Tissue culture of wild-type, interspecific genome/plastome hybrids and plastome mutants of evening primrose ( Oenothera) : controlled morphogenesis and transformation

Mehra-Palta, Asha; Koop, Hans‐Ulrich; Goes, S.; Troidl, E.-M.; Nagy, Gábor; Tyagi, S.; Kofer, Waltraud; Herrmann, Reinhold G.

doi:10.1007/s002990050451

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…Technologies for ecient transformation and for cell and tissue culture of Oenothera have been developed (Kuchuk et al 1998;Mehra-Palta et al 1998). Technologies for ecient transformation and for cell and tissue culture of Oenothera have been developed (Kuchuk et al 1998;Mehra-Palta et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Complete nucleotide sequence of the Oenothera elata plastid chromosome, representing plastome I of the five distinguishable Euoenothera plastomes

Hupfer¹,

Świątek²,

Hornung³

et al. 2000

Mol Gen Genet

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We describe the 159,4443-bp sequence of the plastid chromosome of Oenothera elata (evening primrose). The Oe. elata plastid chromosome represents type I of the ®ve genetically distinguishable basic plastomes found in the subsection Euoenothera. The genus Oenothera provides an ideal system in which to address fundamental questions regarding the functional integration of the compartmentalised genetic system characteristic of the eukaryotic cell. Its highly developed taxonomy and genetics, together with a favourable combination of features in its genetic structure (interspeci®c fertility, stable heterozygous progeny, biparental transmission of organelles, and the phenomenon of complex heterozygosity), allow facile exchanges of nuclei, plastids and mitochondria, as well as individual chromosome pairs, between species. The resulting hybrids or cybrids are usually viable and fertile, but can display various forms of developmental disturbance.

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Section: Introductionmentioning

confidence: 99%

Complete nucleotide sequence of the Oenothera elata plastid chromosome, representing plastome I of the five distinguishable Euoenothera plastomes

Hupfer¹,

Świątek²,

Hornung³

et al. 2000

Mol Gen Genet

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“…(evening primrose) belonging to Onagraceae family were recorded (Skrzypczak et al 1994;Pavingerova et al 1996;Kuchuk et al 1998;Mehra-Palta et al 1998;Thiem et al 1999;De Gyves et al 2001). Three of the studies showed that BA was the most successful growth regulator for evening primrose (Skrzypczak et al 1994;Mehra-Palta et al 1998;Kuchuk et al 1998), like fireweed. Pavingerova et al (1996) reported isopentyladenine was the most effective for the regeneration of evening primrose.…”

Section: Resultsmentioning

confidence: 96%

Efficient in vitro regeneration of fireweed, a medicinal plant

2008

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Epilobium angustifolium L. (fireweed) is a medicinal plant that has been used to treat diarrhea, mucous colitis, irritable-bowel syndrome, skin problems, prostate problems, menstrual disorders, asthma, whooping cough, and hiccups. A highly efficient and rapid regeneration system via multiple shoot formation was developed for fireweed. Explants (leaf, petiole, root, and stem segments) excised from sterile seedlings were cultured on medium supplemented with different concentrations and combinations of various plant growth regulators. Explant browning, a major problem for regeneration, was overcome by adding 100 mg/l ascorbic acid to all prepared media containing growth regulator combinations. Root explants formed more shoots than other explants. Best shoot proliferation was obtained from root explants cultured on media with 0.

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“…are available (e.g. Mehra-Palta et al, 1998;Pavingerova, Galis & Ondrej, 1996), but these proved unsatisfactory when applied to the commercial cultivars used in this study. To develop improved methods, the effects on regeneration of an alternative cytokinin (TDZ) were investigated.…”

Section: Introductionmentioning

confidence: 99%

High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron

Gyves¹,

Sparks

Fieldsend

et al. 2001

Annals of Applied Biology

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Evening primrose (Oenothera spp.) is grown commercially for its seed oil that contains gamma linolenic acid (GLA), a valuable food supplement and pharmaceutical. There is considerable interest in the potential of genetic engineering to improve yields of GLA in evening primrose, and attention has focused on the current state of tissue-culture knowledge in this species which is a prerequisite for genetic transformation. Published protocols for the regeneration of plants from leaf or cotyledon material of Oenothera spp. are available, but these prove unsatisfactory when applied to commercial cultivars used in this study. An efficient method for regenerating three commercial cultivars of evening primrose Rigel, Merlin and Vulcan was developed using thidiazuron (TDZ) as a growth regulator. Explants from one month old seedlings were cultivated in vitro; a large number of buds were induced directly from strips of leaves, cotyledons and stems when cultured on Murashige and Skoog (MS) basal medium containing TDZ and indole-butyric acid (IBA). Shoots that were excised and placed onto MS basal medium, supplemented with IBA, rooted with 85-90% efficiency. Plantlets were transferred to soil after 6-8 wk. TDZ stimulated the regeneration process, and its effects were enhanced when combined with IBA or indole-3-acetic acid (IAA). The methods developed may be a useful advance toward improvement of this oil seed crop through genetic modification.Peer reviewe

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Tissue culture of wild-type, interspecific genome/plastome hybrids and plastome mutants of evening primrose ( Oenothera) : controlled morphogenesis and transformation

Cited by 8 publications

References 15 publications

Complete nucleotide sequence of the Oenothera elata plastid chromosome, representing plastome I of the five distinguishable Euoenothera plastomes

Complete nucleotide sequence of the Oenothera elata plastid chromosome, representing plastome I of the five distinguishable Euoenothera plastomes

Efficient in vitro regeneration of fireweed, a medicinal plant

High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron

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