Tissue engineered human tracheas for in vivo implantation

Baiguera, Silvia; Jungebluth, Phillip; Burns, Alan J.; Mavilia, Carmelo; Haag, Johannes C.; Coppi, Paolo De; Macchiarini, Paolo

doi:10.1016/j.biomaterials.2010.08.005

Cited by 186 publications

(155 citation statements)

References 40 publications

(36 reference statements)

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“…In addition, agitation can also be used for rinsing residual detergents, by means of PBS washing cycles. It may be used for heart valves 28 , muscles 46 , peripheral nerves 47 , trachea 48 and dermis 49 .…”

Section: Agitation and Immersionmentioning

confidence: 99%

Chemical decellularization: a promising approach for preparation of extracellular matrix

Hrebíková¹,

Díaz²,

Mokrý³

2015

BIOMED PAP

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Background.A biological scaffold from extracellular matrix can be produced by a variety of decellularization methods whose caveat consists in efficiently eliminating cells from the treated tissue. This scaffold can be used in diverse applications for tissue engineering and organ regeneration. Preservation of the extracellular matrix ultrastructure is highly desirable because of its unique architecture, contained growth factors and decreased immunological response. All of these properties provide attachment sites and adequate environment for cells colonizing this scaffold, reconstituting the decellularized organ. This review briefly describes chemical decellularization methods, evaluation of these protocols and the role of ECM in tissue engineering. Conclusion. Chemical decellularization is an often used method for scaffold preparation and makes possible a wellpreserved three dimensional structure of extracellular matrix.

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Section: Agitation and Immersionmentioning

confidence: 99%

Chemical decellularization: a promising approach for preparation of extracellular matrix

Hrebíková¹,

Díaz²,

Mokrý³

2015

BIOMED PAP

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“…A lthough replacement of airways (1)(2)(3)(4)(5)(6) or urogenital tissue (7) using stem cell-based techniques has been achieved, engineering of functional contractile muscle tissue has only been partially explored (8)(9). While debates have been generated around which myogenic progenitors (satellite cells, muscle stem cells, or myoblasts) should be used, fewer studies have focused on exploring which matrix could offer a better platform for regeneration (10,11).…”

mentioning

confidence: 99%

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Fishman

Lowdell

Urbani³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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182

137

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Decellularized (acellular) scaffolds, composed of natural extracellular matrix, form the basis of an emerging generation of tissueengineered organ and tissue replacements capable of transforming healthcare. Prime requirements for allogeneic, or xenogeneic, decellularized scaffolds are biocompatibility and absence of rejection. The humoral immune response to decellularized scaffolds has been well documented, but there is a lack of data on the cellmediated immune response toward them in vitro and in vivo. Skeletal muscle scaffolds were decellularized, characterized in vitro, and xenotransplanted. The cellular immune response toward scaffolds was evaluated by immunohistochemistry and quantified stereologically. T-cell proliferation and cytokines, as assessed by flow cytometry using carboxy-fluorescein diacetate succinimidyl ester dye and cytometric bead array, formed an in vitro surrogate marker and correlate of the in vivo host immune response toward the scaffold. Decellularized scaffolds were free of major histocompatibility complex class I and II antigens and were found to exert anti-inflammatory and immunosuppressive effects, as evidenced by delayed biodegradation time in vivo; reduced sensitized T-cell proliferative activity in vitro; reduced IL-2, IFN-γ, and raised IL-10 levels in cell-culture supernatants; polarization of the macrophage response in vivo toward an M2 phenotype; and improved survival of donor-derived xenogeneic cells at 2 and 4 wk in vivo. Decellularized scaffolds polarize host responses away from a classical TH1-proinflammatory profile and appear to down-regulate T-cell xeno responses and TH1 effector function by inducing a state of peripheral T-cell hyporesponsiveness. These results have substantial implications for the future clinical application of tissue-engineered therapies.

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“…Asimismo, varias familias de moléculas, que incluyen los GAG, colágenos y glicoproteínas no colágenas, presentan propiedades físicas que promueven la invasión y migración celulares (15,16). En años recientes se ha demostrado que las matrices descelularizadas provenientes de fuentes naturales proporcionan una estructura y composición casi idénticas a su estado nativo, favoreciendo la unión celular, la migración y la interacción con el tejido del hospedero (17). En el año 2008, se publicó el reporte de caso del grupo de Macchiarini (18) relacionado con un trasplante de trá-quea utilizando el sistema mencionado; los resultados exitosos descritos por este grupo han abierto enormes posibilidades para los pacientes con alteraciones en la vía aérea.…”

Section: Discussionunclassified

“…Los esfuerzos por desarrollar matrices extracelulares derivadas de material protésico no han sido satisfactorios hasta el momento, puesto que no proporcionan las señales específicas para que se lleven a cabo las interacciones célula-matriz (17). Esto se debe en gran parte a la diversidad de componentes de la matriz extracelular, que pueden ser similares en una amplia gama de tejidos, pero cada uno contiene su propia combinación de macromoléculas que proporcionan las señales celulares necesarias (20,21).…”

Section: Discussionunclassified

Evaluación del método químico-enzimático de descelularización para la obtención de matrices extracelulares de tráquea en el modelo porcino

Morales-Valencia

Patiño-Vargas

Correa-Londoño³

et al. 2016

iatreia

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Introducción: diferentes enfermedades afectan la tráquea y deterioran la calidad de vida. La ingeniería tisular es una alternativa terapéutica para los pacientes con esas enfermedades: matrices de tráquea descelularizadas y sembradas con células del receptor no generan respuesta inmune y pueden prevenir incluso el rechazo de zootrasplantes.Objetivo: evaluar un método de descelularización para obtener matrices extracelulares de tráquea en el modelo porcino.Materiales y métodos: a partir de 5 tráqueas porcinas se formaron dos grupos de estudio, controles y tratados con un método químico-enzimático. Se hizo análisis histológico con hematoxilina-eosina, coloración tricrómica de Masson y safranina O. Se evaluaron las propiedades biomecánicas de ambos grupos, mediante la determinación del módulo de Young, la fuerza máxima y el porcentaje de deformación.Resultados: en las muestras tratadas se observó una disminución del 66 % del contenido celular en comparación con los controles. Se preservó el colágeno y se detectó reducción de los glucosaminoglucanos. Las pruebas biomecánicas revelaron una diferencia estadís-ticamente significativa del porcentaje de deformación, sin alteración de los demás pará-metros.Conclusiones: el método evaluado demostró ser eficiente para descelularizar tráqueas de cerdo, con una disminución importante en el costo y el tiempo de tratamiento, por lo que podría ser una buena opción en las condiciones socioeconómicas de Colombia. Introduction: Different diseases may affect the trachea and, therefore, the quality of life. Tissue engineering may be a therapeutic alternative for patients with such diseases, using decellularized trachea matrixes seeded with cells from the recipient, which do not generate immune response and may even prevent rejection of zoo-transplants.Objective: To evaluate a decellularization method to obtain tracheal extracellular matrixes in the porcine model. Materials and methods:Two study groups, treated and control, were obtained from 5 porcine tracheas. A chemical-enzymatic method for decellularization was used. Histological analyses were performed with hematoxylin-eosin, Masson's trichromic stain and safranin O. Biomechanical properties of both groups were evaluated by determining the Young modulus, maximum strength and deformation rate.Results: Compared to the controls, there was a 66 % decrease in the cell content in the treated specimens. Collagen was preserved and a reduction of glycosaminoglycans was detected. Biomechanical tests revealed a significant difference in the percentage of deformation, with no alteration of the remaining parameters. Conclusions:The evaluated decellularization method proved to be efficient to reduce the cellular content of porcine tracheas, with a considerable decrease in cost and production time. These advantages could make it a good option for the socio-economic Colombian conditions. KEY WORDSDecellularized Matrix; Recellularized Matrix; Regeneration, Airway; Trachea; Tissue Engineering RESUMOAvaliação do método químico-enzimático de descelu...

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Tissue engineered human tracheas for in vivo implantation

Cited by 186 publications

References 40 publications

Chemical decellularization: a promising approach for preparation of extracellular matrix

Chemical decellularization: a promising approach for preparation of extracellular matrix

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Evaluación del método químico-enzimático de descelularización para la obtención de matrices extracelulares de tráquea en el modelo porcino

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