Tissue engineered bulking agent with adipose-derived stem cells and silk fibroin microspheres for the treatment of intrinsic urethral sphincter deficiency

Shi, Li; Cai, Huixia; Chen, Long Kun; Wu, Yan; Zhu, Shou An; Gong, Xiao; Xia, Ya Xian; Ouyang, Hong; Zou, Xiao Hui

doi:10.1016/j.biomaterials.2013.11.025

Cited by 63 publications

(38 citation statements)

References 42 publications

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“…In generally, there are two subtypes of SUI: urethral hypermobility caused by anatomic lesions and typically a loss of backstop support at the bladder neck, and intrinsic sphincter deficiency (ISD) due to the weakness of the urethral sphincter [2]. A decrease in estrogen levels is a characteristic and important feature of postmenopausal women, which may be associated with ISD [3].…”

Section: Introductionmentioning

confidence: 99%

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Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

Feng

Liu

et al. 2016

PLoS ONE

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The increased incidence of stress urinary incontinence (SUI) in postmenopausal women has been proposed to be associated with a reduction in the level of 17-β estradiol (E2). E2 has also been shown to enhance the multi-differentiation ability of adipose-derived stem cells (ASCs) in vitro. However, studies on the potential value of E2 for tissue engineering in SUI treatment are rare. In the present study, we successfully fabricated myogenically differentiated ASCs (MD-ASCs), which were seeded onto a Poly(l-lactide)/Poly(e-caprolactone) electrospinning nano-scaffold, and incorporated E2 into the system, with the aim of improving the proliferation and myogenic differentiation of ASCs. ASCs were collected from the inguinal subcutaneous fat of rats. The proliferation and myogenic differentiation of ASCs, as well as the nano-scaffold biocompatibility of MD-ASCs, with or without E2 supplementation, were investigated. We demonstrated that E2 incorporation enhanced the proliferation of ASCs in vitro, and the most optimal concentration was 10−9 M. E2 also led to modulation of the MD-ASCs phenotype toward a concentrated type with smooth muscle-inductive medium. The expression of early (alpha-smooth muscle actin), mid (calponin), and late-stage (myosin heavy chain) contractile markers in MD-ASCs was enhanced by E2 during the different differentiation stages. Furthermore, the nano-scaffold was biocompatible with MD-ASCs, and cell proliferation was significantly enhanced by E2. Taken together, these results demonstrate that E2 can enhance the proliferation and myogenic differentiation of ASCs and can be used to construct a biocompatible cell/nano-scaffold. These scaffolds with desirable differentiation cells show promising applications for tissue engineering.

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Section: Introductionmentioning

confidence: 99%

“…However, the cells can become injured and experience uncontrollable death during implanting, which negatively affects the therapy outcome [2]. Tissue engineering is a newly emerging strategy that shows potential for the treatment of ISD.…”

Section: Introductionmentioning

confidence: 99%

Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

Feng

Liu

et al. 2016

PLoS ONE

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“…В настоящее время зарубежные группы ученых пред-лагают использовать хирургические сетки, получаемые на основе природных биополимеров (из регенерированного фиброина шелка Bombyx mori, шелка паутинной нити, из белка 1F9 -аналога спидроина1 Nephila clavipes) [16][17][18] в силу того, что на них возможно образование монослоя из живых, способных к быстрой регенерации мезенхи-мальных стволовых клеток более быстрое, чем на синте-тических хирургических сетках [19]. Однако к недостат-кам биополимеров относят высокую стоимость их полу-чения, сложность обработки, недостаточную механиче-скую прочность.…”

Section: Discussionunclassified

Experience with a cell engineering construction for the surgical treatment of pelvic prolapse: A preliminary communication

Люндуп¹,

Сулина²,

Ishchenko³

et al. 2015

Ross. vestn. akush.-ginekol.

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Одной из важнейших проблем гинекологии является пролапс гениталий -патология, достигающая 34,1-56,3% среди женской популяции некоторых стран [1]. По данным отечественных авторов [2][3][4], частота пролапса гениталий колеблется от 1,7 до 38,9% и среди гинекологи-ческих заболеваний составляет 28-38,9%, около 15% от так называемых больших гинекологических операций. В НОВЫЕ ТЕХНОЛОГИИ © Коллектив авторов, 2015 e-mail: ya.suli.na@gmail.com структуре плановых показаний к оперативному лечению выпадение матки и влагалища занимает 3-е место после доброкачественных опухолей и эндометриоза [5,6]. Осо-бую актуальность проблема пролапса гениталий приобре-тает ввиду тенденции к «омоложению» этой патологии, сопровождаемой тяжелыми дисфункциональными изме-нениями соседних органов. У 85,5% больных развиваются Цель исследования -создание новой клеточно-инженерной конструкции для усовершенствования имплантационных материалов, применяемых для восстановления фасциальных дефектов тазового дна у пациенток с различными видами пролапса гениталий. Материал и методы. Проводили забор образцов кожи размером 2×3×1 мм у здорового добровольца для получения культуры дермальных фибробластов с помощью специальных сред. В качестве матрикса использовались сетчатые имплантаты, применяемые при хирургическом лечении пролапса гениталий, на образцы которых наносились дермальные фибробласты человека третьего пассажа. Комплексы сеток с клетками инкубировались во влажной среде при температуре 37 °С и проводилась их микроскопия. Результаты. Начало закрытия ячеек клетками начиналось на 10-14-е сутки после посева клеток на сетку. Закрытие большей части ячеек многослойной клеточной структурой наблюдали к 30-м суткам, а закрытие всех ячеек сетки -на 60-е сутки культивирования. Заключение. Предложенная клеточно-инженерная конструкция, основанная на синтезе коллагена и прорастании фиброзной ткани на сетчатых имплантатах, может приве-сти к более прочному соединению сетки с окружающими тканями по сравнению с синтетическими протезами и улучшить результаты хирургического лечения.Авторы информируют об отсутствии конфликта интересов. Ключевые слова: хирургическое лечение тазового пролапса, клеточно-инженерная конструкция, сетчатый имплантат, дермальные фибробласты человека, биосовместимые матрицы, тазовый пролапс.Objective -to create a novel cell engineering construction to improve implantation materials used to restore fascial defects of the pelvic floor in patients with different types of genital prolapse. Material and methods. 2×3×1-mm skin samples were taken from a healthy volunteer to have dermal fibroblasts cultured in special media. Mesh implants for genital organ prolapse surgery, the samples of which third-passage human dermal fibroblasts were applied to, were used as a matrix. A set of meshes with cells were incubated under humid conditions at 37 ºC and microscopically examined. Results. Closure of wells with cells started on days 10-14 after their inoculation on the mesh. Closure of the bulk of the wells with a multilayer cellular structure was observed ...

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“…This resulted in long-term structural and functional restoration of the sphincter muscle content over 12 months to those of non-injured levels. Furthermore, Shi et al [25] injected adipose-derived stem cells combined with a silk fibroin microsphere scaffold peri-urethrally in pudendal denervated rat models. The group showed increased leak point pressures (LPPs) over 12 weeks for models treated with tissue engineered silk microspheres compared with microspheres alone.…”

Section: Regenerative Medicine Approachesmentioning

confidence: 98%

Considerations in the modern management of stress urinary incontinence resulting from intrinsic sphincter deficiency

2015

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Tissue engineered bulking agent with adipose-derived stem cells and silk fibroin microspheres for the treatment of intrinsic urethral sphincter deficiency

Cited by 63 publications

References 42 publications

Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering

Experience with a cell engineering construction for the surgical treatment of pelvic prolapse: A preliminary communication

Considerations in the modern management of stress urinary incontinence resulting from intrinsic sphincter deficiency

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