Tissue Culture Studies on Bacterial Hypersensitivity

Moen, Johannes K.

doi:10.1084/jem.64.3.355

Cited by 32 publications

(7 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). As already reported by Moen [18] repeated skin testing of the same nonreactive animal frequently results in a positive skin reaction to subsequent testing. It seems, therefore, that the material employed for skin testing can lead to active sensitization.…”

Section: Cosupporting

confidence: 65%

Hypersensitivity Reactions in Guinea Pigs to Group A Hemolytic Streptococci

Dekaris¹,

Alouf²,

Raynaud³

1970

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Section: Cosupporting

confidence: 65%

Hypersensitivity Reactions in Guinea Pigs to Group A Hemolytic Streptococci

Dekaris¹,

Alouf²,

Raynaud³

1970

Int Arch Allergy Immunol

View full text Add to dashboard Cite

“…If we infer from this experience that a relatively high level of delayed hypersensitivity is necessary for the occurrence of the corneal response, the present results suggest that the wax treatment is very effective in this regard with egg albumin antigen. (8,(19)(20)(21)(22) point to the usefulness of tissue.…”

Section: Relationship Of the Delayed Cutaneous Response To The Cormentioning

confidence: 99%

The Rôle of the "Wax" of the Tubercle Bacillus in Establishing Delayed Hypersensitivity

Raffel

Arnaud

Dukes

et al. 1949

Journal of Experimental Medicine

View full text Add to dashboard Cite

Recent publications (I, 2) have described the influence of the "wax" of the human tubercle bacillus in causing guinea pigs to respond with the delayed form of hypersensitivity to tuberculoprotein. In all essential features the hypersensitive state established by the use of these two bacteria-free components of the bacillus reproduced the allergy of infection, including cutaneous and systemic responsiveness to old tuberculin, a modified (non-necrotizing) Koch reactivity to the intracutancous injection of bacilli, and reactivity of explanted tissue to the presence of tuberculin.A natural sequence to these observations has been the question of the influence of this lipoidal fraction in altering the type of hypersensitivity to other antigenic substances unrelated to the tubercle bacillus. Since tuberculoprotcin alone performs immunologically like any other antigenic protein, inducing humoral antibody and anaphylactic sensitivity, and since these activities are extended by the addition of the wax to include induction of delayed allergy, it is reasonable to suppose that this function of the wax might reveal itself as well with any antigen. This view has bccn implemented with evidence in the case of picryl chloride (3).Over twenty years ago Dicnes and his coworkers (4-6) showed that the injection of egg white into tuberculous guinea pigs, or into animals receiving injections of killcd tubercle bacilli, resulted in a type of reactivity to intracutaneous injection with this antigen which differed considerably from that ordinarily seen in animals sensitized with egg white. In the tuberculous animals the dermal reactions to egg white persisted for 48 hours, and were often necrotic. In contrast, skin tests in sensitized non-tuberculous animals resulted in the usual edematous reactions of the Arthus type which reached their peak within a few hours and vanished or waned considerably within 24 hours. The former reaction resembles the tuberculin type of hypersensitive response; the * This work has been

show abstract

“…that was toxic for normal cells, however, was more than six times as great as that required to elicit a similar degree of toxicity for sensitive cells. In experiments with extracts of group C hemolytic streptococci Moen found that a concentration of 1-6000 of the extract was toxic for sensitive cells, but only slightly toxic for normal cells (3). The amount of streptococcal antigen suitable for demonstrating dermal sensitivity in infected animals was 0.1 ml.…”

Section: Discussionmentioning

confidence: 98%

Tissue Culture Studies on Bacterial Allergy in Experimental Brucellosis

Heilman

Howard

Carpenter

1958

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Tissue culture methods have been used to investigate infectious allergy in experimental brucellosis. A study was made of the effect of whole cell Brucella antigen on cultures of spleen from normal and Brucella-infected guinea pigs. The degree of toxicity was based upon the inhibition of migration of wandering cells and upon the morphologic appearance of stained sections of tissue cultures at different periods of incubation. A suspension of heat-killed Br. suis was more toxic for splenic cells from guinea pigs infected with Br. suis than for normal splenic cells. Macrophages were more sensitive than leucocytes to the toxic action of the antigen. The degenerative changes observed in Brucella-sensitive cells exposed to the antigen were similar to the degeneration previously observed in cultures of tuberculin-sensitive cells in the presence of tuberculin. The specific toxicity of the whole Brucella antigen, however, was more marked than that of tuberculin. Preliminary experiments indicate that serum and plasma containing specific antibodies obtained from Brucella-infected guinea pigs reduce the toxic effect of the antigen in cultures of both normal and Brucella-sensitive cells. The protective action of the homologous antiserum was greater for Brucella-sensitive cells than for normal cells.

show abstract

Tissue Culture Studies on Bacterial Hypersensitivity

Cited by 32 publications

References 1 publication

Hypersensitivity Reactions in Guinea Pigs to Group A Hemolytic Streptococci

Hypersensitivity Reactions in Guinea Pigs to Group A Hemolytic Streptococci

The Rôle of the "Wax" of the Tubercle Bacillus in Establishing Delayed Hypersensitivity

Tissue Culture Studies on Bacterial Allergy in Experimental Brucellosis

Contact Info

Product

Resources

About