Tissue Culture Studies on Human Skin

Hu, Funan

doi:10.1001/archderm.1954.01540190003001

Cited by 10 publications

(2 citation statements)

References 13 publications

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“…Germ tubes formed by either C. albicans or C. stellatoidea on TOC medium are identical in morphology, making differentiation between the two yeasts on this basis impossible. It should also be emphasized that the germ tube morphology on TOC medium is compatible with its classical description in biological fluids (3,13), since the tube lacks any constriction at its point of origin (Fig. 1).…”

Section: Resultssupporting

confidence: 65%

New culture medium for the presumptive identificaion of Candida albicans and Cryptococcus neoformans

1977

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A new medium composed of Tween 80, oxgall, caffeic acid, and Davis agar (TOC) that provides for the rapid presumptive identification of Candida albicans and Cryptococcus neoformans is described herein. C. albicans is differentiated from other yeasts by the sequential production of germ tubes and chlamydospores. In a comparison with cormeal agar control plates, there was an increase of chlamydospore-forming strains of C. albicans (97.1% versus 87.2%) and a decrease in the time required for chlamydospore formation (24 h versus 48 h). C. neoformans produced a brown pigment of TOC, which is specific for its identification, thus differentiating it from the other yeasts. A comparison of 24-h pigment production by C. neoformans on TOC with that of birdseed agar showed a dark, coffee brown color in the former cultures and a light brown color in the latter. The change in pigmentation of C. neoformans, as well as morphological changes in C. albicans, can be induced within 3 to 12 h and in not more than 24 h on the TOC medium.

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Section: Resultssupporting

confidence: 65%

New culture medium for the presumptive identificaion of Candida albicans and Cryptococcus neoformans

1977

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“…The blastospores of Candida albicans germinate when incubated at 37 C in serum or plasma from humans and other animals (6,8,19), and this fact has been developed into a useful diagnostic test for identification of yeast isolates from clinical materials (3,5,15,20,23). Landau et al (12) established that tissue culture medium 199 (TC 199) supports germ tube production by C. albicans nearly as well as does serum.…”

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confidence: 99%

Germination of Candida albicans induced by proline

Dabrowa¹,

Taxer²,

Howard³

1976

Infect Immun

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Blastospores of Candida albicans germinated in a proline-biotin-buffer medium incubated at 37 C. Certain other amino acids in the glutamate, aspartate, and pyruvate families also fostered germination but generally to a lesser extent than did proline. L-Cysteine, D-proline, and certain structural analogues of Lproline inhibited proline-stimulated germination. The concentration of phosphate and glucose was crucial to amino acid-stimulated germination of C. albicans. Clinical isolates and stock cultures varied in their response to the germ tube-inducing activity of proline or other amino acids. The proline-buffer medium cannot be used in a diagnostic test for production of germ tubes by isolates of yeasts.

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Evaluation of SOC for the presumptive identification of Candida albicans and Cryptococcus neoformans

Fleming¹,

Knezek²,

Dorn³

1987

Mycopathologia

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SOC, a fungal growth medium composed of Solryth, oxgall, and caffeic acid, was evaluated as a medium to provide rapid, differential identification of Candida albicans and Cryptococcus neoformans. Using a variety of common isolation media to produce the yeast inocula, the germ tube methods tested ranked in the following order of decreasing sensitivity: SOC (97% +/- 1), serum (92% +/- 5), rabbit coagulase plasma with EDTA in combination with tryptic soy broth (89% +/- 5), TOC (89% +/- 6), and rabbit coagulase plasma with EDTA (83% +/- 4). In chlamydospore production, SOC also proved to be the most sensitive after 24 h incubation: SOC (96% +/- 2), TOC (80% +/- 2), and cornmeal-Tween 80 agar (14% +/- 3). Other medically important yeasts showed normal patterns of growth within 24 h on SOC, thus assisting in their identification. Eighty strains of Cryptococcus neoformans showed characteristic brown pigmentation on SOC and TOC within 18 h, while all other species of the genus Cryptococcus and 229 Candida isolates did not show a change in pigmentation.

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Tissue Culture Studies on Human Skin

Cited by 10 publications

References 13 publications

New culture medium for the presumptive identificaion of Candida albicans and Cryptococcus neoformans

New culture medium for the presumptive identificaion of Candida albicans and Cryptococcus neoformans

Germination of Candida albicans induced by proline

Evaluation of SOC for the presumptive identification of Candida albicans and Cryptococcus neoformans

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