Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors

Abstract

Taken together, the use of decellularized bioscaffold and subcutaneous MSCs seems to be a potential approach to obtain bioengineered blood vessels, in the presence of EIGF supplementation.

Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds

“…1C), showing previously described morphologic characteristics of MSCs, such as fibroblastic-shape, well defined nucleus, and presence of cytoplasmic projections (Fig. 1D) [11].…”

“…Upon reaching 80% confluence, the monolayers were detached from culture wells with 0.25% trypsin/ethylenediaminetetraacetic acid (Invitrogen™) and seeded at 75 cm 2 culture flasks (Nunc™, Roskilde, Denmark). After two additional trypsin exposures, cells were cryopreserved with ice-cold FBS supplemented with 10% dimethyl sulfoxide and stored in liquid nitrogen [11].…”

“…To this purpose, however, in a heterologous approach, these scaffolds need to be decellularized to prevent immune rejections. Previous reports suggest that scaffolds derived from decellularized tissues have a facilitating role in the differentiation and obtainment of the expected tissue, as they contain the bioactive molecules and ligands necessary for stem cell maturation and diffusion of nutrients, as well as the naturally occurring 3D tissue architecture [11,33].…”

Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold

“…The initial challenge is to produce a biocompatible scaffold that will provide a microenvironment that imitates natural in vivo tissue architecture for the seeded cells 5,6 . Furthermore, the scaffolds can accomplish many other functions, including facilitating attachment, migration, proliferation, and cell interaction 7 .…”

Perspectivas de uso de células-tronco em cirurgia vascular

“…In parallel with what is being published in the literature, our team is investigating whether the scaffold obtained by decellularization of veins offers advantages over synthetic scaffolds, primarily in terms of mechanical strength. 14,15 In the future, as the experimental research is transposed to applied research in humans, we believe that it might be simpler to apply the methods in models that will use the great saphenous vein as a basis. These veins could be obtained from multiple organ donors, which would enable us to work with long segments, simulating a very natural vascular environment that is well suited to accommodate autologous MSCs and promote their differentiation into endothelium and smooth muscle.…”