Tissue distribution and toxicity effects of myclobutanil enantiomers in lizards (Eremias argus)

Chen, Li; Li, Ruiting; Diao, Jinling; Tian, Zhongnan; Di, Shanshan; Zhang, Wenjun; Cheng, Cheng; Zhou, Zhiqiang

doi:10.1016/j.ecoenv.2017.07.017

Cited by 14 publications

(4 citation statements)

References 36 publications

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“…The EF values were less than 0.5 with time in the liver, kidney, heart, lung and testis, indicating enantioselective enrichment of (−)-MT in these tissues. Similarly, preferential enrichment of (−)-MT was also found in the kidney, heart and lung of lizards exposed to 20 mg·kg –1 bw rac -MT . In contrast, a study reported that there was an enrichment of (+)-MT in Scenedesmus obliquus .…”

Section: Resultsmentioning

confidence: 93%

“…Similarly, preferential enrichment of (−)-MT was also found in the kidney, heart and lung of lizards exposed to 20 mg•kg −1 bw rac-MT. 33 In contrast, a study reported that there was an enrichment of (+)-MT in Scenedesmus obliquus. 34 These data demonstrated that enantioselective enrichment of MT in nontarget organisms is species-specific.…”

Section: ■ Materials and Methodsmentioning

confidence: 93%

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Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats

Wang

Zhu

et al. 2018

Environ. Sci. Technol.

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Myclobutanil (MT), a chiral fungicide, can be metabolized enantioselectively in organisms. In this work, the associated absorption, distribution, metabolism and transcriptional responses of MT in rats were determined following a single-dose (10 mg·kg body weight) exposure to rac-, (+)- or (-)-MT. The enantiomer fractions (EFs) were less than 0.5 with time in the liver, kidney, heart, lung, and testis, suggesting preferential enrichment of (-)-MT in these tissues. Furthermore, there was conversion of (+)-form to (-)-form in the liver and kidney after 6 h exposure to enantiopure (+)-MT. Enrichment and degradation of the two enantiomers differed between rac-MT and MT-enantiomers groups, suggesting that MT bioaccumulation is enantiomer-specific. Interestingly, the degradation half-life of MT in the liver with rac-MT treatment was shorter than that with both MT-enantiomer treatments. One reason may be that the gene expression levels of cytochrome P450 1a2 ( cyp1a2) and cyp3a2 genes in livers treated with rac-MT were the highest among the three exposure groups. In addition, a positive correlation between the expression of cyp2e1 and cyp3a2 genes and rac-MT concentration was found in livers exposed to rac-MT. Simultaneously, five chiral metabolites were detected, and the enantiomers of three metabolites, RH-9090, RH-9089, and M2, were separated. The detected enantiomers of (+)-MT metabolites were in complete contrast with those of (-)-MT metabolites. According to the results, a metabolic pathway of MT in male rats was proposed, which included the following five metabolites: RH-9089, RH-9090, RH-9090 Sulfate, M1, and M2. The possible metabolic enzymes were marked in the pathway. The findings of this study provide more specific insights into the enantioselective metabolic mechanism of chiral triazole fungicides.

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Section: Resultsmentioning

confidence: 93%

Section: ■ Materials and Methodsmentioning

confidence: 93%

Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats

Wang

Zhu

et al. 2018

Environ. Sci. Technol.

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“…Based on the results we determine whether the model was successful. Organ coefficient is a common indicator of chronic toxicity [ 35 , 36 ]. We clarified that Asarum can lead to increased organ coefficients.…”

Section: Discussionmentioning

confidence: 99%

New Contributions to Asarum Powder on Immunology Related Toxicity Effects in Lung

Han

Huang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Objective. Asarum is widely used in clinical practice of Chinese medicine in the treatment of respiratory diseases. Many toxic ingredients (safrole, etc.) had been found in Asarum that show multiple visceral toxicities. In this study, we performed systematic investigation of expression profiles of genes to take a new insight into unclear mechanism of Asarum toxicities in lung. Methods. mRNAs were extracted from lungs of rats after intragastric administration with/without Asarum powders, and microarray assays were applied to investigate gene expression profiles. Differentially expressed genes with significance were selected to carry out GO analysis. Subsequently, quantitative PCRs were performed to verify the differential expression of Tmprss6, Prkag3, Nptx2, Antxr11, Klk11, Rag2, Olr77, Cd7, Il20, LOC69, C6, Ccl20, LOC68, and Cd163 in lung. Changes of Ampk, Bcl2, Caspase 3, Il1, Il20, Matriptase2, Nfκb, Nptx2, and Rag2 in the lung on protein level were verified by western blotting and immunohistochemistry. Results. Compared with control group, the estimated organ coefficients were relatively increased in Asarum group. Results of GO analysis showed that a group of immune related genes in lung were expressed abnormally. The result of PCRs showed that Ccl20 was downregulated rather than other upregulated genes in the Asarum group. Western blotting and immunohistochemistry images showed that Asarum can upregulate the expression of Ampk, Caspase 3, Il1, Il20, Matriptase2, Nfκb, and Rag2 and downregulate the expression of Bcl2 in lung. Conclusion. Our data suggest that expressions of immune related genes in lung were selectively altered by Asarum. Therefore, inflammatory response was active, by regulating Caspase 3, Il1, Il20, Matriptase2, Nfκb, Rag2, Tmprss6, Prkag3, Nptx2, Antxr1, Klk11, Olr77, Cd7, LOC69, C6, LOC68, Cd163, Ampk, Bcl2, and Ccl20. Our study indicated that inflammatory factors take effect in lung toxicity caused by Asarum, which provides a new insight into molecular mechanism of Asarum toxicities in lung.

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“… 5,6 On the basis of environmental assessments, the exposure risk of triazole fungicides tends to be greater for freshwater fish, invertebrates, birds, and mammals, depending on the application pattern and rate. 7 They exert estrogenic effects by interfering with estrogen receptors in mammals. 8 As a probable human carcinogen, it also induces apoptosis of human trophoblast cells.…”

Section: Introductionmentioning

confidence: 99%

Air-assisted ionic liquid dispersive liquid–liquid microextraction based on solidification of the aqueous phase for the determination of triazole fungicides in water samples by high-performance liquid chromatography

et al. 2019

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Tissue distribution and toxicity effects of myclobutanil enantiomers in lizards (Eremias argus)

Cited by 14 publications

References 36 publications

Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats

Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats

New Contributions to Asarum Powder on Immunology Related Toxicity Effects in Lung

Air-assisted ionic liquid dispersive liquid–liquid microextraction based on solidification of the aqueous phase for the determination of triazole fungicides in water samples by high-performance liquid chromatography

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