of tissue defects is the lengthy culture and manufacturing periods required by conventional techniques.…”

Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Growth Factor Inhibits Bone Morphogenetic Protein 2 Expression in Rat Mesenchymal Stem Cells

Schönmeyr

Soares

Avraham

et al. 2010

Tissue Engineering Part A

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“…Bone marrow stromal cells and subcutaneous preadipocytes can promote epidermal regeneration in a skin reconstruction model [Aoki et al, 2004]. Tissue-engineered skin containing bone marrow mesenchymal stem cells can promote the healing of burn wounds [Liu et al, 2008]. When keratinocytes were seeded directly onto the fat cell layer, they proliferated extensively and generated an epidermis [Sugihara et al, 2001].…”

Section: Introductionmentioning

confidence: 99%

Mixture of Fibroblasts and Adipose Tissue-Derived Stem Cells Can Improve Epidermal Morphogenesis of Tissue-Engineered Skin

Zhang

et al. 2011

Cells Tissues Organs

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Many studies demonstrate that the type of adjacent mesenchymal cells can affect epidermal morphogenesis of bilayered tissue-engineered skin. However, whether a mixture of different mesenchymal cell types can improve epidermal morphogenesis of bioengineered skin remains unknown. In this study, keratinocytes, dermal fibroblasts and adipose tissue-derived stem cells (ADSCs) were isolated and purified from human skin and subcutaneous fat. Conditioned medium generated from a mixture of dermal fibroblasts and ADSCs at the ratio of 1:1 was superior to that from fibroblasts or ADSCs alone in promoting keratinocyte proliferation, as indicated by MTT assay. Furthermore, ELISA results showed that the cytokine levels of human hepatocyte growth factor and keratinocyte growth factor (also known as FGF7) in the mixed fibroblasts/ADSC group were higher than those in the ADSC or dermal fibroblasts group. To examine the potential roles of mixed fibroblasts and ADSCs on epidermal morphogenesis, a three-dimensional tissue engineered skin system was applied. Histological analyses demonstrated that keratinocytes proliferated extensively over the mixture of fibroblasts and ADSCs, and formed a thick epidermal layer with well-differentiated structures. Keratin 10 (epidermal differentiation marker) was expressed in the suprabasal layer of bilayered tissue-engineered skin in the mixed fibroblasts and ADSCs group. Desmosomes and hemidesmosomes were detected in the newly formed epidermis by transmission electron microscopy analysis. Together, these findings revealed for the first time that a mixture of fibroblasts and ADSCs in bilayered tissue-engineered skin can improve epidermal morphogenesis.

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Tissue‐Engineered Skin Containing Mesenchymal Stem Cells Improves Burn Wounds

Cited by 136 publications

References 23 publications

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vascular Endothelial Growth Factor Inhibits Bone Morphogenetic Protein 2 Expression in Rat Mesenchymal Stem Cells

Mixture of Fibroblasts and Adipose Tissue-Derived Stem Cells Can Improve Epidermal Morphogenesis of Tissue-Engineered Skin

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