Tissue‐dependent upregulation of rat uncoupling protein‐2 expression in response to fasting or cold

Boss, Olivier; Samec, Sonia; Dulloo, Abdul G.; Seydoux, Josiane; Muzzin, Patrick; Giacobino, Jean‐Paul

doi:10.1016/s0014-5793(97)00755-2

Cited by 215 publications

(183 citation statements)

References 12 publications

(18 reference statements)

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“…Another candidate seems free fatty acid. UCP2 and UCP3 expression in skeletal muscle have been reported to increase in response to fasting [1,8] , which is a representative cue to elevate plasma free fatty acids. The present results showed that administration of CL produced a sustained elevation of plasma free fatty acids as a result of their potent lipomobilizing action on adipose tissues [19,24].…”

Section: Discussionmentioning

confidence: 99%

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

Nakamura

Nagase

Asano

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Chronic stimulation of the β3-adrenergic receptor (AR) in obese animals resulted in a reduced adiposity associated with an increased expression of thermogenic uncoupling protein (UCP)1 in adipose tissues. In this study, the mRNA expression of newly cloned UCP isoforms (UCP2 and UCP3) were examined in obese yellow KK and C57BL control mice. UCP2 mRNA was found in all tissues examined, with higher levels in adipose tissues and skeletal muscle of the obese mice. UCP3 mRNA was expressed in skeletal muscle, heart and brown adipose tissue similarly in the two mouse strains. Daily injection of a selective β3-adrenergic agonist, CL316,243 (0.1 mg/kg), for 10 days resulted in a marked reduction of white fat pad weight and 1.8~4.8-fold increase in the mRNA levels of UCP2 and UCP3 in skeletal muscle of obese mice. No noticeable change in the UCP2 and 3 mRNA levels was found in brown and white adipose tissues. It was also found that CL316,243 injection produced a marked and sustained elevation of the plasma free fatty acid level. These results, together with our previous findings of the fatty acid-induced UCP expression in a myocyte cell line in vitro, suggest that the β3-AR agonist-induced UCP expression in skeletal muscle may be mediated through the elevated plasma free fatty acids. It was also suggested that anti-obesity effect of β3-AR agonists is attributable to increased thermogenesis not only by UCP1 but also by UCP2 and UCP3.KEY WORDS: adipose tissue, β3-adrenergic agonist, fatty acid, obese mouse, uncoupling protein.

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Section: Discussionmentioning

confidence: 99%

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

Nakamura

Nagase

Asano

et al. 2001

The Journal of Veterinary Medical Science

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“…In vivo studies indicate that physiological and pathological elevation of blood long-chain fatty acids (up to 2-to 3-fold) resulting from fasting (16,(47)(48)(49)(50), high fat diet (12), suckling of newborn pups (51), sepsis (52), or streptozotocin-induced diabetes (53, 54) induce up-regulation of Ucp2. Similarly, in vitro studies have shown that monounsaturated (n-9) and polyunsaturated (n-6 and n-3) fatty acids can dramatically induce up-regulation of Ucp2 in 3T3-L1 preadipocytes (55), human primary cultured myotubes (56), the rat myoblast cell line L6 (57), the rat insulinoma cell line INS-1 (58, 59), rat primary cultured adipocytes (60), and cloned bovine mammary epithelial cells (61).…”

Section: Regulation By Long-chain Fatty Acidsmentioning

confidence: 99%

Nutritional and hormonal regulation of uncoupling protein 2

et al. 2009

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SummaryUncoupling proteins (UCPs) belong to a family of mitochondrial carrier proteins that are present in the mitochondrial inner membrane. Genetic and experimental studies have shown that UCP dysfunction can be involved in metabolic disorders and in obesity. Uncoupling protein-1 (UCP1; also known as thermogenin) was identified in 1988 and found to be highly expressed in brown adipose tissue. UCP1 allows the leak of protons in respiring mitochondria, dissipating the energy as heat; the enzyme has an important role in nonshivering heat production induced by cold exposure or food intake. In 1997, two homologs of UCP1 were identified and named UCP2 and UCP3. These novel proteins also lower mitochondrial membrane potential, but whether they can dissipate metabolic energy as heat as efficiently as UCP1 is open to dispute. Even after a decade of study, the physiological roles of these novel proteins have still not been completely elucidated. This review aims to shed light on the nutritional and hormonal regulation of UCP2 and on its physiological roles.

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“…Animal models suggest that many tissues, including brown adipose tissue (Boss et al 1997;Denjean et al 1999;Yamashita et al 1999), white adipose tissue (Yamashita et al 1999), heart (Boss et al 1997), soleus muscle (Boss et al 1997), spinal cord (Mizuno et al 2000), and spleen (Ganta et al 2006) do indeed display at least some changes in gene expression in response to cold. Hypothermia can also modify gene expression responses to ischemia/reperfusion of brain (Fukui et al 2006;Kobayashi et al 2008;Ohta et al 2007;Yenari and Han 2006) and liver (Niemann et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In the spleen, changes in cytokine and chemokine expression occur even after sympathetic denervation, suggesting that cold itself, and not the increase in sympathetic activity that occurs during hypothermic exposure, may be responsible for some of the changes seen (Ganta et al 2006). The importance of examining different tissues is illustrated by the observation that in rat (Boss et al 1997;Mizuno et al 2000), UCP-2 responses to hypothermia appear to vary somewhat as a function of tissue type.…”

Section: Introductionmentioning

confidence: 99%

A microarray analysis of the effects of moderate hypothermia and rewarming on gene expression by human hepatocytes (HepG2)

Sonna

Kuhlmeier

Khatri

et al. 2010

Cell Stress and Chaperones

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The gene expression changes produced by moderate hypothermia are not fully known, but appear to differ in important ways from those produced by heat shock. We examined the gene expression changes produced by moderate hypothermia and tested the hypothesis that rewarming after hypothermia approximates a heat-shock response. Six sets of human HepG2 hepatocytes were subjected to moderate hypothermia (31°C for 16 h), a conventional in vitro heat shock (43°C for 30 min) or control conditions (37°C), then harvested immediately or allowed to recover for 3 h at 37°C. Expression analysis was performed with Affymetrix U133A gene chips, using analysis of variance-based techniques. Moderate hypothermia led to distinct time-dependent expression changes, as did heat shock. Hypothermia initially caused statistically significant, greater than or equal to twofold changes in expression (relative to controls) of 409 sequences (143 increased and 266 decreased), whereas heat shock affected 71 (35 increased and 36 decreased). After 3 h of recovery, 192 sequences (83 increased, 109 decreased) were affected by hypothermia and 231 (146 increased, 85 decreased) by heat shock. Expression of many heat shock proteins was decreased by hypothermia but significantly increased after rewarming. A comparison of sequences affected by thermal stress without regard to the magnitude of change revealed that the overlap between heat and cold stress was greater after 3 h of recovery than immediately following thermal stress. Thus, while some overlap occurs (particularly after rewarming), moderate hypothermia produces extensive, timedependent gene expression changes in HepG2 cells that differ in important ways from those induced by heat shock.

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Tissue‐dependent upregulation of rat uncoupling protein‐2 expression in response to fasting or cold

Cited by 215 publications

References 12 publications

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

Nutritional and hormonal regulation of uncoupling protein 2

A microarray analysis of the effects of moderate hypothermia and rewarming on gene expression by human hepatocytes (HepG2)

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