Tissue distribution of transcription for 29 lipid metabolism-related genes in Takifugu rubripes, a marine teleost storing lipid predominantly in liver

Xu, Houguo; Meng, Xiaoxue; Liu, Jia; Wei, Yuliang; Sun, Bo; Liang, Mengqing

doi:10.1007/s10695-020-00815-7

Cited by 10 publications

(7 citation statements)

References 84 publications

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“…They store lipid predominantly in the liver, have a very low lipid content in the muscle, and do not have intraperitoneal adipose tissue [17,42]. The lipid metabolism in this fish species has evolved to adapt this special lipid storage pattern [43]. The lipid transport mechanisms, which were directly related to the fabp expression, could be special too in tiger puffer, but remain elusive to date and warrant further research.…”

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confidence: 99%

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

Wang

Zhao

Liao

et al. 2022

Aquaculture Nutrition

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Fatty acid-binding proteins (fabps) play important roles in lipid homeostasis. In the present study, 7 fabp isoforms, namely, fabp1, fabp2, fabp3, fabp4, fabp6, fabp7, and fabp10, in two marine teleosts, were characterized. In general, turbot and tiger puffer fabp genes showed high identity to their orthologs in other fish species and mammals, but tiger puffer Fabp6 shared the lowest identity to its known orthologs in zebrafish and human. The tissue distribution patterns of fabps were generally in accordance with their function features. However, tiger puffer fabps, in particular Fabp1, Fabp2, Fabp6 and Fabp7, may have functions distinct from other teleosts, as indicated by the phylogenetic tree and tissue distribution patterns. In both species, high dietary lipid levels downregulated the expression of fabp2, fabp3, fabp6, and fabp7a but tended to upregulate the fabp1 expression. Starvation downregulated the expression of most fabps in both fish species, but the downregulation of fabp expression in turbot was much more drastic and earlier compared to tiger puffer. Long-term (30-day) starvation increased the fabp7 expression in tiger puffer and tended to increase the fabp6 expression in turbot. Results of this study contribute to fish fabp physiology and its nutritional regulation.

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confidence: 99%

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

Wang

Zhao

Liao

et al. 2022

Aquaculture Nutrition

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“…Through fatty acid metabolism, the content of long-chain unsaturated fatty acids in phospholipid of cell membrane increased and the cell membrane would maintain stability and fluidity. In the current study, six genes was identified, which are involved in fatty acid metabolism pathway, these genes were acox1 (acyl-CoA oxidase 1, palmitoyl), acox3 (acyl-CoA oxidase 3, pristanoyl), acaa2 (acetyl-CoA acyltransferase 2), elovl4 (elongation of very long chain fatty acids elongase gene 4), acaca (acetyl-CoA carboxylases alpha) and fasn (fatty acid synthase) ( Xu et al, 2020 ; Yang et al, 2020 ; Wang et al, 2022d ; Liu et al, 2022 ). From the differential genes obtained in this study, the six genes were searched and the differences among tissues were analyzed.…”

Section: Methodsmentioning

confidence: 90%

“…Fatty acid metabolism was important for fish to survive at low temperatures, such as Larimichthy crocea , Cyprinus carpio , Chanos , Ctenopharyngodon Idella ( Farkas et al, 1980 ; Hsieh et al, 2003 ; Hsieh and Kuo, 2005 ; Qian and Xue, 2016 ). Tissue distribution of transcription for 29 lipid metabolism-related genes in Takifugu rubripes demonstrated that in fatty acid β-oxidation, acox1 and acox3 gene expression levels were higher in liver than in other tissues ( Xu et al, 2020 ). The expression levels of acaa2 gene, related to fatty acid metabolism, would change when affected by certain environment ( Yang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis and gene expression analysis related to salinity-alkalinity and low temperature adaptation of Triplophysa yarkandensis

Zhang

Wang²,

Zhai

et al. 2023

Front. Genet.

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T. yarkandensis is a common species of Triplophysa, and it is distributed in Shule river of Hexi Corridor, of Gansu province in China. In order to enrich gene database resources and explore the environment adaptation of T. yarkandensis, fifteen tissues were collected from three adult T. yarkandensis for transcriptome sequencing and de novo assembly. Nine major international gene annotation databases (NR, COG, egg_NOG, TrEMBL, Pfam, KOG, Swiss prot, KEGG and Gene Ontology) were utilized to annotate unigenes. A detailed study was conducted to explore the gene expression and the differentially expressed genes among five tissues (brain, heart, kidney, liver and spleen). In addition, the current study showed that candidate genes involved in salinity-alkalinity and low temperature adaptation were differentially expressed in tissues of T. yarkandensis. Precisely, mapk1, abcc1, gpx1, gpx4, cat and aqp1 genes participated in the regulation process of salinity-alkalinity adaptation, and elovl4, acaca, fasn, acaa2, acox1 and acox3 genes were involved in fatty acid metabolism and closely associated with low temperature adaptation. On the one hand, it was found that the expression of these genes varied among different tissues, and the important pathways involved in these genes were mapped. Furthermore, we analyzed mapk1 and acox1 genes in depth to obtain the predicted gene structure and important amino acid sites. The transcriptome information in this study will be conducive to provide further understanding for the molecular level research and exploration of the environmental adaptation of T. yarkandensis.

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“…However, for tiger puffer, besides lipid metabolism, the liver also functions as lipid storage organ, which may weaken the function of cholesterol metabolism. Our previous studies have suggested that in tiger puffer, the intestine is probably a lipid metabolism center, but the liver may function as a pure lipid storage organ [ 49 ]. Another explanation of this difference could be the fact that tiger puffer body composition has a higher buffer capacity than turbot in response to dietary regulation, which has been indicated by our previous studies [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary Cholesterol Supplementation Inhibits the Steroid Biosynthesis but Does Not Affect the Cholesterol Transport in Two Marine Teleosts: A Hepatic Transcriptome Study

Zi-ling

Xiong

Meng

et al. 2023

Aquaculture Nutrition

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Cholesterol has been used as additive in fish feeds due to the reduced use of fish meal and fish oil. In order to evaluate the effects of dietary cholesterol supplementation (D-CHO-S) on fish physiology, a liver transcriptome analysis was performed following a feeding experiment on turbot and tiger puffer with different levels of dietary cholesterol. The control diet contained 30% fish meal (0% fish oil) without cholesterol supplementation, while the treatment diet was supplemented with 1.0% cholesterol (CHO-1.0). A total of 722 and 581 differentially expressed genes (DEG) between the dietary groups were observed in turbot and tiger puffer, respectively. These DEG were primarily enriched in signaling pathways related to steroid synthesis and lipid metabolism. In general, D-CHO-S downregulated the steroid synthesis in both turbot and tiger puffer. Msmo1, lss, dhcr24, and nsdhl might play key roles in the steroid synthesis in these two fish species. Gene expressions related to cholesterol transport (npc1l1, abca1, abcg1, abcg2, abcg5, abcg8, abcb11a, and abcb11b) in the liver and intestine were also extensively investigated by qRT-PCR. However, the results suggest that D-CHO-S rarely affected the cholesterol transport in both species. The protein-protein interaction (PPI) network constructed on steroid biosynthesis-related DEG showed that in turbot, Msmo1, Lss, Nsdhl, Ebp, Hsd17b7, Fdft1, and Dhcr7 had high intermediary centrality in the dietary regulation of steroid synthesis. In conclusion, in both turbot and tiger puffer, the supplementation of dietary cholesterol inhibits the steroid metabolism but does not affect the cholesterol transport.

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Tissue distribution of transcription for 29 lipid metabolism-related genes in Takifugu rubripes, a marine teleost storing lipid predominantly in liver

Cited by 10 publications

References 84 publications

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

Transcriptome analysis and gene expression analysis related to salinity-alkalinity and low temperature adaptation of Triplophysa yarkandensis

Dietary Cholesterol Supplementation Inhibits the Steroid Biosynthesis but Does Not Affect the Cholesterol Transport in Two Marine Teleosts: A Hepatic Transcriptome Study

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