Tissue-Engineered Allograft Intervertebral Disc Transplantation for the Treatment of Degenerative Disc Disease: Experimental Study in a Beagle Model

Xin, Hongkui; Zhang, Chao; Wang, Deli; Shi, Zhiyuan; Gu, Tao; Wang, Chaofeng; Wu, Jianhong; Zhang, Yan; He, Qing; Ruan, Dike

doi:10.1089/ten.tea.2012.0255

Cited by 26 publications

(24 citation statements)

References 30 publications

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“…9 Briefly, NP of intervertebral disc was collected carefully to avoid other tissues. After washing twice with phosphate-buffered saline (PBS), the NP was cut into 1 mm 2 pieces and digested overnight with 0.025% collagenase II (Sigma-Aldrich) solution in a serumfree medium at 37°C with 5% CO 2 .…”

Section: Raav-htert Modified Npcs Preparationmentioning

confidence: 99%

Intervention of rAAV-hTERT-Transducted Nucleus Pulposus Cells in Early Stage of Intervertebral Disc Degeneration: A Study in Canine Model

Shi

Xin

et al. 2015

Tissue Engineering Part A

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Section: Raav-htert Modified Npcs Preparationmentioning

confidence: 99%

Intervention of rAAV-hTERT-Transducted Nucleus Pulposus Cells in Early Stage of Intervertebral Disc Degeneration: A Study in Canine Model

Shi

Xin

et al. 2015

Tissue Engineering Part A

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“…Tissue engineered disc replacements are promising alternatives to current surgical approaches (Hudson et al, 2013). Engineered discs can potentially restore both the native structure and function while preserving the capacity of the disc to remodel in response to external stimuli (Bowles et al, 2012; Hudson et al, 2013; Lee et al, 2012; Mizuno et al, 2006; Nerurkar et al, 2010b; Xin et al, 2013). To provide a similar function compared to native intervertebral disc, tissue engineered replacements must have similar mechanical behavior as healthy intervertebral discs (Nerurkar et al, 2010a).…”

Section: Introductionmentioning

confidence: 99%

Elastic, permeability and swelling properties of human intervertebral disc tissues: A benchmark for tissue engineering

Cortés

Jacobs

DeLucca

et al. 2014

Journal of Biomechanics

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SUMMARY The aim of functional tissue engineering is to repair and replace tissues that have a biomechanical function, i.e., connective orthopaedic tissues. To do this, it is necessary to have accurate benchmarks for the elastic, permeability, and swelling (i.e., biphasic-swelling) properties of native tissues. However, in the case of the intervertebral disc, the biphasic-swelling properties of individual tissues reported in the literature exhibit great variation and even span several orders of magnitude. This variation is probably caused by differences in the testing protocols and the constitutive models used to analyze the data. Therefore, the objective of this study was to measure the human lumbar disc annulus fibrosus (AF), nucleus pulposus (NP), and cartilaginous endplates (CEP) biphasic-swelling properties using a consistent experimental protocol and analyses. The testing protocol was composed of a swelling period followed by multiple confined compression ramps. To analyze the confined compression data, the tissues were modeled using a biphasic-swelling model, which augments the standard biphasic model through the addition of a deformation-dependent osmotic pressure term. This model allows considering the swelling deformations and the contribution of osmotic pressure in the analysis of the experimental data. The swelling stretch was not different between the disc regions (AF: 1.28±0.16; NP: 1.73±0.74; CEP: 1.29±0.26), with a total average of 1.42. The aggregate modulus (Ha) of the matrix was higher in the CEP (390 kPa) compared to the NP (100 kPA) or AF (30 kPa). The permeability was very different across tissues regions, with the AF permeability (80 E−4 mm4/Ns) higher than the NP and CEP (6-7 E−16 m4/Ns). Additionally, a normalized time-constant (3000 sec) for the stress relaxation was similar for all the disc tissues. The properties measured in this study are important as benchmarks for tissue engineering and for modeling the disc's mechanical behavior and transport.

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“…Therefore, a more sensitive technique is needed to quantify the biochemical changes that occur during the early stages of IVD degeneration. Such a method would allow more accurate evaluation of new therapies for the treatment of IVD degeneration, such as injection of recombinant growth factor, gene therapy, stem cell therapy, and tissue engineering strategies [8]–[10].…”

Section: Introductionmentioning

confidence: 99%

Quantitative T2 Magnetic Resonance Imaging Compared to Morphological Grading of the Early Cervical Intervertebral Disc Degeneration: An Evaluation Approach in Asymptomatic Young Adults

et al. 2014

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ObjectiveThe objective of this study was to evaluate the efficacy of quantitative T2 magnetic resonance imaging (MRI) for quantifying early cervical intervertebral disc (IVD) degeneration in asymptomatic young adults by correlating the T2 value with Pfirrmann grade, sex, and anatomic level.MethodsSeventy asymptomatic young subjects (34 men and 36 women; mean age, 22.80±2.11 yr; range, 18–25 years) underwent 3.0-T MRI to obtain morphological data (one T1-fast spin echo (FSE) and three-plane T2-FSE, used to assign a Pfirrmann grade (I–V)) and for T2 mapping (multi-echo spin echo). T2 values in the nucleus pulposus (NP, n = 350) and anulus fibrosus (AF, n = 700) were obtained. Differences in T2 values between sexes and anatomic level were evaluated, and linear correlation analysis of T2 values versus degenerative grade was conducted.FindingsCervical IVDs of healthy young adults were commonly determined to be at Pfirrmann grades I and II. T2 values of NPs were significantly higher than those of AF at all anatomic levels (P<0.000). The NP, anterior AF and posterior AF values did not differ significantly between genders at the same anatomic level (P>0.05). T2 values decreased linearly with degenerative grade. Linear correlation analysis revealed a strong negative association between the Pfirrmann grade and the T2 values of the NP (P = 0.000) but not the T2 values of the AF (P = 0.854). However, non-degenerated discs (Pfirrmann grades I and II) showed a wide range of T2 relaxation time. T2 values according to disc degeneration level classification were as follows: grade I (>62.03 ms), grade II (54.60–62.03 ms), grade III (<54.60 ms).ConclusionsT2 quantitation provides a more sensitive and robust approach for detecting and characterizing the early stage of cervical IVD degeneration and to create a reliable quantitative in healthy young adults.

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Tissue-Engineered Allograft Intervertebral Disc Transplantation for the Treatment of Degenerative Disc Disease: Experimental Study in a Beagle Model

Cited by 26 publications

References 30 publications

Intervention of rAAV-hTERT-Transducted Nucleus Pulposus Cells in Early Stage of Intervertebral Disc Degeneration: A Study in Canine Model

Intervention of rAAV-hTERT-Transducted Nucleus Pulposus Cells in Early Stage of Intervertebral Disc Degeneration: A Study in Canine Model

Elastic, permeability and swelling properties of human intervertebral disc tissues: A benchmark for tissue engineering

Quantitative T2 Magnetic Resonance Imaging Compared to Morphological Grading of the Early Cervical Intervertebral Disc Degeneration: An Evaluation Approach in Asymptomatic Young Adults

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