Tissue-Engineered Composites of Anulus Fibrosus and Nucleus Pulposus for Intervertebral Disc Replacement

Mizuno, Hirokazu; Roy, Amit; Vacanti, Charles A.; Kojima, Koji; Ueda, Minoru; Bonassar, Lawrence J.

doi:10.1097/01.brs.0000128264.46510.27

Cited by 208 publications

(169 citation statements)

References 28 publications

Supporting

Mentioning

168

Contrasting

Order By: Relevance

“…AF tissue engineering approaches have evaluated AF cells seeded in several types of 3D hydrogels or scaffolds [7,[14][15][16][17][18]. These studies have demonstrated that AF cells proliferate in 3D culture and synthesize ECM, reconstituting the histological structure of native tissue.…”

Section: Introductionmentioning

confidence: 99%

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

et al. 2012

View full text Add to dashboard Cite

Purpose Annulus fibrosus (AF) tissue engineering is gathering increasing interest for the development of strategies to reduce recurrent disc herniation (DH) rate and to increase the effectiveness of intervertebral disc regeneration strategies. This study evaluates the use of a bioactive microfibrous poly(L-lactide) scaffold releasing Transforming Growth Factor (TGF)-b1 (PLLA/TGF) for the repair and regeneration of damaged AF. Methods The scaffold was synthesized by electrospinning, with a direct incorporation of TGF-b1 into the polymeric solution, and characterized in terms of morphology and drug release profile. Biological evaluation was performed with bovine AF cells (AFCs) that were cultured on the scaffold up to 3 weeks to quantitatively assess glycosaminoglycans and total collagen production, using bare electrospun PLLA as a control. Histological evaluation was performed to determine the thickness of the deposited neo-ECM. Results Results demonstrated that AFCs cultured on PLLA/TGF deposited a significantly greater amount of glycosaminoglycans and total collagen than the control, with higher neo-ECM thickness. Conclusions PLLA/TGF scaffold induced an anabolic stimulus on AFCs, mimicking the ECM three-dimensional environment of AF tissue. This bioactive scaffold showed encouraging results that allow envisaging an application for AF tissue engineering strategies and AF repair after discectomy for the prevention of recurrent DH.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Reviews for nucleus replacements have been reported elsewhere [29,103]. On the other hand, biomaterials used for annulus replacement are dominated by synthetic biomaterials including silk [23], polycaprolactone [80] and its derivatives [118], polyglycolic acid/ polylactic acid [76] and bioglass [120] with a few natural biomaterials such as collagen/hyaluronan [2]. In most if not all cases, freeze-drying has been employed as the fabrication method to create porous structures.…”

Section: Scaffolding In Intervertebral Disc Tissue Engineeringmentioning

confidence: 99%

“…A wide range of biomaterials dominated by natural biomaterials has been used for nucleus replacement. These materials include collagen [121], atellocollagen [98,99], alginate [70,76], gelatin [118], chitosan [33], collagen/ glycosaminoglycan [97], collagen/hyaluronan [2] and poly-L-lactic acid [94]. Reviews for nucleus replacements have been reported elsewhere [29,103].…”

Section: Scaffolding In Intervertebral Disc Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

View full text Add to dashboard Cite

Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

show abstract

“…As a result, strategies addressing the different components of IVD structure, NP, and AF, could be required to restore IVD functions. As shown in Table 1, few studies have shown the efficacy of synthetic and natural polymers to regenerate AF and NP (Mizuno et al, 2004;Hamilton et al, 2005;Mizuno et al, 2006;Nerurkar et al, 2008;Nesti et al, 2008). However, a study performed by Mizuno et al (Mizuno et al, 2004) revealed the efficacy of an integrated biphasic composite for AF and NP regeneration.…”

Section: Polymeric-based Designsmentioning

confidence: 99%

“…The three main components of IVD aimed at tissue regeneration are: 1) AF (Sato et al, 2003;Chang et al, 2007;Helen and Gough, 2007;Nerurkar et al, 2007;Shao and Hunter, 2007;Wan et al, 2007;Nerurkar et al, 2008;Wang et al, 2008;Nerurkar et al, 2009); 2) NP (Baer et al, 2001;Sakai et al, 2003;Seguin et al, 2004;Bertram et al, 2005;Wilke et al, 2006;Roughley et al, 2006;Cloyd et al, 2007;Chou et al, 2008;Halloran et al, 2008;Richardson et al, 2008;Vernengo et al, 2008;Bron et al, 2009;Chou et al, 2009;Sawamura et al, 2009;Calderon et al, 2010;Silva-Correia et al, 2010a;Silva-Correia et al, 2010b;Reza and Nicoll, 2010;); and 3) both NP and AF by means of using biphasic scaffolds (Mizuno et al, 2004;Hamilton et al, 2005;Mizuno et al, 2006;Nerurkar et al, 2008;Nesti et al, 2008). It has been proposed that for producing a tissue resembling natural IVD, total IVD replacement could be required.…”

Section: Polymeric-based Designsmentioning

confidence: 99%

Intervertebral Disc Regeneration

Libera¹,

Hoell

Holzhausen³

et al.

Fundamentals of Tissue Engineering and Regenerative Medicine

View full text Add to dashboard Cite

Low back pain is an extremely common illness syndrome that causes patient suffering and disability and requires urgent solutions to improve the quality of life of these patients. Treatment options aimed to regenerate the intervertebral disc (IVD) are still under development. The cellular complexity of IVD, and consequently its fine regulatory system, makes it a challenge to the scientific community. Biomaterials-based therapies are the most interesting solutions to date, whereby tissue engineering and regenerative medicine (TE&RM) strategies are included. By using such strategies, i.e., combining biomaterials, cells, and biomolecules, the ultimate goal of reaching a complete integration between native and neo-tissue can be achieved. Hydrogels are promising materials for restoring IVD, mainly nucleus pulposus (NP). This study presents an overview of the use of hydrogels in acellular and cellular strategies for intervertebral disc regeneration. To better understand IVD and its functioning, this study will focus on several aspects: anatomy, pathophysiology, cellular and biomolecular performance, intrinsic healing processes, and current therapies. In addition, the application of hydrogels as NP substitutes will be addressed due to their similarities to NP mechanical properties and extracellular matrix. These hydrogels can be used in cellular strategies when combined with cells from different sources, or in acellular strategies by performing the functionalization of the hydrogels with biomolecules. In addition, a brief summary of therapies based on simple injection for primary biological repair will be examined. Finally, special emphasis will focus on reviewing original studies reporting on the use of autologous cells and biomolecules such as platelet-rich plasma and their potential clinical applications.

show abstract

Tissue-Engineered Composites of Anulus Fibrosus and Nucleus Pulposus for Intervertebral Disc Replacement

Abstract: These results demonstrate the feasibility of creating a composite intervertebral disc with both anulusfibrosus and nucleus pulposus for clinical applications.

Cited by 208 publications

References 28 publications

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Intervertebral Disc Regeneration

Contact Info

Product

Resources

About