Tissue Doppler and strain imaging of left ventricle in Beagle dogs with iatrogenic hypercortisolism

Oui, Heejin; Jeon, Sunghoon; Lee, Gahyun; Park, Seungjo; Cho, Kyoung-Oh; Choi, Jihye

doi:10.4142/jvs.2015.16.3.357

Cited by 4 publications

(8 citation statements)

References 26 publications

(38 reference statements)

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“…LVH and fibrosis are also caused by systemic hypertension, a common complication in CS [ 2 , 15 ]. None of the conventional echocardiographic results correlated with SBP, which is consistent with previous reports of no relationship between LVFW thickness and systolic blood pressure in canine CS patients [ 22 , 44 ].…”

Section: Discussionsupporting

confidence: 90%

“…Furthermore, cardiomyocyte hypertrophy and increased myocardial fibers have been reported as histopathological changes in the heart [ 25 , 30 , 43 ]. Recently, in veterinary medicine, LVH and DD have been reported in dogs with CS and those receiving high-dose prednisolone [ 9 , 22 , 44 ]. To our knowledge, current experimental studies on dogs are short-term evaluations [ 22 , 32 , 45 ] that report no obvious histopathological myocardial changes [ 22 ].…”

mentioning

confidence: 99%

“…Recently, in veterinary medicine, LVH and DD have been reported in dogs with CS and those receiving high-dose prednisolone [ 9 , 22 , 44 ]. To our knowledge, current experimental studies on dogs are short-term evaluations [ 22 , 32 , 45 ] that report no obvious histopathological myocardial changes [ 22 ]. In other words, the etiology of hyperglucocorticoidemia (HGC) causing LVH and DD in dogs remains elusive.…”

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confidence: 99%

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Long-term administration of prednisolone: Effects on the myocardial tissue of healthy beagle dogs

et al. 2021

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This study aimed to assess the structural and functional effects of long-term hyperglucocorticoidemia on canine myocardium and compare these parameters with histopathological changes. Twelve healthy male beagle dogs were enrolled and assigned to the high-dose prednisolone (P; n = 6) and control (C; n = 6) groups. The P group was treated with 2 mg/kg of prednisolone BID for 84 days. Clinical parameters were measured using echocardiography and non-invasive systolic blood pressure (SBP) measured before the initiation of synthetic corticosteroids and at 7, 28, 56, and 84 days after the start of medication. For histological evaluation, cardiovascular tissue was harvested from dogs in groups P (at the end of the medication period) and C (scheduled to be euthanized for unrelated reasons). In the P group, clinical changes including thickening of the left ventricular free wall (LVFW) and interventricular septum (IVS), decreased left ventricular (LV) diastolic function, and increased SBP were observed after the start of medication. During histological evaluation, fibrosis was observed in the LVFW and IVS in the P group. Furthermore, decreased glucocorticoid receptor (GCR) levels were observed in the LVFW, right ventricular free wall (RVFW), and IVS and increased mineralocorticoid receptor (MCR) levels were observed in the LVFW and RVFW in the P group compared with those in the C group. In conclusion, fibrosis may cause LV structural and functional abnormalities in dogs with hyperadrenocorticism. Furthermore, GCR downregulation and upregulated MCR might influence the myocardial fibrosis.

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confidence: 90%

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confidence: 99%

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confidence: 99%

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Long-term administration of prednisolone: Effects on the myocardial tissue of healthy beagle dogs

et al. 2021

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“…The model used for this study is that for cardiovascular research. A previous report examined a model in which dogs were administered a high dose synthetic corticosteroid at 2 mg/kg every 12 h for 28 days, which showed changes in echocardiographic cardiac morphology and function; however, histological changes were not observed [18]. Past studies have suggested that chronic hypercortisolism can affect cardiac function and morphology [19,20].…”

Section: Animalsmentioning

confidence: 99%

Effects of long-term and high-dose administration of glucocorticoids on the cranial cruciate ligament in healthy beagle dogs

Shimada

Wada²,

Tanaka³

et al. 2022

PLoS ONE

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This study aimed to determine the effects of long-term and high-dose administration of glucocorticoids (GCs) on the histological and mechanical properties of the cranial cruciate ligament (CrCL) in healthy beagle dogs. A synthetic corticosteroid at 2 mg/kg every 12 h was administered for 84 days in nine dogs (18 CrCLs) (GC group). Twenty CrCLs from 12 healthy male beagles were used as the normal control (control group). CrCLs were histologically examined (n = 12 in the GC group and n = 14 in the control group) using hematoxylin-eosin, Alcian-Blue, Elastica-Eosin stains, and immunohistological staining of type 1 collagen and elastin. An additional 12 CrCLs were mechanically tested (n = 6 in the GC and n = 6 in the control groups) to determine failure pattern, maximum tensile strength, maximum stress, elastic modulus, and stress and strain at the transition point. The histological examination revealed a significant increase in interfascicular area and fibrillar disorientation at the tibial attachment in both groups. The ratios of mucopolysaccharide-positive area and positive areas of elastic fibers were significantly higher in the control group than in the GC group. The biomechanical examination demonstrated significantly lower stress at the transition point in the GC group than in the control group. The present study results indicate that high-dose corticosteroids may affect metabolism, such as mucopolysaccharides and elastic fibers production, although the effect on type 1 collagen production is small. These changes of the extracellular matrix had a small effect on the strength of the ligament. This study suggested that the ligamentous changes associated with GC are different from the degeneration observed in spontaneous canine CrCL disease.

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“…Specifically, hypertrophy of cardiomyocytes and an increase in the cardiac fiber count were reported as histopathological changes ( Sugihara et al , 1992 ). Recently, left ventricular hypertrophy and cardiac dysfunction had been reported in dogs with HAC and in those experimentally treated with high-dose prednisolone ( Chen et al , 2014 ; Oui et al , 2015 ; Takano et al , 2015 ; Tanaka et al , 2021 ). A histopathological study of dogs showed no apparent change in the myocardium when a 2 mg/kg prednisolone dose was administered every 12 hours for 28 days ( Oui et al , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Long-term histological effects of high-dose prednisolone administration on the mitral valve in normal Beagle dogs

Tanaka¹,

Suzuki²,

Shimura³

et al. 2023

Open Vet J

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Background: In recent years, left ventricular hypertrophy and cardiac dysfunction have been reported in human and canine patients with hypercortisolism (HAC) and in dogs treated experimentally with high-dose prednisolone. However, to our knowledge, there have been no reports on the effects of hyperglucocorticism (HGC) on the mitral valve (MV). Aim: This study aimed to compare the MV in dogs treated with high-dose prednisolone with that in healthy dogs to investigate the effects of HGC on the MV. Methods: We investigated the effects of HGC on the MV by comparing samples obtained from high-dose glucocorticoid-treated (P) and healthy (C) dogs. The P group included healthy Beagle dogs (n = 6) treated with prednisolone (2 mg/kg, bid, po) for 84 days and the C group included healthy Beagle dogs (n = 6) euthanized for unrelated reasons. The anterior and posterior mitral leaflets (AML and PML, respectively) from both groups were harvested and stained with hematoxylin–eosin, Alcian blue, and Masson trichome. Additionally, adiponectin (ADN) and glucocorticoid receptor immunohistochemistry were performed. Histological evaluation was performed in the atrialis, spongiosa, fibrosa, and all layers of the proximal, middle, and distal regions of the AML and PML. Results: The proportion of the spongiosa layer thickness to the total thickness was higher in the P than in the C group (proximal and middle AML). However, the proportion of the fibrosa layer thickness to the total thickness was lower in the P than in the C group (middle PML). Areas of acidic sulfated mucosubstance deposition were smaller in the fibrosa layer and all layers (middle AML), while those of collagen deposition were smaller in the spongiosa and total layers (proximal and middle AML), in the P than in the C group. Additionally, ADN expression in the spongiosa layer was higher in the P than in the C group (middle AML). Conclusion: These findings suggest that long-term administration of synthetic glucocorticoids induces histological changes in the MV. These changes may lead to MV dysfunction in dogs with HGC.

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Tissue Doppler and strain imaging of left ventricle in Beagle dogs with iatrogenic hypercortisolism

Cited by 4 publications

References 26 publications

Long-term administration of prednisolone: Effects on the myocardial tissue of healthy beagle dogs

Long-term administration of prednisolone: Effects on the myocardial tissue of healthy beagle dogs

Effects of long-term and high-dose administration of glucocorticoids on the cranial cruciate ligament in healthy beagle dogs

Long-term histological effects of high-dose prednisolone administration on the mitral valve in normal Beagle dogs

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