Tissue engineered cartilage “bioshell” protective layer for subcutaneous implants

Monroy, Angelo; Kojima, Koji; Ghanem, Marcielle A.; Paz, Ana C.; Kamil, Syed H.; Vacanti, Charles A.; Eavey, Roland D.

doi:10.1016/j.ijporl.2006.11.025

Cited by 19 publications

(10 citation statements)

References 15 publications

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“…Several studies have examined chondrocyte behaviour in one type of hydrogel. Monroy et al (2007) and Saim et al (2000) examined chondrocyte behaviour in the temperature-sensitive gel, Pluronic F127. They demonstrated that Pluronic F127 could be successfully used to encapsulate chondrocytes and facilitate in vivo cartilage formation.…”

Section: Introductionmentioning

confidence: 99%

The composition of hydrogels for cartilage tissue engineering can influence glycosaminoglycan profile

Wang

Hughes²,

Cartmell³

et al. 2010

eCM

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The injectable and hydrophilic nature of hydrogels makes them suitable candidates for cartilage tissue engineering. To date, a wide range of hydrogels have been proposed for articular cartilage regeneration but few studies have quantitatively compared chondrocyte behaviour and extracellular matrix (ECM) synthesis within the hydrogels. Herein we have examined the nature of ECM synthesis by chondrocytes seeded into four hydrogels formed by either temperature change, self-assembly or chemical crosslinking. Bovine articular cartilage chondrocytes were cultured for 14 days in Extracel®, Pluronic F127 blended with Type II collagen, Puramatrix® and Matrixhyal®. The discriminatory and sensitive technique of fluorophoreassisted carbohydrate electrophoresis (FACE) was used to determine the fine detail of the glycosaminoglycans (GAG); hyaluronan and chondroitin sulphate. FACE analysis for chondroitin sulphate and hyaluronan profiles in Puramatrix® closely matched that of native cartilage. For each hydrogel, DNA content, viability and morphology were assessed. Total collagen and total sulphated GAG production were measured and normalised to DNA content. Significant differences were found in total collagen synthesis. By day 14, Extracel® and Puramatrix® had significantly more total collagen than Matrixhyal® (1.77±0.26 μg and 1.97±0.26 μg vs. 0.60±0.26 μg; p<0.05). sGAG synthesis occurred in all hydrogels but a significantly higher amount of sGAG was retained within Extracel® at days 7 and 14 (p<0.05). In summary, we have shown that the biochemical and biophysical characteristics of each hydrogel directly or indirectly influenced ECM formation. A detailed understanding of the ECM in the development of engineered constructs is an important step in monitoring the success of cartilage regeneration strategies.

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Section: Introductionmentioning

confidence: 99%

The composition of hydrogels for cartilage tissue engineering can influence glycosaminoglycan profile

Wang

Hughes²,

Cartmell³

et al. 2010

eCM

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“…8,20,35 However, most of the auricular cartilage studies use large amounts of tissue obtained from experimental animals, which is not practical for clinical application where the harvested specimen size should ideally be limited to avoid donor site morbidity. 9,10,[36][37][38][39] Although the perichondrium possesses cartilage progenitor cells, the low yield of "neocartilage" subsequently, and influence of patient factors, such as age, has limited its application. 1,24,[28][29][30] We were able to isolate more cells from cartilage samples harvested with perichondrium, compared with cartilage samples harvested alone.…”

Section: Discussionmentioning

confidence: 99%

A Novel Biodegradable Polyurethane Matrix for Auricular Cartilage Repair

Iyer

Dearman

Wagstaff

et al. 2016

Journal of Burn Care & Research

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Auricular reconstruction poses a challenge for reconstructive and burns surgeons. Techniques involving cartilage tissue engineering have shown potential in recent years. A biodegradable polyurethane matrix developed for dermal reconstruction offers an alternative to autologous, allogeneic, or xenogeneic biologicals for cartilage reconstruction. This study assesses such a polyurethane matrix for this indication in vivo and in vitro. To evaluate intrinsic cartilage repair, three pigs underwent auricular surgery to create excisional cartilage ± perichondrial defects, measuring 2 × 3 cm in each ear, into which acellular polyurethane matrices were implanted. Biopsies were taken at day 28 for histological assessment. Porcine chondrocytes ± perichondrocytes were cultured and seeded in vitro onto 1 × 1 cm polyurethane scaffolds. The total culture period was 42 days; confocal, histological, and immunohistochemical analyses of scaffold cultures were performed on days 14, 28, and 42. In vivo, the polyurethane matrices integrated with granulation tissue filling all biopsy samples. Minimal neocartilage invasion was observed marginally on some samples. Tissue composition was identical between ears whether perichondrium was left intact, or not. In vitro, the polyurethane matrix was biocompatible with chondrocytes ± perichondrocytes and supported production of extracellular matrix and Type II collagen. No difference was observed between chondrocyte culture alone and chondrocyte/perichondrocyte scaffold coculture. The polyurethane matrix successfully integrated into the auricular defect and was a suitable scaffold in vitro for cartilage tissue engineering, demonstrating its potential application in auricular reconstruction.

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“…Multiple strategies are being tested for optimization of mesh–surface properties to reduce deletirious tissue–mesh reactions . Researchers have attempted to grow certain cells on medical scaffolds to improve biocompatibility and graft function . Several attempts have also been made to cell‐coat surgical meshes for hernia repair .…”

Section: Discussionmentioning

confidence: 99%

“…9,10, [14][15][16] Researchers have attempted to grow certain cells on medical scaffolds to improve biocompatibility and graft function. [17][18][19][20][21][22][23] Several attempts have also been made to cell-coat surgical meshes for hernia repair. [24][25][26] It is clear from these studies, that the methodology for cell coating, the growth dynamics of cells on surgical meshes, and the "amenability" to cell coating of various meshes was yet to be optimized.…”

Section: Discussionmentioning

confidence: 99%

Methodology of fibroblast and mesenchymal stem cell coating of surgical meshes: A pilot analysis

et al. 2013

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Coating of various synthetic, absorbable, and biologic meshes with mesenchymal stem cells (MSCs) and fibroblasts was analyzed qualitatively and quantitatively. Five hernia meshes-light weight monofilament polypropylene (Soft Mesh), polyester (Parietex-TET), polylactide composite (TIGR), heavy weight monofilament polypropylene (Marlex), and porcine dermal collagen (Strattice)-were coated with three cell lines: human dermal fibroblasts (HFs), rat kidney fibroblasts (NRKs), and rat MSCs. Cell densities were determined at different time points. Samples also underwent histology and transmission electron microscopic (TEM) analyses. It required HFs 3 weeks to cover the entire mesh, while only 2 weeks for NRKs and MSCs to do so. MSCs had no preference for any of the meshes and produced the highest cell densities on Parietex and TIGR. Substrate-preference accounted for the significantly lower fibroblast densities on TIGR than Parietex. Fibroblasts failed to coat Marlex. Strattice, which had the least surface area, generated comparable cell densities to Parietex. Both histology and TEM confirmed cell coating of mesh surface. Various prosthetics can be coated by certain cell strains. Both mesh composition and cell preference dramatically influence the coating process. This methodology provides foundation for novel avenues of modulation of host response to various modern synthetic and biologic meshes.

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Tissue engineered cartilage “bioshell” protective layer for subcutaneous implants

Cited by 19 publications

References 15 publications

The composition of hydrogels for cartilage tissue engineering can influence glycosaminoglycan profile

The composition of hydrogels for cartilage tissue engineering can influence glycosaminoglycan profile

A Novel Biodegradable Polyurethane Matrix for Auricular Cartilage Repair

Methodology of fibroblast and mesenchymal stem cell coating of surgical meshes: A pilot analysis

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