Tissue Culture of Desert Trees: I. Clonal Multiplication of Prosopis cineraria by Bud Culture

High frequency plantlets regeneration from seedling explants of Prosopis tamarugo

“…The percentage of such cultures was quite low (2-5%). In earlier attempts to regenerate and propagate P. cineraria, from mature and juvenile explants, a low number of axillary shoots per nodal explant were produced (Goyal & Arya 1981, 1984Yi et al 1989). Formation of a single embryo like structure on callus grown on B5 medium supplemented with 2, 4-D has been reported in Prosopis species (Jordan 1988) and Acacia koa (Skolmen & Mapes 1976).…”

“…The plant has been introduced to India with a view to reclaim saline desert soils (NBPGR Station,CAZR1,Jodhpur,Personal Communication). Reports are available on the establishment of callus and cell suspension cultures from a few species of Prosopis including P. tamarugo (Goyal & Arya 1981, 1984Jordan 1988). However, the technology is not available for the rapid and large scale multiplication of these species.…”

In Vitro Culture of Prosopis Species

“…Organogenesis and growth in vitro of P. cineraria have been reported earlier (11,12). Our work with P. tamarugo, P. chilensis and P. alba deals primarily with the induction of rhizogenesis to obtain plantlets from single shoot-node sections and shoot-tips in culture (17,18,19).…”

In vitro propagation of Prosopis species (P. chilensis, P. cineraria and P. juliflora)

“…). The use of tissue culture techniques to regenerate plants from nodal explants has also been reported for P. cineraria (Goyal and Arya, 1984), P. tamarugo and P. chilensis (Jordan and Balbao, 1985), and P, alba (Tabone et al, 1986) …”