Tissue engineered scaffolds for corneal endothelial regeneration: a material's perspective

Delaey, Jasper; Vos, Lobke De; Koppen, Carina; Dubruel, Peter; Vlierberghe, Sandra Van; Bogerd, Bert Van den

doi:10.1039/d1bm02023d

Cited by 11 publications

(2 citation statements)

References 105 publications

(130 reference statements)

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“…The human cornea has a thickness of ≈500 µm. [ 40 ] During the preoperative period, the corneal graft needs to be saturated with swelling, and it is critical to control the wet state thickness of the composite membrane. By studying the swelling ratio of the composite membrane in deionized water, the thickness change is determined.…”

Section: Resultsmentioning

confidence: 99%

Hydrophilic, Porous, Fiber‐Reinforced Collagen‐Based Membrane for Corneal Repair

Li,

Liu,

et al. 2024

Macromolecular Bioscience

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Collagen membrane with outstanding biocompatibility exhibits immense potential in the field of corneal repair and reconstruction, but the poor mechanical properties limit its clinical application. Polycaprolactone (PCL) is a biodegradable polymer that has been widely explored for application in corneal reconstruction due to its excellent mechanical properties, biocompatibility, easy processability, and flexibility. In this study, a PCL/collagen composite membrane with reinforced mechanical properties has been developed. The membrane has a strong composite structure with collagen by utilizing a porous and hydrophilic PCL scaffold, maintaining its integrity even after immersion. The suture retention and mechanical tests demonstrate that compared with the pure collagen membrane, the prepared membrane has a greater tensile strength and twice the modulus of elasticity. Further, the suture retention strength is improved by almost two times. In addition, the membrane remains fully intact on the implant bed in an in vitro corneal defect model. Moreover, the membrane could be tightly sutured to a rabbit corneal defect, progressively achieved epithelialization, and remained unchanged during observation. Overall, the PCL/collagen composite membrane is a promising candidate as a suturable corneal restoration material in clinical keratoplasty.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Hydrophilic, Porous, Fiber‐Reinforced Collagen‐Based Membrane for Corneal Repair

Li,

Liu,

et al. 2024

Macromolecular Bioscience

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“…9 The qualified acellular porcine cornea can withstand the pulling of sutures during transplantation, support the curved structure of the cornea after transplantation, reshape the corneal, induce stromal cell infiltration and epithelial cell migration on the corneal surface, accelerate fusion with the host cornea, quickly repair the tissue, and restore corneal function. 10 Ensuring its sterility without compromising its structural integrity and affecting its clinical performance is the key aspect for its wide clinical application. A good standardized irradiation sterilization method has been applied to acellular xenotransplantation, most of which have a good prognosis without graft rejection, transparent loss, or neovascularization.…”

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confidence: 99%

Structure and properties of the acellular porcine cornea irradiated with ⁶⁰Co‐γ and electron beam and its histocompatibility

Li,

Sun,

Zhao

et al. 2023

J Biomedical Materials Res

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Acellular porcine cornea (APC) has been used in corneal transplantation and treatment of the corneal diseases. Sterilization is a key step before the application of graft, and irradiation is one of the most commonly used methods. In this paper, APC was prepared by the physical freeze‐thawing combined with biological enzymes, and the effects of the electron beam (E‐beam) and cobalt 60 (60Co‐γ) at the dose of 15 kGy on the physicochemical properties, structure, immunogenicity, and biocompatibility of the APC were investigated. After decellularization, the residual DNA was 20.86 ± 1.02 ng/mg, and the α‐Gal clearance rate was more than 99%. Irradiation, especially the 60Co‐γ, reduced the cornea's transmittance, elastic modulus, enzymatic hydrolysis rate, swelling ratio, and cross‐linking degree. Meanwhile, the diameter and spacing of the collagen fibers increased. In the rat subcutaneous implantation, many inflammatory cells appeared in the unirradiated APC, while the irradiated had good histocompatibility, but the degradation was faster. The lamellar keratoplasty in rabbits indicated that compared to the E‐beam, the 60Co‐γ damaged the chemical bond of collagen to a larger extent, reduced the content of GAGs, and prolonged the complete epithelization of the grafts. The corneal edema was more serious within 1 month after the surgery. After 2 months, the thickness of the APC with the two irradiation methods tended to be stable, but that in the 60Co‐γ group became thinner. The pathological results showed that the collagen structure was looser and the pores were larger, indicating the 60Co‐γ had a more extensive effect on the APC than the E‐beam at 15 kGy.

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Multilayer amnion-PCL nanofibrous membrane loaded with celecoxib exerts a therapeutic effect against tendon adhesion by improving the inflammatory microenvironment

Liu,

Zhang,

Zhao

et al. 2023

Heliyon

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Tissue engineered scaffolds for corneal endothelial regeneration: a material's perspective

Abstract: An overview of scaffolds for corneal endothelial tissue engineering, their properties and materials starting from the Descemet's membrane and evaluating the scaffolds from a materials scientist perspective.

Cited by 11 publications

References 105 publications

Hydrophilic, Porous, Fiber‐Reinforced Collagen‐Based Membrane for Corneal Repair

Hydrophilic, Porous, Fiber‐Reinforced Collagen‐Based Membrane for Corneal Repair

Structure and properties of the acellular porcine cornea irradiated with ⁶⁰Co‐γ and electron beam and its histocompatibility

Multilayer amnion-PCL nanofibrous membrane loaded with celecoxib exerts a therapeutic effect against tendon adhesion by improving the inflammatory microenvironment

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Tissue engineered scaffolds for corneal endothelial regeneration: a material's perspective

Abstract: An overview of scaffolds for corneal endothelial tissue engineering, their properties and materials starting from the Descemet's membrane and evaluating the scaffolds from a materials scientist perspective.

Cited by 11 publications

References 105 publications

Hydrophilic, Porous, Fiber‐Reinforced Collagen‐Based Membrane for Corneal Repair

Hydrophilic, Porous, Fiber‐Reinforced Collagen‐Based Membrane for Corneal Repair

Structure and properties of the acellular porcine cornea irradiated with 60Co‐γ and electron beam and its histocompatibility

Multilayer amnion-PCL nanofibrous membrane loaded with celecoxib exerts a therapeutic effect against tendon adhesion by improving the inflammatory microenvironment

Contact Info

Product

Resources

About

Structure and properties of the acellular porcine cornea irradiated with ⁶⁰Co‐γ and electron beam and its histocompatibility