Tissue Engineered Epithelial Cell Sheets for the Creation of a Bioartificial Trachea

Kanzaki, Masato; Yamato, Masayuki; Hatakeyama, Hideyuki; Kohno, Chinatsu; Yang, Joseph; Umemoto, Terumasa; Kikuchi, Akihiko; Okano, Teruo; Onuki, Takamasa

doi:10.1089/ten.2006.12.1275

Cited by 81 publications

(31 citation statements)

References 26 publications

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“…However, the only function of these rings is the prevention of the tube's collapse, so the main requirements of the replacement are purely mechanical. But, the necessity of the epithelial layer inside imposes a vascular support beneath, thus the integration of the implant with the vasculature of the body becomes an important determinant [24]. The open porous structure of titanium is excellent for tissue ingrowth, but it lacks the ability to control the extent of the ingrowth.…”

Section: Discussionmentioning

confidence: 99%

Hybrid Titanium/Biodegradable Polymer Implants with an Hierarchical Pore Structure as a Means to Control Selective Cell Movement

et al. 2011

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In order to improve implant success rate, it is important to enhance their responsiveness to the prevailing conditions following implantation. Uncontrolled movement of inflammatory cells and fibroblasts is one of these in vivo problems and the porosity properties of the implant have a strong effect on these. Here, we describe a hybrid system composed of a macroporous titanium structure filled with a microporous biodegradable polymer. This polymer matrix has a distinct porosity gradient to accommodate different cell types (fibroblasts and epithelial cells). The main clinical application of this system will be the prevention of restenosis due to excessive fibroblast migration and proliferation in the case of tracheal implants.Methodology/Principal FindingsA microbead-based titanium template was filled with a porous Poly (L-lactic acid) (PLLA) body by freeze-extraction method. A distinct porosity difference was obtained between the inner and outer surfaces of the implant as characterized by image analysis and Mercury porosimetry (9.8±2.2 µm vs. 36.7±11.4 µm, p≤0.05). On top, a thin PLLA film was added to optimize the growth of epithelial cells, which was confirmed by using human respiratory epithelial cells. To check the control of fibroblast movement, PKH26 labeled fibroblasts were seeded onto Titanium and Titanium/PLLA implants. The cell movement was quantified by confocal microscopy: in one week cells moved deeper in Ti samples compared to Ti/PLLA.ConclusionsIn vitro experiments showed that this new implant is effective for guiding different kind of cells it will contact upon implantation. Overall, this system would enable spatial and temporal control over cell migration by a gradient ranging from macroporosity to nanoporosity within a tracheal implant. Moreover, mechanical properties will be dependent mainly on the titanium frame. This will make it possible to create a polymeric environment which is suitable for cells without the need to meet mechanical requirements with the polymeric structure.

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Section: Discussionmentioning

confidence: 99%

Hybrid Titanium/Biodegradable Polymer Implants with an Hierarchical Pore Structure as a Means to Control Selective Cell Movement

et al. 2011

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“…In order to achieve these results, many cell types have been used alone or in combination including chondrocytes, epithelial cells, fibroblasts, adipose tissue, chondrocytes, mesenchymal amniocytes, and mesenchymal stem cells (bone-marrow mononuclear cells). 17,20,26–28,34,38,48–58 …”

Section: Discussionmentioning

confidence: 99%

Clinical Translation of Tissue Engineered Trachea Grafts

Chiang

Pepper

Best

et al. 2016

Ann Otol Rhinol Laryngol

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Objective To provide a state-of-the-art review discussing recent achievements in tissue engineered tracheal reconstruction. Data Sources and Review Methods A structured PubMed search of the current literature up to and including October 2015. Representative articles that discuss the translation of tissue engineered tracheal grafts (TETG) were reviewed. Conclusions The integration of a biologically compatible support with autologous cells has resulted in successful regeneration of respiratory epithelium, cartilage, and vascularization with graft patency, although the optimal construct composition has yet to be defined. Segmental TETG constructs are more commonly complicated by stenosis and delayed epithelialization when compared to patch tracheoplasty. Implications for Practice The recent history of human TETG recipients represents revolutionary proof of principle studies in regenerative medicine. Application of TETG remains limited to a compassionate use basis; however, defining the mechanisms of cartilage formation, epithelialization, and refinement of in vivo regeneration will advance the translation of TETG from the bench to the bedside.

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“…A straight Dacron prosthesis, reinforced with spiral-shaped polypropylene monofilaments, has been firstly implanted subcutaneously into rabbits, to allow the infiltration of blood vessels and connective tissue, and then was vertically opened and conditioned, on the inner surface, with tracheal epithelial cell sheets. The resulting bioartificial trachea was implanted (3 cm length): after 1 month a mature columnar epithelium was revealed in the interior graft portions, highlighting the importance of a functional epithelium lining the lumen of a prosthetic trachea [40]. …”

Section: Tracheamentioning

confidence: 99%

Tissue Engineered Scaffolds for an Effective Healing and Regeneration: Reviewing Orthotopic Studies

Baiguera

Urbani

Gaudio

2014

BioMed Research International

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It is commonly stated that tissue engineering is the most promising approach to treat or replace failing tissues/organs. For this aim, a specific strategy should be planned including proper selection of biomaterials, fabrication techniques, cell lines, and signaling cues. A great effort has been pursued to develop suitable scaffolds for the restoration of a variety of tissues and a huge number of protocols ranging from in vitro to in vivo studies, the latter further differentiating into several procedures depending on the type of implantation (i.e., subcutaneous or orthotopic) and the model adopted (i.e., animal or human), have been developed. All together, the published reports demonstrate that the proposed tissue engineering approaches spread toward multiple directions. The critical review of this scenario might suggest, at the same time, that a limited number of studies gave a real improvement to the field, especially referring to in vivo investigations. In this regard, the present paper aims to review the results of in vivo tissue engineering experimentations, focusing on the role of the scaffold and its specificity with respect to the tissue to be regenerated, in order to verify whether an extracellular matrix-like device, as usually stated, could promote an expected positive outcome.

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Tissue Engineered Epithelial Cell Sheets for the Creation of a Bioartificial Trachea

Cited by 81 publications

References 26 publications

Hybrid Titanium/Biodegradable Polymer Implants with an Hierarchical Pore Structure as a Means to Control Selective Cell Movement

Hybrid Titanium/Biodegradable Polymer Implants with an Hierarchical Pore Structure as a Means to Control Selective Cell Movement

Clinical Translation of Tissue Engineered Trachea Grafts

Tissue Engineered Scaffolds for an Effective Healing and Regeneration: Reviewing Orthotopic Studies

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