Tissue Distribution of the Human Soluble Guanylate Cyclases

Budworth, Joanna; Meillerais, Sylvie; Charles, Ian G.; Powell, K.

doi:10.1006/bbrc.1999.1444

Cited by 116 publications

(93 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the endothelial progenitor marker, FLK1, was signifi cantly down-regulated by 34% or 33%, respectively, after treatment with l -NAME (10 −5 M with sGCα 3 and sGCβ 3 at day 14 of differentiation. However, sGCα 3 and sGCβ 3 were also expressed in other cell types besides ESC-ECs, which is consistent with literature indicating that sGCs are expressed in numerous mammalian tissues [ 23 ]. These results indicated that NO-signaling components are present in ESCs, but are up-regulated during the course of ESC differentiation.…”

Section: Effect Of L -Name On Endothelial Differentiation Of Escssupporting

confidence: 91%

Role of Nitric Oxide Signaling in Endothelial Differentiation of Embryonic Stem Cells

Huang

Fleißner

Sun

et al. 2010

Stem Cells and Development

View full text Add to dashboard Cite

Signaling pathways that govern embryonic stem cell (ESCs) differentiation are not well characterized. Nitric oxide (NO) is a potent vasodilator that modulates other signaling pathways in part by activating soluble guanylyl cyclase (sGC) to produce cyclic guanosine monophosphate (cGMP). Because of its importance in endothelial cell (EC) growth in the adult, we hypothesized that NO may play a critical role in EC development. Accordingly, we assessed the role of NO in ESC differentiation into ECs. Murine ESCs differentiated in the presence of NO synthase (NOS) inhibitor NG-nitroarginine methyl ester ( l -NAME) for up to 11 days were not signifi cantly different from vehicle-treated cells in EC markers. However, by 14 days, l -NAME-treated cells manifested modest reduction in EC markers CD144, FLK1, and endothelial NOS. ESC-derived ECs generated in the presence of l -NAME exhibited reduced tube-like formation in Matrigel. To understand the discrepancy between early and late effects of l -NAME, we assessed the NOS machinery and observed low mRNA expression of NOS and sGC subunits in ESCs, compared to differentiating cells after 14 days. In response to NO donors or activation of NOS or sGC, cellular cGMP levels were undetectable in undifferentiated ESCs, at low levels on day 7, and robustly increased in day 14 cells. Production of cGMP upon NOS activation at day 14 was inhibited by l -NAME, confi rming endogenous NO dependence. Our data suggest that NOS elements are present in ESCs but inactive until later stages of differentiation, during which period NOS inhibition reduces expression of EC markers and impairs angiogenic function.

show abstract

Section: Effect Of L -Name On Endothelial Differentiation Of Escssupporting

confidence: 91%

Role of Nitric Oxide Signaling in Endothelial Differentiation of Embryonic Stem Cells

Huang

Fleißner

Sun

et al. 2010

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…Differential expression of mRNAs for a and b-sGC also influences the formation of functional heterodimers. For example, in pituitary cells, the mRNA levels (Budworth et al 1999) and the protein levels (Kostic et al 2004) for a1-sGC are lower than b1-sGC. Consistent with these findings, the overexpression of a1-sGC alone in immortalized pituitary cells is sufficient to make several fold increase in NO-stimulated cGMP production (Kostic et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of α1-soluble guanylyl cyclase gene promoter in rat pituitary cells

Jiang

Stojilković

2006

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Soluble guanylyl cyclase is a cytosolic enzyme which catalyzes conversion of GTP to the second messenger cyclic GMP. The transcriptional regulation at the promoter levels of four soluble guanylyl cyclase subunits, termed a1, a2, b1, and b2, is largely unknown. In this study, we identified the transcription start site of a1-soluble guanylyl cyclase gene in rat pituitary cells and cloned the 3 . 5 kb 5 0 -promoter. Sequence analysis of this TATA-less promoter revealed the presence of several putative-binding sites for transcriptional factors, including CCAAT site at K41 to K32 and Sp1 site at K34 to K24. Transfection of pituitary cells with constructs of variable lengths confirmed the relevance of different promoter regions in the control of transcriptional activity. Among them, the K49 to C156 region was critical for basal transcriptional activity. Electrophoretic mobility shift assay using nuclear proteins extracted from normal and immortalized pituitary cells indicated that the CCAAT/Sp1 site within the K49 to C156 region was able to specifically interact with CCAAT-binding factor and Sp1. These two sites were partly overlapped and both of them conferred stimulatory effects. The in vivo recruitment of CCAAT-binding factor and Sp1 was confirmed by chromatin immunoprecipitation. These results indicate that the composite CCAAT/Sp1 cis-element contributes to the expression of a1-sGC subunit in resting pituitary cells.

show abstract

“…Soluble GCa1 and R1 isoforms are expressed almost ubiquitously (Table 2) [84][85][86][87][88][89][90]. A1pha2 isoform expression is detected in various but more limited tissues than al and ~1 isoforms ( The chromosomal localization of the human genes has been determined for all of the GCs.…”

Section: -19] Gc-a or Natriuretic Peptide Receptor-a (Npr-a) Is Thmentioning

confidence: 99%

The Regulation and Physiological Roles of the Guanylyl Cyclase Receptors.

2001

View full text Add to dashboard Cite

Tissue Distribution of the Human Soluble Guanylate Cyclases

Cited by 116 publications

References 29 publications

Role of Nitric Oxide Signaling in Endothelial Differentiation of Embryonic Stem Cells

Role of Nitric Oxide Signaling in Endothelial Differentiation of Embryonic Stem Cells

Molecular cloning and characterization of α1-soluble guanylyl cyclase gene promoter in rat pituitary cells

The Regulation and Physiological Roles of the Guanylyl Cyclase Receptors.

Contact Info

Product

Resources

About