Tissue Engineered Human Skin Equivalents

Zhang, Zheng; Michniak‐Kohn, Bozena

doi:10.3390/pharmaceutics4010026

Cited by 136 publications

(114 citation statements)

References 66 publications

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“…Skin constructs in different settings, such as epidermal (only keratinocytes) or full-thickness (keratinocytes and fibroblasts), are broadly being used in pharmaceutical and cosmetic industry to evaluate the toxicity and efficacy of drug candidates [27] . In addition, incorporating different skin components, such as macrophages [28] , melanocytes [29] and sweat glands [30] , into engineered skin constructs has enabled the development of more reliable human skin models for in vitro drug testing studies.…”

Section: Discussionmentioning

confidence: 99%

Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC‐Derived Endothelial Cells

Abaci

Guo

Coffman

et al. 2016

Adv Healthcare Materials

196

171

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Vascularization of engineered human skin constructs is crucial for recapitulation of systemic drug delivery and for their long-term survival, functionality, and viable engraftment. In this study, we used the latest microfabrication techniques and established a novel bioengineering approach to micropattern spatially-controlled and perfusable vascular networks in 3D human skin equivalents using both primary and induced pluripotent stem cell (iPSC)-derived endothelial cells (ECs). Using 3D printing technology, we were able to control the geometry of the micropatterned vascular networks. We verified that vascularized human skin equivalents (vHSEs) can form a robust epidermis and establish an endothelial barrier function, which allows for the recapitulation of both topical and systemic delivery of drugs. In addition, we examined the therapeutic potential of vHSEs for cutaneous wounds on immunodeficient mice and demonstrated that vHSEs can both promote and guide neovascularization during wound healing. Overall, this innovative bioengineering approach could enable in vitro evaluation of topical and systemic drug delivery, as well as improve the potential of engineered skin constructs to be used as a potential therapeutic option for the treatment of cutaneous wounds.

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Section: Discussionmentioning

confidence: 99%

Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC‐Derived Endothelial Cells

Abaci

Guo

Coffman

et al. 2016

Adv Healthcare Materials

196

171

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“…1). The barrier function of intact epidermis depends on the quality of the present cells and the surrounding matrix [16,17]. Epidermis and dermis are physically separated by the basement membrane or basal lamina, a highly specialized ECM structure, which provides a stabilizing and dynamic interface [18].…”

Section: Mammalian Skin and Wound Healingmentioning

confidence: 99%

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Borena

Martens

Broeckx

et al. 2015

Cell Physiol Biochem

169

112

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Mammal skin has a crucial function in several life-preserving processes such as hydration, protection against chemicals and pathogens, initialization of vitamin D synthesis, excretion and heat regulation. Severe damage of the skin may therefore be life-threatening. Skin wound repair is a multiphased, yet well-orchestrated process including the interaction of various cell types, growth factors and cytokines aiming at closure of the skin and preferably resulting in tissue repair. Regardless various therapeutic modalities targeting at enhancing wound healing, the development of novel approaches for this pathology remains a clinical challenge. The time-consuming conservative wound management is mainly restricted to wound repair rather than restitution of the tissue integrity (the so-called “restitutio ad integrum”). Therefore, there is a continued search towards more efficacious wound therapies to reduce health care burden, provide patients with long-term relief and ultimately scarless wound healing. Recent in vivo and in vitro studies on the use of skin wound regenerative therapies provide encouraging results, but more protracted studies will have to determine whether the effect of observed effects are clinically significant and whether regeneration rather than repair can be achieved. For all the aforementioned reasons, this article reviews the emerging field of regenerative skin wound healing in mammals with particular emphasis on growth factor- and stem cell-based therapies.

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“…More complex tissues, composed of combinations of two or more cell types and/or at least two "skin" layers (epidermis and dermis), forming as a result either healthy or diseased models of human skin, are also available (Bannasch et al, 2005;Semlin et al, 2011;Zhang and Michniak-Kohn, 2012;El Ghalbzouri et al, 2009;Nischt et al, 2006;Ponec et al, 2003;van den Bogaard et al, 2014) (Tab. 2b,c).…”

Section: Reconstructed In Vitro Human Skin Modelsmentioning

confidence: 99%

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

Gordon

Daneshian

Bouwstra

et al. 2015

ALTEX

122

103

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SummaryModels of the outer epithelia of the human body -namely the skin, the intestine and the lung -have found valid applications in both research and industrial settings as attractive alternatives to animal testing. A variety of approaches to model these barriers are currently employed in such fields, ranging from the utilization of ex vivo tissue to reconstructed in vitro models, and further to chip-based technologies, synthetic membrane systems and, of increasing current interest, in silico modeling approaches. An international group of experts in the field of epithelial barriers was convened from academia, industry and regulatory bodies to present both the current state of the art of non-animal models of the skin, intestinal and pulmonary barriers in their various fields of application, and to discuss research-based, industry-driven and regulatory-relevant future directions for both the development of new models and the refinement of existing test methods. Issues of model relevance and preference, validation and standardization, acceptance, and the need for simplicity versus complexity were focal themes of the discussions. The outcomes of workshop presentations and discussions, in relation to both current status and future directions in the utilization and development of epithelial barrier models, are presented by the attending experts in the current report.

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Tissue Engineered Human Skin Equivalents

Cited by 136 publications

References 66 publications

Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC‐Derived Endothelial Cells

Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC‐Derived Endothelial Cells

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

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