Tissue-engineered mucosa graft for reconstruction of the intraoral lining after freeing of the tongue: A clinical and immunohistologic study

Lauer, Günter; Schimming, Ronald

doi:10.1053/joms.2001.20489

Cited by 74 publications

(60 citation statements)

References 15 publications

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“…This indicates that keratin 6 expression and proliferation are constructs have been described in a clinical setting. Two of these consist only of cultured mucosa keratinocytes not dependent on each other but are characteristics of the same tissue, and is in agreement with our previous grown on an acellular matrix (20,22,23). While these initial clinical results look promising [average time for findings (16).…”

Section: Discussionsupporting

confidence: 91%

Comparison of Autologous Full-Thickness Gingiva and Skin Substitutes for Wound Healing

et al. 2008

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Ideally tissue-engineered products should maintain the characteristics of the original tissue. For example, skin represents orthokeratinized epithelium and oral gingiva represents parakeratinized epithelium. The aim of this study was to develop an autologous full-thickness gingiva substitute suitable for clinical applications and to compare it with our autologous full-thickness skin substitute that is routinely used for healing chronic wounds. Autologous full-thickness skin and gingiva substitutes were constructed under identical culture conditions from 3-mm punch biopsies isolated from the upper leg or gingiva tissue, respectively. Both consisted of reconstructed epithelia on acellular dermis repopulated with fibroblasts. To compare the characteristics of the original and reconstructed tissue, differential morphological observations and expression of differentiation markers (keratins 6, 10, and 17 and stratum corneum precursors involucrin, loricrin, and SKALP) were determined. Skin and gingiva substitutes were transplanted onto therapy-resistant leg ulcers or tooth extraction sites in order to determine their effects on wound healing. The tissue-engineered constructs maintained many of the differential histological and immunohistochemical characteristics of the original tissues from which they were derived. The skin substitute was orthokeratinized, and the gingiva substitute was parakeratinized. Transplantation of skin (n = 19) and gingiva substitutes (n = 3) resulted in accelerated wound healing with no adverse effects. As identical culture systems were used to generate both the skin and gingiva substitutes, the differences observed in tissue (immuno)histology can be attributed to intrinsic properties of the tissues rather than to environmental factors (e.g., air or saliva). This study emphasizes the importance of closely matching donor sites with the area to be transplanted. Our results represent a large step forward in the area of clinical applications in oral tissue engineering, which have until now greatly lagged behind skin tissue engineering.

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Section: Discussionsupporting

confidence: 91%

Comparison of Autologous Full-Thickness Gingiva and Skin Substitutes for Wound Healing

et al. 2008

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“…81 Clinical replacement of oral mucosa with tissue engineered grafts resulted in fully differentiated, functional oral mucosa in a 6-month interval. 82 For other organs, such as the liver, there are also tissue engineered constructs that are at the clinical trials. 83 Epithelium in clinical trachea tissue engineering.…”

Section: Epithelium In Clinical Full Organ/multicellular Tissue-enginmentioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing damaged tissues. An intact epithelium plays an indispensable role in the functionality of several organs such as the trachea, esophagus, and cornea. Furthermore, the integrity of the epithelial barrier and its degree of differentiation would define the level of success in tissue engineering of other organs such as the bladder and the skin. In this review, we focus on the challenges and requirements associated with engineering of epithelial layers in different tissues. Functional epithelial layers can be achieved by methods such as cell sheets, cell homing, and in situ epithelialization. However, for organs composed of several tissues, other important factors such as (1) in vivo epithelial cell migration, (2) multicell-type differentiation within the epithelium, and (3) epithelial cell interactions with the underlying mesenchymal cells should also be considered. Recent successful clinical trials in tissue engineering of the trachea have highlighted the importance of a functional epithelium for long-term success and survival of tissue replacements. Hence, using the trachea as a model tissue in clinical use, we describe the optimal structure of an artificial epithelium as well as challenges of obtaining a fully functional epithelium in macroscale. One of the possible remedies to address such challenges is the use of bottom-up fabrication methods to obtain a functional epithelium. Modular approaches for the generation of functional epithelial layers are reviewed and other emerging applications of microscale epithelial tissue models for studying epithelial/mesenchymal interactions in healthy and diseased (e.g., cancer) tissues are described. These models can elucidate the epithelial/mesenchymal tissue interactions at the microscale and provide the necessary tools for the next generation of multicellular engineered tissues and organ-on-a-chip systems.

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“…Besides distinct "master genes" which control the progression of osteogenic differentiation, it is believed that this process is controlled also by a cascade of molecular events involving a combination of genetic programming and gene regulation by various molecules such as alkaline phosphatase, osteopontin, bone sialoprotein, and osteonectin (Wang, 2003). The tissue engineering approach for bone regeneration and repair is a new challenge in oral and maxillofacial surgery (Bianco and Robey, 2001;Yamada et al, 2004;Lauer and Schimming, 2001;Zietek et al, 2008). Bone regeneration could be supported by a scaffold that has to be biocompatible, biodegradable, and able to support cell growth and differentiation (Huang et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Functional assay, expression of growth factors and proteins modulating bone-arrangement in human osteoblasts seeded on an anorganic bovine bone biomaterial

Trubiani

Fulle²,

Traini³

et al. 2010

eCM

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The basic aspects of bone tissue engineering include chemical composition and geometry of the scaffold design, because it is very important to improve not only cell attachment and growth but especially osteodifferentiation, bone tissue formation, and vascularization. Geistlich BioOss ® (GBO) is a xenograft consisting of deproteinized, sterilized bovine bone, chemically and physically identical to the mineral phase of human bone.In this study, we investigated the growth behaviour and the ability to form focal adhesions on the substrate, using vinculin, a cytoskeletal protein, as a marker. Moreover, the expression of bone specific proteins and growth factors such as type I collagen, osteopontin, bone sialoprotein, bone morphogenetic protein-2 (BMP-2), BMP-7 and de novo synthesis of osteocalcin in normal human osteoblasts (NHOst) seeded on xenogenic GBO were evaluated. Our observations suggest that after four weeks of culture in differentiation medium, the NHOst showed a high affinity for the three dimensional biomaterial; in fact, cellular proliferation, migration and colonization were clearly evident. The osteogenic differentiation process, as demonstrated by morphological, histochemical, energy dispersive X-ray microanalysis and biochemical analysis was mostly obvious in the NHOst grown on threedimensional inorganic bovine bone biomaterial. Functional studies displayed a clear and significant response to calcitonin when the cells were differentiated. In addition, the presence of the biomaterial improved the response, suggesting that it could drive the differentiation of these cells towards a more differentiated osteogenic phenotype. These results encourage us to consider GBO an adequate biocompatible three-dimensional biomaterial, indicating its potential use for the development of tissue-engineering techniques.

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Tissue-engineered mucosa graft for reconstruction of the intraoral lining after freeing of the tongue: A clinical and immunohistologic study

Cited by 74 publications

References 15 publications

Comparison of Autologous Full-Thickness Gingiva and Skin Substitutes for Wound Healing

Comparison of Autologous Full-Thickness Gingiva and Skin Substitutes for Wound Healing

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Functional assay, expression of growth factors and proteins modulating bone-arrangement in human osteoblasts seeded on an anorganic bovine bone biomaterial

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