Tissue culture of mammalian enteric neurons

Jessen, Kristján R.; McConnell, Janet D.; Purves, Robert D.; Burnstock, Geoffrey; Chamley-Campbell, J.

doi:10.1016/0006-8993(78)91112-5

Cited by 56 publications

(9 citation statements)

References 17 publications

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“…Techniques to isolate and culture the myenteric (Jessen et al, 1978, 1983; Korman et al, 1988; Nishi and Willard, 1985) or submucosal plexus (Surprenant, 1984) were developed in the late 1970s, and were even used for transplantation into the CNS (Tew et al, 1994). Unfortunately, most techniques isolated many non-ENS cells along with the ENS and the stem cells were only a small subset of the ENS cells isolated.…”

Section: Identifying Selecting Harvesting and Optimizing Isolatimentioning

confidence: 99%

White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies

Burns

Goldstein

Newgreen

et al. 2016

Developmental Biology

120

128

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Over the last 20 years, there has been increasing focus on the development of novel stem cell based therapies for the treatment of disorders and diseases affecting the enteric nervous system (ENS) of the gastrointestinal tract (so-called enteric neuropathies). Here, the idea is that ENS progenitor/stem cells could be transplanted into the gut wall to replace the damaged or absent neurons and glia of the ENS. This White Paper sets out experts’ views on the commonly used methods and approaches to identify, isolate, purify, expand and optimize ENS stem cells, transplant them into the bowel, and assess transplant success, including restoration of gut function. We also highlight obstacles that must be overcome in order to progress from successful preclinical studies in animal models to ENS stem cell therapies in the clinic.

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Section: Identifying Selecting Harvesting and Optimizing Isolatimentioning

confidence: 99%

White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies

Burns

Goldstein

Newgreen

et al. 2016

Developmental Biology

120

128

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“…Our own approach has been to develop tissue culture preparations of the myenteric plexus for combined structural and functional studies (Jessen et al 1978). These cultures have several advantages : they provide favourable monolayer preparations for immunohistochemical ( Fig.…”

Section: Gutmentioning

confidence: 99%

The changing face of autonomic neurotransmission*

Burnstock

1986

Acta Physiologica Scandinavica

262

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“…60 In addition, similar histological organization has been demonstrated for both brain and myenteric serotonergic systems. 61 The finding that stimulatory agents increase distal colon motility in low intestinal tone patients (Group II = hypomanic = catecholaminergic) correlates well with the headache attacks always evoked in them when these drugs were administered during the motility study. On the contrary, sedative (anti-CA) agents, which raise 5HT brain levels and decrease central and peripheral catecholaminergic activities, induced headache attacks when these drugs were administered to Group I subjects during the motility study.…”

Section: Discussionmentioning

confidence: 83%

Physiological, Clinical and Therapeutical Basis of a New Hypothesis for Headache

Lechín¹,

Dijs²

1980

Headache

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SYNOPSIS In the light of therapeutic, biochemical, physiological, pharmacological and clinical evidences, we postulate that the headache syndrome is due to an autonomic nervous system (ANS) imbalance, triggered by many aetiological factors. Consequently, this syndrome is most often observed in those subjects whose ANS balance is precarious, i.e. neurotics and psychotics. Two main types of headache patients could be differentiated: (1) those showing predominance of the serotonergic (5HT) over the catecholaminergic (CA) system (depressive patients) and (2) those showing predominance of CA over 5HT system (manic or hypomanic patients).

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Tissue culture of mammalian enteric neurons

Cited by 56 publications

References 17 publications

White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies

White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies

The changing face of autonomic neurotransmission*

Physiological, Clinical and Therapeutical Basis of a New Hypothesis for Headache

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